Posted by admin on Oct 31, 2018 in |
Wounds may harbour diverse microorganisms, especially bacteria that are resistant to many conventional antibiotics. The aim of this study is therefore to evaluate the antibiotic activities of Aloe vera gel and honey against bacteria isolates from wounds. A cross sectional study of wound aspirates from a health care center was carried out to evaluate the antibacterial activities of Aloe vera and honey against bacteria isolates from wounds following standard microbiological procedures. The antimicrobial susceptibility of the isolates to standard antibiotic discs, Aloe vera and honey were done using the agar-diffusion method. The minimum inhibitory concentration of the Aloe vera gel and honey (alone and in combination) were also evaluated using the agar-diffusion method. The bacterial isolated were: Escherichia coli, Staphylococcus aureus, Proteus vulgaris, Staphylococcus epidermidis, and Streptococcus pyogenes. The study showed that honey had a higher antibacterial activity than Aloe vera gel with an inhibition zone diameter (IZD) measuring mm. It also showed that Staphylococcus aureus was the most predominant pathogen found in wounds while Proteus vulgaris and Staphylococcus...
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Posted by admin on Oct 31, 2018 in |
This study planned to screen preliminary phytoconstituents, total phenolic- flavonoids content and in-vitro, in-vivo antioxidant activities of aqueous crude extract of Camellia sinensis leaves. We assessed phytochemical constituents in extract using previously reported methods for tannins, phenols, flavonoids, glycosides, alkaloids and saponins. Quantitative estimation of flavonoids and phenolic compounds was carried out by aluminium chloride and Folin Ciocalteu method. ABTS, DPPH and FRAP methods are used for in-vitro assessment of antioxidant potential; meanwhile extract was tested for antioxidant capacity in Guinea pig plasma using ORAC, MDA and GSH assay in dose dependent manner. Total phenolic and flavonoid content of the extract was found to be 251.77 ± 6.59 µg/mg and 246.56 ± 2.58 µg/mg respectively. Extract shown 93.76 ± 12% antioxidant activity with ABTS and 95.13 ± 17.89% when tested with DPPH hydrogen reacting radicals. While, it was calculated 92.82 ± 8.72% for FRAP assay. The extract shows dose dependent decrease in MDA level (P<0.05, ANOVA) in plasma. Although, the extract produced dose depended increase in GSH value 20.97...
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Posted by admin on Oct 31, 2018 in |
A new reversed phase simple, economic and specific validated high performance liquid chromatography method for the estimation of empagliflozin in human plasma is presented. A chromatographic separation was carried out isocratically by mobile phase comprising of methanol and acetonitrile, 50:50% v/v at a flow rate of 1.0 ml/min on agilent long C18 column (250 mm × 4.6 mm, 5 µm) with photodiode arrays (PDA) detection at 270 nm. The bioanalytical procedure involves deproteination of plasma with 30% ethyl acetate and liquid-liquid extraction process. Chromatogram showed a peak of empagliflozin at retention time of 8.898 min. The correlation coefficient (r2) was found to be 0.999 in the concentration range of 50-150 μg/ml. No interference peak was observed in blank plasma samples at the retention time of empagliflozin. The percentage of relative recovery and coefficient of variation (CV %) values of precision and accuracy were within the acceptable limits. The method proved simple, cost effective and sensitive for estimation of empagliflozin in human plasma....
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Posted by admin on Oct 31, 2018 in |
The ancient medicine acupuncture was used to treat the diabetes and its complications. Our study was aimed to find out the hypoglycemic effect of Acu-TENS electrical stimulation at bilateral Zusanli (ST36) acupoint in streptozotocin (45 mg/kg/body weight/IP) induced diabetic rats. Diabetes was induced in male albino wistar rats with the administration of single dose of streptozotocin(45mg/kg/body weight/IP). The fasting blood glucose level, body weight, food and water intake were assessed periodically in the experimental animals and its effect was compared with conventional anti-diabetic drug (pioglitazone7.5 mg/kg/body weight). Zusanli acupoints was stimulated with 15HZ of 15MA high intensity and 5 sec pulse width for 5 minutes daily without anaesthesia for 60 days. Acupoint ST36 shows significant reduction (p≤0.01) in blood glucose level and also restored the body weight, food and water intake in treated rats and compared with anti-diabetic drug. The hypoglycemic effect of ST36 acupoint may be due stimulation of energy flow Qi to balance Yin and Yang and utilization of energy due to mild muscle twitching during stimulation....
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Posted by admin on Oct 31, 2018 in |
Over the counter (OTC) drugs are drugs easily available without the prescription of a registered medical practitioner (RMP) and self-mediation practices of these drugs are very common in medical students especially in diarrhea which mostly requires dehydration therapy only. But very few reports are available regarding the use of OTC antidiarrhoeal drugs. The aim of the study was designed to assess the practice of self-medication for diarrhea among second year medical students in RIMS, Ranchi, Jharkhand. This was a cross sectional study conducted among medical students in March-April 2017. Data was collected through self-administered questionnaire and result was expressed as percentage frequency. Questionnaires were filled completely by 224 students out of 269. 81.48% males and 85.07% females were practicing self-medication. Books were the major source of information about drugs and mild nature of illness was the main cause of self-medication practice. The most common drug group used for diarrhea was oral rehydration salt (ORS) and about 41.07% have used self-medication once in past 6 months. In 75% cases diarrhea...
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