Posted by admin on Sep 30, 2018 in |
The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation by pre derivatisation technique using reverse phase C18 column in HPLC. In this RP-HPLC method Shimadzu LC 20 AD of PDA detector was used with LC solutions software. In this method gradient elution with a mobile phase of acetonitrile and acetate buffer in the ratio of 25:75 v/v at the flow rate of 2 mL/min and run time of 10 min were used. Validation parameters of HPLC were found at detection wavelength of 272 nm. Linearity was observed with a concentration range of 10 – 50 µg/mL with R2 = 0.99. The method was robust with wavelength 271 and 273 nm with a flow rate of 1.8 and 2.2 mL/min showed good results. The retention time of drug was found at 8.9 min and assay showed 98.1%. RP-HPLC method was validated as per ICH guidelines and can be used for routine quantitative analysis of...
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Posted by admin on Sep 30, 2018 in |
A simple and rapid stability indicating HPLC method for acotiamide hydrochloride was developed and validated. The stationary phase used was thermo gold cyno (250 × 4.6 mm) 5 μ with a mobile phase consisting of mixture of acetonitrile and 10 mM KH2PO4 in water in the ratio of 80:20 v/v at a flow rate of 0.8ml/min. Detection was carried at 284 nm. The retention time observed was 8.1 ± 0.2 min. The column was maintained at ambient temperature and 20 μl of solutions were injected. The eluted compound was detected by using PDA detector. The linear regression analysis data for calibration plot show good relationship with coefficient of regression value, r2=0.99 in the concentration range 10-50 μg/ml. The stress degradation studies were performed as per ICH guidelines. Acotiamide hydrochloride degraded with acid/base hydrolysis, thermal, oxidation and photolytic stress. The method was validated as per ICH Q2A (R1) guideline with respect to linearity, accuracy, precision, specificity and robustness. This method can be used for monitoring the stability of acotiamide...
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Posted by admin on Sep 30, 2018 in |
The objective of the study was to develop mesoporous silica nanoparticles for the poorly water soluble drug ritonavir (RTV) for enhancement of in-vitro dissolution and corresponding in-vivo bioavailability. A comparative assessment between 2D-hexagonal silica nano-structured MCM -41NPs and 3D cubic pore system MCM -48NPs on drug release rate was also investigated. RTV (BCS class II drug), was loaded in the synthesized MCM-41NPs and MCM-48NPs by the solvent evaporation technique. The obtained MCM-41NPs, MCM-48NPs and RTV loaded mesoporous nanoparticles were characterized by different analytical techniques like UV spectrophotometry, differential scanning calorimetry, thermogravimetric analysis, FTIR, N2 adsorption-desorption technique, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and powder-XRD. The in-vitro release profile of RTV was studied in 900 mL 0.1N hydrochloric acid (HCl) medium using USP apparatus-II at 50 rpm. Further; In-vivo studies were performed in Wistar rats where drug loaded mesoporous nanoparticles were compared with pure RTV. In dissolution study MCM-48NPs showed better and fast release of RTV than the MCM-41NPs. In pharmacokinetics study, the maximum peak plasma concentrations of...
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Posted by admin on Sep 30, 2018 in |
Benzosuberones and phenylpiperazinyl moieties are important heterocyclic compounds which constitute many biological activities which include anti bacterial, anti fungal, anti inflammatory, antioxidant, antitubercular and anti cancer activities. A novel series of 2,3-dimethyl-N-(3-(4-phenyl piperazin-1-yl)propyl)-6,7-dihydro-5H-benzo[7] annulene-8-carboxylic acids (5a-e) have been synthesized in good to excellent yields by experimental simplicity and milder reaction conditions and evaluated for their anti-proliferative activity. The titled compounds were identified and characterized by IR, 1H NMR, 13C NMR and mass spectral analysis. The novel derivatives synthesized were evaluated for in-vitro anti-proliferative activities on four different human cancer cell lines He La (cervical), MIAPACA (pancreatic), MDA-MB-231 (breast) and IMR32 (neuroblastoma) by sulphorhodamine B assay method. These synthesized novel derivatives which showed excellent anti-proliferative activity. The obtained results suggest that these classes of compounds can be considered as new hits for further structural optimization to obtain better anti-proliferative drug development...
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Posted by admin on Sep 30, 2018 in |
Studies to exploit wild mushrooms as a source of biologically active compounds are gaining importance in the recent years. In that view, the present study was carried out to evaluate the bioactives of wild edible macrofungi, Lentinus squarrosulus Mont. The specimen was subjected to various biochemical tests with the view to establish standards for their identity and chemical composition. To identify the chemical constituents the dried sporocarp was subjected to successive solvent extraction like petroleum ether, chloroform and ethanol using a Soxhlet extractor. The qualitative mycochemical analysis have shown the presence of some important chemical constituents viz., alkaloids, terpenoids, flavonoids, cardiac glycosides, steroids, phenols, fats and oils in all the extractives. Further, GC-MS analysis of ethanolic extracts was carried to identify the important volatile constituents. This data may provide baseline information about the bioactive constituents of these species in providing valuable compounds of substantial medicinal importance. The study also envisaged the proper identity of the target specimen by molecular identification. The present study illustrates the value of molecular tools,...
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