Posted by admin on Oct 31, 2018 in |
The approach of site specific topical delivery of Rizatriptan Benzoate (RBZ), used for migraine using the elastic carrier at posterior cervical region for sustained action was attempted in the present study. RBZ-entrapped elastic niosomes were prepared using the modified mechanical stirring method. The formulation was optimized using 23 full factorial designs. The type of edge activator (EA), Tween: cholesterol: edge activator molar ratio (T:C:EA) and Total surfactant concentration (S) as independent and the percent entrapment efficiency (%EE) as dependent variables with visual stability of dispersion for two week as constrain factor for selection. The study revealed that interaction between EA and S was influencing factor for %EE. The optimized formulation EN3 having edge activator type, total surfactant concentration and molar ratio Tween 20: cholesterol: EA as Sodium cholate, 2% and 2:1:2 respectively, having high percent entrapment efficiency (92.22%), showed unilamellar smooth surface in SEM study. Zeta potential was found to be – 17.2 mV. In-vitro permeation from the elastic niosomal gel followed zero order model over a period of...
Read More
Posted by admin on Oct 31, 2018 in |
In the current study, synthesis of silver nanoparticles (AgNPs) using swertipunicoside was tested against human breast cancer cell line (MCF-7). The swertipunicoside was tested against the normal cell line such as vero cells to find out the toxicity of the compound. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), fourier-transform infrared spectroscopy (FTIR) and field-emission Scanning Electron Microscope (FESESM) with energy dispersive spectroscopy (EDAX). The synthesized silver nanoparticles were checked with the colour variation and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles were analysed using FESEM along with EDAX. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using swertipunicoside. The synthesized AgNPs and swertipunicoside was tested against the MCF-7 cell line to find out the cell viability. Our results showed that the compound and synthesized silver nanoparticles inhibited the proliferation of human breast...
Read More
Posted by admin on Oct 31, 2018 in |
Cajanus cajan L. (Pigeonpea) is an important pulse crop of India. The aim of this study was to determine and compare the antioxidant activity and bioactive compounds of seed coat and cotyledon of C. cajan. The antioxidant properties of pet ether, chloroform, ethanol and aqueous extracts of the seed coat and cotyledon of C. cajan were examined by a ferric thiocynate (FTC) assay, DPPH radical scavenging assay, hydroxyl radical scavenging assay and ferric reducing power were analyzed separately for its inhibition percentage at different concentration (20 µg, 40 µg, 60 µg, 80 µg and 100 µg/ml). Both the samples possessed antioxidant activity however the seed coat ethanolic extract possessed prominent activity by FTC (68.2 ± 0.07%), DPPH (90.1 ± 0.17%) and hydroxyl radical scavenging assay (75.2 ± 0.05%) at 100 µg/ml compared to other extracts. It was interesting to note that ferric reducing power of ethanolic extract of seed coat (1.590 ± 0.002%) was significantly (P<0.5) higher than that of standard BHA (1.575 ± 0.015%) at 100 µg/ml. The...
Read More
Posted by admin on Oct 31, 2018 in |
Investigation of antioxidant, anti-inflammatory and antimicrobial activity of a dental film, which easily reabsorbs with applied phytocomplex of Geranium palustre, has been performed to use it for the treatment of oral mucosa diseases. To get the extract, the dried material was grounded, extracted and lyophilized. Quantitative determination of biologically active substances in lyophilized phytocomplex was performed on spectrophotometer and high performance liquid chromatograph. For investigation of antioxidant properties of the extract, the method, based on the interaction with stable chromogen-radical DPPH, was used. Investigation of anti-inflammatory activity of a dental film with extract of Geranium palustre herb was carried out based on carrageenin model of inflammatory. The study of antimicrobial properties of extract was performed using laboratory strains of microorganisms as a test-culture. The obtained qualitative indices of biologically active substances of the investigated phytocomplex showed high content of tannins, polyphenol compounds, polysaccharides, phenolcarboxylic acids, organic acids, ascorbic acid, free amino acids and other compounds, due to which lyophilized extract possesses a wide range of properties, important for treatment:...
Read More
Posted by admin on Oct 31, 2018 in |
Organophosphate pesticides are associated with neurotoxicity. Organophosphates are cholinesterase-inhibiting chemicals used predominately as pesticides. These have played a very important role in sustainable production of food, animal feed and as protection against disease vectors. They are also used as chemical warfare agents (nerve agents). The effects of dichlorvos and monocrotophos on brain neurotransmitters are well established. The combined effect of organophosphates (dichlorvos and monocrotophos) was supposed to have synergistic or additive effect on brain neurotransmitters. Combined exposure of dichlorvos and monocrotophos has significant inhibition in acetylcholine esterase activity during both studies of 15 days and 30 days. Levels of nor-epinephrine, dopamine and serotonin (5-hydroxytryptamine) increased significantly in dichlorvos and monocrotophos exposed animals. However, the increase was more pronounced in monocrotophos alone exposed animals. Thus, contrary to hypothesis, the combined exposure to these toxicants did not produce more pronounced toxicity as compared to their individual exposure except in case of...
Read More
