Posted by admin on Jul 31, 2018 in |
The pretentious extract of Pseudomonas aeruginosa has shown promising anticancer activity. It contains a protein called Azurin. It has a molecular weight of 14 kDa. It is soluble in water. It is a type I copper-containing protein of cupredoxin family. In this work, Pseudomonas aeruginosa MTCC strain 647 was cultivated in modified Asparagine -proline broth. An attempt was done to isolate and purify azurin from Pseudomonas aeruginosa (MTCC 647) by using chromatography on sephadex G, CM cellulose and SDS PAGE electrophoresis. Recent studies have found that Pseudomonas aeruginosa can synthesize nano particles through either intracellular or extracellular mechanisms by bio synthetic means. Hence, the composite nano particles (Azurin-Ag NP) were synthesized by biosynthetic methods. P. aeruginosa MTCC strains 647 was cultivated in modified Asparagine -proline broth initially to produce shake flask cultures and then fed batch cultivation. From the bacterial extracted Azurin is isolated and purified to get a final concentration of 4.6 mg/g dry bacteria. As the nano particle mediated drug delivery effectively deliver the drug to the...
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Posted by admin on Jul 31, 2018 in |
A series of tetrazol derivatives 1(a-c) and 2(a-c) were synthesized by Mannich base reaction. Synthesized compounds 1(a-c) and 2(a-c) were confirmed by IR, ¹H NMR, ¹³C NMR, mass spectral, and elemental analysis. Synthesized compounds 1(a-c) and 2(a-c) were screened for dental plaque bacteria and cytotoxicity activity. The compound 1b was highly active against Enterococcus feacalis in antibacterial screening. The synthesized compounds have been screened for preliminary cytotoxicity against HepG2 (Liver), Hela (Cervical) and MCF-7 (Breast) cancer cells. The compound (1c) is highly active against MCF-7(Breast), and compound (2b) is highly active against HepG2 (Liver). Therefore, current study demonstrates the antibacterial and cytotoxicity activity potential of new tetrazole derivatives and provides future insights for developing dental plaque antibacterial...
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Posted by admin on Jul 31, 2018 in |
The objective of the present study was to evaluate the effect of microwave sintering condition on matrix formation and subsequent drug release from polymer matrix tablet for controlled release. The Atenolol floating SR matrix tablets were prepared by using tragacanth, sodium bicarbonate, Eudragit S-100 and citric acid was incorporated as a gas generating agents. Atenolol controlled release tablets were estimated in terms of their physico-chemical parameter like weight variation, friability, hardness, drug content, contact angle, floating lag time in-vitro drug release, specific gravity and stability study as per ICH guidelines. The results of in-vitro drug release studies showed that the optimized formulation (F6, L2, I2, H4) could extend drug release (99.5%) for 24 hours. The release pattern of Atenolol was fitted to different models based on coefficient of correlation (r). The present work also deals with the application of sintering technique to matrix tablets of Atenolol to achieve sustained release. The results of the present work stated that sintering caused decrease in drug release as compared to unsintered tablets....
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Posted by admin on Jul 31, 2018 in |
The study was conducted to evaluate the synergistic effect of EDTA with antibacterial (Ceftriaxone + Sulbactum) as a combinational therapy against antioxidant enzyme activity, lipid peroxidation level, some hematological and biochemical parameters in Klebsiella pneumoniae induced nosocomial pneumonia followed by histopathology of lungs tissue in experimental exposed Sprague Dayle (S.D) rats. Total 24 rats were selected and divided into 4 groups of 06 rats each for in-vivo and in-vitro EDTA / antibacterial therapy. Group I Control normal saline treated group; group II was infected via K. pneumoniae bacterial strain. Group III and IV were infected plus treated group with antibacterial and EDTA / Antibacterial drugs. The results indicated that a significant (p<0.005) decrease in enzymes activities (Superoxide dismutase, catalase and ascorbic acid) along with increased lipid peroxidation level as well as cytokine parameters (tumour necrosis factor-α, interlukin-6) and biochemical parameters in plasma of infected group as compared to control group. These activities were increased along with decreased in lipid peroxidation level and biochemical parameters in both treated group as...
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Posted by admin on Jul 31, 2018 in |
The adverse effects of oxidative stress have been discussed widely in the present day. It is the main cause of protein denaturation, cardiovascular diseases, ageing, etc. Thus, the daily requirement of anti-oxidants has to be fulfilled through dietary sources. Inflammation is yet again another form of stress on the body. It is the major cause of multiple chronic disorders. It can lead to the formation of many other disorders such as arthritis, asthma, macular degeneration, etc. Arthritis is one such inflammatory disorder which is associated with swelling of joints, joint pain, etc. This research was based on the anti-oxidant, anti-inflammatory and anti-arthritic properties of tea extracts such as Camellia sinensis, Hibiscus rosa sinensis, Rosa, Zingiber officinale, Matricaria chamomilla. Ex-vivo tests for evaluating antioxidant property of samples using liver tissue by LPO assay was performed. In addition, tests such as inhibition of protein denaturation and RBC membrane stabilization were performed to determine anti-inflammatory effect of the sample. Collagen denaturation assay using chicken collagen to check anti-arthritic properties of samples were...
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