Posted by admin on Jul 31, 2018 in |
Proteomic study of Macrotyloma uniflorum is quite useful for exploration of its Calcium binding proteins (CBPs) and their antilithiasis character. CBPs play an important role in prevention of kidney stone formation. Considering this fact the study was design to identify CBPs in M. uniflorum. Total six samples were collected from different elevations of Uttarakhand. Total buffer soluble proteins of seeds were extracted according to the standard protocols. After quantification of protein with Bradford method 20 µg protein was loaded in 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel according to Laemmli discontinuous system. CBPs were identified with the help of stains-all gel staining methods. In the proteomic study a protein (~44KDa) showed its distinct appearance when screened, the seeds from high elevation to low elevation. Three blue bands (CBPs) appear in all samples after stains-all staining. One high molecular weight protein (~43KDa) and two low molecular weight proteins (~25.1 and ~17KDa) respectively were observed. SDS-PAGE technique of seed proteins can be economically used to broad genetic variation and...
Read More
Posted by admin on Jul 31, 2018 in |
Naturally occurring safe and broad spectrum UV blockers are required now- a- days to protect oneself from the onslaught of UV radiations that are known to cause many skin related ailments. The commercial inorganic sunblockers like Zinc oxide and titanium di oxide and organic sunblockers like Avobenzone and Benzophenone are known to be unstable and also their safety is doubted. Aim of the present communication was to study the secondary carotenoids like Zeaxanthin / Canthaxanthin / b– carotene extracted from thermotolerant genera Synechocystis pevalekii is screen for its role in skin protection against UV radiations. Solvents like Petroleum ether, Hexane, acetone, ethyl acetate, ethanol and methanol were used for extraction. Out of all these, freeze – thaw technique in 100% methanol proved to be the best solvent for extraction of carotenoids. The crude extract thus obtained was fractionated by silica gel open column chromatography and the resultant fractions were analyzed by HPLC. The HPLC analysis showed the presence of carotenoids like Zeaxanthin, Canthaxanthin and b- carotene. The next part...
Read More
Posted by admin on Jul 31, 2018 in |
A new, simple, and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative determination of Carbamazepine. Carbamazepine was chromatographed on silica gel 60 F254 TLC plate using Ethyl acetate: Methanol (9: 1 v/v) as mobile phase. Carbamazepine was quantified by densitometric analysis at 239 nm. The method was found to give compact spots for the drug. The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range 100 – 600 ng/spot. The method was validated for precision, recovery, accuracy, and robustness as per the International Conference on Harmonization guidelines. The minimum detectable amount was found to be 15.096 ng/spot, whereas the limit of quantitation was found to be 45.696 ng/spot. Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the analysis of Carbamazepine. The method was successfully employed for the estimation of equilibrium solubility, quantification of Carbamazepine as a bulk drug, in commercially available...
Read More
Posted by admin on Jul 31, 2018 in |
Excess fluoride causing fluorosis, can enter in to brain, disturb oxidative status and induce neural damage. Quercetin, antioxidant flavonoid stabilizes oxidative milieu of brain by quenching free radicals. The present study aimed to report ameliorative effect of quercetin on NaF induced oxidative stress, histological alterations in the brain and behavioural changes of rats. Rats were divided into four groups. The first group served as normal which received tap water. The second group was intoxicated with sodium fluoride (20 ppm/kg bw) through drinking water. The third group was given 20 ppm sodium fluoride in drinking water, along with treatment of quercetin (20 mg/kg bw) through oral gavage. The fourth group was treated with quercetin (20 mg/kg bw) through oral gavage. All animals were maintained for 60 days. Then, the antioxidant enzyme levels were measured, histopathological and behavioural studies were carried out. The results showed that the quercetin treatment significantly reversed the NaF induced increased lipid peroxidation (LPO) and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels in the...
Read More
Posted by admin on Jul 31, 2018 in |
The aim of study was to evaluate cardioprotective activity of fulvic acid in doxorubicin induced cardiac toxicity. In current investigation, female/male Wistar rats were divided into five groups, normal, control, fulvic acid 100 mg/kg, fulvic acid 200 mg/kg, and fulvic acid 300 mg/kg. Doxorubicin 15 mg/kg was administered in six divided doses to all except normal group for 2 weeks. Fulvic acid was administered to respective groups once daily for total 21 days, on the last day of study, the animals were anesthetized to record ECG and BP (by cannulating carotid artery), blood was collected from carotid artery and SGOT, LDH and CK-MB were estimated, animals were sacrificed to isolate heart and preparation of tissue homogenate. The antioxidant status is analyzed by measuring MDA content, SOD, CAT, GSH activity. The tissue sample is also preserved for histological studies. Doxorubicin cause cardiac damage which was manifested by alteration in serum cardiac markers, antioxidant markers, ECG and hemodynamic and histological changes. Which were assuaged due to treatment with fulvic acid 300...
Read More
