Posted by admin on Jun 30, 2018 in |
Polyherbal preparations (PHP) are known to express high effectiveness in a vast number of diseases. In view of this, present investigation was carried out to identify phytochemical constituents of PHP, prepared from the aqueous extract of test plant powders of Mimusops elengi Linn., Strobilanthes barbatus Nees, Indigofera zollingeriana Miquel and Dillenia indica Linn. Phytochemical screening of APHP revealed the presence of carbohydrates, Proteins and amino acids, alkaloids, glycosides, phenols, flavonoids, and fixed oils/fats. Acute toxicity studies were conducted on female wister rats. Study design is according to OECD Guidelines no. 423. APHP was administered at dose of 5 mg/kg, 50 mg/kg, 250 mg/kg, 500 mg/kg, 1000 mg/kg and 2000 mg/kg body weight as suspension (1% Na CMC) along with blank. No-Observed-Adverse-Effect- Level (NOAEL) of APHP was found to be 2000 mg/kg. In connection to that, sub chronic toxicity studies have been carried out to predict the dose to be selected for in-vitro and in-vivo experiments. APHP at the doses of 250 mg/kg, 500mg/kg and 1000mg/kg were administered and examined...
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Posted by admin on Jun 30, 2018 in |
Diethylstilbestrol (DES) is an endocrine disturbing compound it can mimic and perform like a natural hormone, which mimics estradiol by binding to the estrogen receptor. This compound mainly reproductive target, so in this study used to induce endometriosis. Endometriosis is an estrone dependent constant gynaecological disorder. Here it, intends to induce endometriosis and treated by the MMP inhibitor to check whether it could attain the implantation or not. Hence the present study induces endometriosis by three different concentrations such as 10, 15 and 20 µg/kg body weight for 20 days at alternatively while to check the estrous cycle, hormonal regulation at 7th, 15th and 20th day also to record the body weight and reproductive organ weight from initial to final day of DES induction. Endometrial lesion developments were confirmed by anatomical observation. The above result reveals 20 µg DES induced group leads to endometrial cancer, so other 10 & 15 µg DES induced group carry out to further analysis. The endometrial lesions were treated by 10 µM concentrations of...
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Posted by admin on Jun 30, 2018 in |
Cancer, the uncontrolled growth of cells, has been estimated as a major cause of death among human population. Although there are many potential drugs, a much effective bioactive drug with less side effects are still in its pioneer stage of research. The aim of the study was to isolate, purify and characterize the anticancer muscle protein from edible marine catfish, Tachysaurus dussumieri, so as to project it as an alternative medicine for cancer. The anticancer muscle protein was purified to its homogeneity from the edible marine catfish Tachysurus dussumieri through ammonium sulphate fractionation and ion exchange chromatography. Both partially purified crude fractions and isolated purified muscle protein were screened for its anticancer activity by MTT assay. The fraction III revealed prominent anti-cancer activity which was subjected to MALDI-TOF MS analysis for its molecular weight confirmation. Further, Peptide Mass Finger printing analysis (PMF) was executed to reveal its nature. To reconfirm its anticancer action, DAPI, PI staining and Comet assay were executed. The purified dimer having 13.285 kDa weight was...
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Posted by admin on Jun 30, 2018 in |
A rapid, sensitive and reproducible HPLC method was developed and validated for the quantification of Ceritinib in rabbit plasma using PDA detector at wave length 264 nm. The method was developed using Dasatinib as internal standard (IS). Ceritinib is a selective and potent inhibitor of anaplastic lymphoma kinase (ALK) indicated in the treatment of non-small cell lung cancer (NSCLC). The Ceritinib and Dasatinib were separated as symmetrical peaks on an analytical column ODS (250 × 4.6 mm, 5 µm) column using a mixture of 75% phosphate buffer (pH 3.6) and 25% acetonitrile as mobile phase with a flow rate of 1.0 ml/min. The total chromatographic run time is 10.0 min with retention times for Ceritinib and Dasatinib at 7.630 min and 2.771 min respectively, no interferences from the endogenous plasma peaks is observed. The method is validated and linear calibration curves were obtained across a range of 0.002 – 0.2 µg/ml for Ceritinib with a correlation coefficient of 0.999. The coefficients of variation for intra-day and inter-day assays were...
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Posted by admin on Jun 30, 2018 in |
Now a day’s establishing and sustaining competitive advantage depends on the effective development of Knowledge Absorptive Capacity (KAC) and innovations. This study evaluated the role of prior technical experiences in developing firm’s absorptive capacity and the contribution of this type of capacity to the firm’s innovation performance. In this study we designed a questionnaire to assess firms’ KAC and innovation performance. First, the validity and reliability of the questionnaire were assessed. The data were collected from owners/managing directors or research and development managers of 20 Iranian pharmaceutical and biopharmaceutical companies. The following variables were measured: industrial and technical experiences of owners/managing directors and the members of research and development teams, KAC, innovation performance indices and control variables. The questionnaire had appropriate validity and reliability. The findings indicated that the breadth and the depth of the owner’s/managing director’s industrial experiences properly reflected KAC of a pharmaceutical company. In turn, the present research showed that the absorptive capacity of a firm could affect its innovation performance. Organizational strategic regeneration and innovation...
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