Posted by admin on Aug 31, 2018 in |
Objective: Simple, rapid, sensitive, precise and reproducible specific UV spectrophotometric method for the determination of Aceclofenac (ACE) and Tramadol (TRM) in bulk drug and pharmaceutical dosage form were developed and validated. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. Results: UV-visible spectrophotometric method, measurement of absorption at maximum wavelength in 10 ml methanol and volume make with water solvent system as reference ACE and TRM were found to be at 203 nm and 241 nm respectively. The drug obeyed the Beer’s law and showed good correlation. Beer’s law was obeyed in concentration range 5 – 25 µg/ml for ACE and 2 – 10 µg/ml for TRM respectively with correlation coefficient was 0.999. The LOD and LOQ of ACE were found to be 4.7862 μg/ml and 14.50 μg/ml, TRM were found to be 2.0518 μg/ml and 6.2176 μg/ml, respectively. Percentage assay of ACE and...
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Posted by admin on Aug 31, 2018 in |
Green synthesis of nanoparticle using plant extracts is an efficient alternative for the conventional process of preparation of nanoparticles by chemical and physical methods which leads to non-eco-friendly by-products.The main aim of the project was to produce silver nanoparticles (AgNPs) using leaf extracts of Ayapana triplinervis (Eupatorium triplinerve) a medicinally important herb and to ascertain its antibacterial properties. AgNPs were synthesized by adding 1 ml leaf extract of A. triplinervis to 10ml of 3mM silver nitrate followed by incubation at 60οC. The AgNPs produced were further characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) analysis. UV-Vis spectroscopy of prepared silver colloidal solution showed absorption maximum between 460 – 490nm due to the excitation of surface plasmon resonance (SPR) of the AgNPs. FTIR spectra absorption peaks at 3431, 2065, 1635 and 530 cm−1 revealed the availability of functional groups which may be involved in the AgNPs synthesis. The SEM analysis showed that AgNPs were spherical or ellipsoidal in shape.The antibacterial activity against common...
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Posted by admin on Aug 31, 2018 in |
Ipomoea sepiaria Roxb., also known as Purple heart glory is a slender vine belonging to family Convolvulaceae. It is one among the ten sacred plants known collectively as “Dasapushpam” in Kerala. These plants are used for rejuvenating the body in the form of ‘karkidakakanji’ in the monsoon season in Kerala. Juice of the plant is used as deobstruent, diuretic, hypotensive, uterine tonic and antidote to arsenic poisoning. The present study aims at providing a detailed information of microscopic features of the leaf and stem. In cross sectional view the leaf exhibits short adaxial core, semicircular abaxial midrib and smooth lamina. The vascular bundle of the midrib is single, planoconvex and collateral. The vascular bundle includes wide, angular compact metaxylem elements and narrow circular protoxylem elements. Prominent glandular trichomes occur frequently on adaxial and abaxial epidermal layers. The glands of these trichomes are capitate type. The stem is circular, with short conical spiny ridges at certain places. It consists of intact epidermis, narrow cortex, elliptical hollow cylinder vascular tissues and...
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Posted by admin on Aug 31, 2018 in |
Absence of pathogenic bacteria is a must for passing the microbiological quality control inspection of non-sterile pharmaceutical products. The identification of microbial contaminants by conventional methods is time-consuming and labor-intensive. The overall aim of the study was to test non-conventional methods as polymerase chain reaction (PCR) and Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectroscopy Biotyper (MALDI-TOF) for rapid identification of Pseudomonas aeruginosa and Staphylococcus aureus in topical pharmaceutical preparations. Four different topical preparations and three raw materials were artificially inoculated with tested microorganisms, at an initial cell count of 10º CFU/ml, and were overnight pre-enriched in Tryptic soy broth (TSB). MALDI-TOF was tested for identification of target microorganisms using different sample preparation methods. Duplex PCR targeting oprL (Pseudomonas aeruginosa) and mRNA nuclease gene (Staphylococcus aureus) was also tested. Pseudomonas aeruginosa and Staphylococcus aureus were identified within 18 – 37 h using MALDI-TOF, according to the sample preparation method, and 27 h using duplex PCR; compared to 48 – 72 h for the identification using conventional microbiological...
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Posted by admin on Aug 31, 2018 in |
Present study is reporting the result of the synthesis and evaluation of antihypertensive activity for N-substituted pthalazine 1,4-dione derivatives. N-substituted pthalazine 1,4-dione derivatives were synthesized by taking the Heterocyclic system, ethylglycinate, hydrazine hydrate and pthalic anhydride and were subjected to preliminary screening for antihypertensive activity in albino rats. The synthesized compounds were characterized on the basis of their spectral (1H NMR, 13C NMR, Ms, IR) analysis. Antihypertensive activity of all the compounds was tested by non-invasive method using Tail Cuff method and all the compounds were found to be active. The significance of the work is to treat cardiovascular disorder implicated by Steroids and...
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