Posted by admin on May 31, 2018 in |
The search for natural anti-microbials present in plants has been the target of intense scientific studies over the last years. The objective of this study was to assess the in-vitro antibacterial potential and in silico toxicology of the essential oil of Cinnamomum cassia (Chinese cinnamon) against E. coli and S. aureus strains. The essential oil was initially solubilized in tween 80 and dimethyl sulphoxide acid (DMSO). The antibacterial action was assessed by means of the minimum inhibitory concentration (MIC) determined from the microdilution in double-concentrated brain heart infusion broth (BHI) and the minimum bactericidal concentration (MBC) which was determined by the depletion in nutrient agar (NA) technique with aliquots of 10µL of the MIC, MIC × 2 and MIC × 4. The MIC and the MBC presented satisfactory and varied results; the E. coli LPM 4459, LPM 1243, LPM 4010 and LPM 4896 strains, had a MIC of 256 µg/mL and MBC of 512 µg/mL respectively. As for the E. coli LPM 2810 and S. aureus LPM 55 strains,...
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Posted by admin on May 31, 2018 in |
Chronic stress is a risk factor for the development of many psychopathological conditions in humans, including major depression and anxiety disorders and cognitive dysfunction. It is therefore important to focus on identifying possible mechanism to prevent these stress-induced disorders. Therefore, current study was designed to investigate the effect of Eclipta alba on chronic unpredicted stress (CUS) induced behavioral dysfunction, oxidative stress and neurotransmitter levels in the discrete regions of rat brain. Animals were subjected to acute and chronic unpredictable stress for 30 days, and the effect of Eclipta alba was studied in acute and chronic stressed animals during which 200 mg/kg Eclipta alba was administered orally. Our Results revealed that stressed animals performed poorly in Novel object recognition task (NORT). Eclipta alba alleviated behavioral dysfunction in chronically stressed animals and also significantly increased antioxidant levels such as SOD, Catalase, total thiol and decreased oxidative stress markers such MDA, nitric oxide and hydrogen peroxide in discrete brain regions as compared to CUS. Further increased catecholamine and decreased 5HT due to...
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Posted by admin on May 31, 2018 in |
Acalypha inferno (AI) is a well-known ornamental plant, presently with no known documentation concerning its therapeutic use and safety profile. However, other plants of the same genus, Acalypha, have been reported to have various uses ranging from food to medicine with their related adverse effects. This study therefore focused on the acute and subchronic toxicity effects of aqueous ethanolic leaf extract of Acalypha inferno leaves (AIE) in animals. The acute toxicity study was performed using the fixed dose method. In the subchronic assessment, both male and female rats were separately administered with AIE at doses of 100 mg/kg, 250 mg/kg and 500 mg/kg twice daily for 28 days. Change in body weight, relative organ weight, haematological parameters and biochemical parameters were recorded and analysed. In the acute toxicity study, no deleterious effect was observed up to 5000 mg/kg, hence the LD50≥5000 mg/kg. In the subchronic toxicity study, significant increase in weight was observed at all doses in male rats whilst a significant decrease was observed only at 500 mg/kg in...
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Posted by admin on May 31, 2018 in |
For a formulation scientist, the delivery of therapeutic agents to the diseased organ has been a challenging problem. It is expected to improve the therapeutic index and reduce side effects of a drug by good delivery strategy to diseased organ. The objective of this investigation is to evaluate the targeting potential of solid lipid nanoparticle (SLN) formulation of imatinib mesylate to the lymphatic system using response surface methodology of design of experiment. Box-Behnken DOE was constructed using imatinib mesylate, compritol 888 ATO (X1) and pluronic F68 (X2) as independent factors and particle size (Y1) and entrapment efficiency (Y2) as dependant factors. The SLN formulation was prepared by hot homogenization followed by ultrasonication. Prepared SLN were analyzed by FTIR, DSC, DLS, PXRD and SEM. The optimized particle size and entrapment efficiency of the imatinib mesylate loaded solid lipid nanoparticle was found to be 190 nm and 62.5% respectively, which are sufficient to reach lymphatic system. FTIR revealed no interaction between imatinib mesylate and compritol 888 ATO. Compritol 888 ATO retained...
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Posted by admin on May 31, 2018 in |
Analytical study of Ayurvedic preparations is the need of present scientific era. Though the Ayurvedic drugs are time tested and have been used successfully in the management of various ailments, it is now necessary to prove their quality, efficacy and safety to the scientific world through various modern analytical parameters. Chincha kshara is an important preparation mentioned in ayurvedic classics for the management of various diseases. An attempt has been made in the present study to prepare Chincha kshara and analyse the physical nature, surface morphology and chemical constituents present in the Chincha kshara through analytical parameters like organoleptic properties, pH value, X-ray diffraction technique (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). XRD study of Chincha kshara showed the presence of potassium carbonate hydrate as major and minor peaks and sodium chloride, potassium calcium hydrogen carbonate phosphate hydrate, silver phosphate and magnesium phosphate oxide as minor peaks. SEM revealed its amorphous nature and EDS reported the significant presence of Potassium (50.44%) and Oxygen (46.07%) in...
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