Posted by admin on Aug 31, 2018 in |
Atherosclerosis is a condition where the arteries become narrowed and hardened due to an excessive build up of plaque around the artery wall. The disease disrupts the flow of blood around the body, posing serious cardiovascular complications. Liver X alpha receptor (LXRα) is highly expressed in liver, function as cholesterol sensors with important roles in regulating cholesterol homeostasis. The leaves of Corchorus trilocularis possess anti-atherosclerotic property along with its anti hyperglycemic activity. The Genetic Optimization for Ligand Docking (GOLD) software resulted in identifying the best compound that interacts with the receptor. The present study was carried out to evaluate the anti-atherosclerotic activity of the ethanolic leaf extract of Corchorus trilocularis against LXRα by...
Read More
Posted by admin on Aug 31, 2018 in |
A LC-MS method was developed and validated for determination of ergocalciferol in human plasma. n-hexane was used as extraction solvent while kinetex C18 50*2.1 mm, 1.7µm was used as analytical column for chromatographic separation. Mobile phase was acetonitrile: 0.1% formic acid in methanol (90:10). Turbo ion spray with positive polarity was used as an interface with API QTRAP 5500 LC/MS/MS system. The calibration range was validated for 2.03 ng/mL to 150.69 ng/mL. Limit of quantification was 2.03 ng/mL. The recovery of Analyte was 97.04% to 99.27%. Labeled erythromycin was used as an internal standard for this method. Inter-batch and intra-batch accuracy and precision were calculated to check method’s performance. Stability was established for all anticipated working conditions for analyte as well as internal standard. Apart from stability experiments, other experiments were also performed to simulate the conditions of subject sample analysis. This method was applied successfully on bioequivalence study to evaluate pharmacokinetic...
Read More
Posted by admin on Aug 31, 2018 in |
The present study was conducted to investigate protective effect of hydro-methanolic (2:3) extract of leaf of Abroma augusta in streptozotocin induced diabetic male albino rat. For this purpose animals were divided into three treatment groups (n = 6): control group, STZ induced diabetic group (STZ 4 mg / 0.1 ml citrate buffer/ 100 g body weight), hydro-methanolic extract of leaf of Abroma augusta treated group (20 mg / 0.5 ml distilled water/100 g body weight). Treatment was conducted for 28 days. Blood glucose, serum insulin, glycated haemoglobin levels along with activities of hepatic carbohydrate metabolic enzymes, hexokinase and glucose-6-phosphate dehydrogenase were assed in all experimental groups. Oxidative stress markers like catalase, peroxidase enzyme activities, conjugated diene and thiobarbituric acid reactive substance levels in liver were measured. All these parameters were significantly recovered towards the control level after the treatment of hydro-methanolic extract. Pancreatic islet diameter, count of islet and number of cells in islets also decreased in diabetic animals. Treatment of these animals with extract resulted significant recovery towards...
Read More
Posted by admin on Aug 31, 2018 in |
The whole plant of Cocculus pendulus (J.R. & G. Forst.) Diels is reported to have quality medicinal values in traditional system of medicines. The various physico-chemical parameters included determination of extractive value, loss on drying, ash values, fluorescence analysis, HPTLC analysis and preliminary phytochemical analysis were performed. The preliminary identification of Cocculus pendulus was observed by studying macroscopical features and the final authentication was confirmed by the botanist. Preliminary phytochemical analysis of whole plant of Cocculus pendulus revealed the presence of various phytoconstituents like alkaloids, carbohydrates, phytosterols, saponins, proteins and mucilage. HPTLC fingerprint analysis of ethanolic extract of plant Cocculus pendulus reveals presence of 13 peaks and 10 peaks in 256 nm and 366 nm respectively. The physico-chemical evaluation of Cocculus pendulus was a useful tool to identify quality, purity and authenticity of Cocculus...
Read More
Posted by admin on Aug 31, 2018 in |
The present work aims to design, synthesize some novel benzimidazole derivatives and to perform in-vitro evaluation of their antitubercular and antimicrobial activity. In-silico modelling of benzimidazole derivatives was carried out using various softwares such as, ACD Labs Chemsketch, Molinspiration, PASS (Prediction of activity spectra for substances) and Schrodinger Glide XP (Grid based ligand docking with energetics). Nine derivatives (BI 1a, 1b, 1c, BI 2a, 2b, 2c and BI 3a, 3b, 3c) were designed. The designed molecules with required physicochemical properties, drug likeness and obeying Lipinski’s rule of five were selected for the synthesis. The synthesized compounds were subjected to TLC, melting point determination, FTIR and mass spectral studies. All the synthesized compounds showed characteristic peak in FTIR and Mass spectroscopic studies. As per the PASS score and GLIDE score the compounds were selected for biological studies like antitubercular, antibacterial, and antifungal activity. These results are useful for further investigation in the future. All the selected derivatives showed better activity, when compared with standard...
Read More
