Posted by admin on May 31, 2018 in |
Antioxidants are one of the key troupes in tumorigenesis, several natural and artificial antioxidants were shown to have anticancer effects. The aim of the current study is to reveal the chemopreventive nature of citral during diethylnitrosamine (DEN) -induced liver cancer in male Wistar albino rats. Administration of DEN to rats caused in amplified relative liver weight and serum marker enzymes aspartate transaminase (AST), alkaline phosphatase (ALP), alanine transaminase (ALT), lactate dehydrogenase (LDH) and gamma glutamyltranspeptidase (γGT). The levels of lipid peroxides prominent (in both serum and tissue) with a succeeding reduction in the final body weight and tissue antioxidants like superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx) and glutathione reductase (GR). Citral supplementation (100 mg/kg body weight) knowingly reduced these alterations, thereby showing powerful anticancer effect in liver cancer. Histological interpretations revisions were also conceded out, which supplementary supports to the chemopreventive action of the citral against DEN induction during liver cancer progression. These explanations suggest that citral inhibits lipid peroxidation, hepatic cell damage, and...
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Posted by admin on May 31, 2018 in |
Dithiolphenole (DP) has been synthesized and characterized with IR and NMR spectroscopic methods. A simple and spectrophotometric method has been developed for the determination of trace amounts of vanadium (IV, V). The reagents forms blue coloured insoluble in water complex with vanadium (IV, V) in a weakly acidic and slightly alkaline medium (pH 2.5 – 8.5). In the presence of hydrophobic amines (Am) form ternary complexes. As hydrophobic amine-phenantroline (Phen), batophenantroline (BPhen) and dipiridile (Dip) were used. The molar absorptivity of coloured species are (2.95 – 3.85) × 104 L.mol-1 cm-1. Beer’s law is obeyed in the range 0.2 – 18 μg/ml of vanadium (IV, V) at λmax 610 – 630 nm. Vanadium (IV, V) forms 1: 1: 1 complex with DP and Am, stability constant of the complex was found to be 9.58 – 10.95 (pHopt = 6.4 – 7.9). The proposed method was successfully applied to the analysis of vanadium in soil, natural water samples and plant material. The results obtainned were agreed with the reported methods...
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Posted by admin on May 31, 2018 in |
Artocarpus heterophyllus belongs to the family Moraceae. In Sri Lankan traditional medicine the water extract of A. heterophyllus senescent leaves is used to control diabetes mellitus. The hypoglycemic activity of ethyl acetate fraction of water extract of senescent leaves has been reported. Ethyl acetate fraction (EA/W) was fractionated using sephadex LH-20 column chromatography using the solvent systems: dichloromethane/hexane (4:1), dichloromethane/acetone (3:2), dichloromethane/acetone (1:4), dichloromethane/ methanol (1:1) and methanol to produce five fractions. EA/W and fractions 1-5 were subjected to in-vitro antioxidant, α- glucosidase inhibitory, antiglycation and anti-inflammatory activity studies. Fraction 4 exhibited the highest DPPH radical scavenging activity (IC50 = 21.69 ± 0.31 μg/mL) and α- glucosidase inhibitory activity (IC50 = 0.4± 0.01 μg/ mL) which were greater than the standards gallic acid and acarbose respectively and EA/W. Fractions 3 and 4 showed higher antiglycation activity (IC50 = 0.44 ± 0.04 mg/mL and 0.30 ± 0.01 μg/mL respectively) and higher anti-inflammatory activity (IC50 = 24.4 ± 3.8 and 16.9 ± 0.1 μg/mL respectively) than any other fraction or EA/W. However, these...
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Posted by admin on May 31, 2018 in |
Pogostemon quadrifolius (Benth.) is a medicinal aromatic shrub in family Lamiaceae which is used as folk medicine in India. This present study deals with the preliminary screening of Pogostemon quadrifolius antimicrobial activity and TLC profiling of the crude plant extract. Cold extraction technique was carried out to prepare the crude plant extract, which was further screened for the presence of phytochemicals. The extracts were assessed for antibacterial and antifungal studies by disc diffusion method. Thin layer chromatography was utilized for solvent standardization. Phytochemical analysis revealed the presence of diverse classes of bioactive components. The quantitative analysis of flavanoids, alkaloids and total phenolic content in different extracts varied from each other. Evaluation of antibacterial studies showed that methanolic extract and ethyl acetate extract showed activity against standard and MDR strains respectively. The ethanolic extract also showed fungicidal activity. And finally the solvent standardization was performed for the separation of phyto-constituents by TLC, which showed a very good abundance of active principles (based on their Rf values). All these preliminary reports...
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Posted by admin on May 31, 2018 in |
Screening of medicinal plants for their antimicrobial activity leads to development of less expensive formulations with better safety and efficacy. A. indica and L. camara shows antibacterial activity. A. indica is multipurpose tree with wide range of uses. Also the L. camara has steroidal content showing cardiotonic, antispasmodic activity along with antibacterial activity. In the present study we have compared the individual alcoholic leaf extracts of both plants with their combination. Antibacterial activity in mixture of leaf extracts of Neem (Azadirachta indica) and tantani (Lantana camara) was tested against human pathogenic bacteria E. coli, Staphylococcus aureus, P. aureginosa and Bacillus subtilis. Ethanolic extracts of Neem and Tantani leaves were used. Varying concentrations of individual extracts and their combination of 10 mg/ml, 50 mg/ml, and 100 mg/ml were prepared. Antimicrobial assay using cup plate method was used. When compared with individual leaf extract of Neem and tantani, combination shows maximum inhibition on E. coli, Staphylococcus aureus, Pseudomonas aureginosa, Bacillus subtilis. These good experimental results can further be explored. The mixture...
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