Posted by admin on Oct 31, 2018 in |
Chromatographic method was developed according to Quality by Design (QbD) approach as per ICH Q8 (R2) guidelines for estimation of pitavastatin in pharmaceutical dosage form. By considering the current regulatory requirement for an analytical method development, a reversed phase high performance liquid chromatographic method for routine analysis of Pitavastatin has been optimized using analytical quality by design approach. Unlike routine approach, the present study was initiated with understanding of quality target product profile, analytical target profile and risk assessment for method variables that affect the method response. A liquid chromatography system equipped with a C18 column (250 × 4.6 mm, 5 µ), a binary pump and photodiode array detector were used in this work. The experiments were conducted based on plan by central composite design, which could save time, reagents and other resources. Sigma Tech software was used to plan and analyses the experimental observations and obtain quadratic process model. The process model was used for predictive solution for retention time. The predicted data from contour diagram for retention...
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Posted by admin on Oct 31, 2018 in |
Fruits play an important role in human life because it provides valuable nutrients such as carbohydrates protein and fibres. Fruits are commercially processed to produce fruit juices, jams, jellies etc. Fruit juices sometime naturally contaminates with certain substances like carbohydrates, heavy metals etc. However, sometime manufacturer intentionally adds some substance like carbohydrates and acids to increase the life span of the products and vitamin C to increase product sale. Presence of carbohydrates and acid helps in the quality assessment of the fruit juices. Color tests mainly used for the identification of carbohydrates such as sugar, reducing sugar and invert sugar etc. Titration is a common laboratory method of quantitative/chemical analysis that can be used to determine the unknown concentration of a known reactant. In the present study, total six branded mango juice samples were collected from local market of Gandhinagar. Acid content, fruit content, reducing sugar, total reducing sugar and glucose: fructose ratio and vitamin C were analysed with titrimetric method and this were found in the range of...
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Posted by admin on Oct 31, 2018 in |
Like most other plants Citrus paradisi contain various secondary metabolites with great potentials. The aim of this paper is to evaluate the phytochemicals by using quantitative and qualitative analysis of ethyl acetate, ethanol, n-hexane and aqueous extracts with the help of standard techniques. The findings from quantification and phytochemical screening showed the presence of alkaloids, flavonoids, reducing sugars, Phenols, proteins, amino acids, saponins, tannins, terpenoids, and glycosides. Further, the study findings revealed that ethanolic extract of fruit extract was found to have more constituents when compared with other extracts by quantitative method. Elemental analysis showed the presence of selenium (1.56 mg); manganese (4.95 mg); iron (5.12 mg); magnesium (34.5 mg) and zinc (9.85 mg). Chromatogram of flavonoid standards such as rutin, quercetin, gallic acid, hesperidin and ethanolic extract of Citrus paradisi showed the high amount of naringin. Hence the ethanolic extract of Citrus paradisi shows many compounds and may have been used in traditional medicine for prevention of several...
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Posted by admin on Oct 31, 2018 in |
Berberine hydrochloride is an isoquinoline alkaloid isolated in the form of Berberine hydrochloride from the roots and stems of Berberis vulgaris, B. aristata, Coptidis rhizoma and other plant species. Berberine is well known for its antiproliferative and anti inflammatory activity since long time. The present study is carried out to study mechanism of inducing apoptosis by berberine hydrochloride on HEPG2 liver cancer cell line. MTT assay used to study cytotoxicity against HEPG2 cells and COX2, PGE2 and western blot were used to study its apoptotic pathway. PGE2 has been implicated in COX-2-mediated effects; we determined the levels of PGE2 in the berberine-treated cells. Our results revealed that treatment with berberine hydrochloride for 24 h resulted in significant inhibition of PGE2 production in a dose-dependent manner 21-75%, P, 0.01-0.001, suggesting that berberine induced reduction in PGE2 production is associated with an inhibitory effect of the berberine on COX-2 in these cells. Berberine has shown PPAR Gamma activation and COX2 inhibition via PGE2 regulation and caused apoptosis by down regulating anti-apoptotic...
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Posted by admin on Oct 31, 2018 in |
Aim of the Study: The aim of this study was to investigate the antilithiatic activity of ethanolic leaves extract of Dalbergia sissoo Linn. ethylene glycol induced in rats. Materials and Methods: All the chemicals were analytical grade. Cystone was obtained from from Himalaya Health Care Ltd. The ethanolic extract of plant Dalbergia sissoo Linn. leaves were given with tween 80 as vehicle administered orally. All chemicals were used of analytical grade available; ethylene glycol was obtained from Merck Ltd., Mumbai, India. Male Albino rats of wistar strain weighing between 150-200 g were selected for the antiurolithiatic activity. Animals were housed in polypropylene cages with the filter tops and maintained at 25 ± 2 ºC, relative humidity 55 ± 10% under controlled conditions of 12-h light: 12-h dark cycle. Results: In the present in-vivo study DSEE (at 100 and 200 mg/kg) exhibited a dose dependent significant anti-lithiatic activity on treatment. The extract dose of 100 mg/kg also caused reduction of calcium, oxalates, phosphorus and creatinine in blood serum level the...
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