Posted by admin on Jun 30, 2018 in |
Determination of the anti-diabetic drugs vildagliptin and metformin is wildly used to validate drug efficiency in diabetic patients. Here we describe a new and simple chromatographic method to analyze both drugs simultaneously in their commercial pharmaceutical dosage forms. A reverse Phase-high performance liquid chromatography (RP-HPLC) separation method was applied using an Xterra C18 column (250 mmL×4.6 mm I.D × 5µ) with acetonitrile: phosphate buffer (pH 6.0): water (65: 20:15v/v/v) as a mobile phase at a flow rate of 1.0 ml/min. Quantification of these drugs by this method was achieved using an ultra violet detector at λ = 239 nm. The limit of detection (LOD) for vildagliptin was 0.0040 µg/ml and 0.025 µg/ml for metformin using this RP-HPLC method. A linear calibration curves were reached at a concentration range of 4-34 µg/ml and 8-54 µg/ml for vildagliptin and metformin, respectively. The developed technique was validated for concentration linearity, robustness, accuracy and precision, and results were statistically analyzed according to the International Conference on Harmonisation (ICH) guidelines. The results presented in...
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Posted by admin on Jun 30, 2018 in |
The present work includes development of analytical method for simultaneous estimation of Chlorhexidine gluconate and Cetrimide by RP-HPLC method. The chromatographic conditions were successfully optimised for the separation of Chlorhexidine gluconate and Cetrimide by using Hypersil BDS C18 column (4.6 × 150 mm) 5µ, flow rate of 0.8 ml/min, mobile phase ratio of (30:55:15 v/v/v) ACN: methanol: phosphate buffer (KH2PO4 and K2HPO4) phosphate pH 3 (pH was adjusted with orthophosphoric acid) and detection wavelength used was 210 nm. The retention times were found to be 3.10 min and 3.9 min for Cetrimide and Chlorhexidine gluconate respectively. The % purity of Chlorhexidine gluconate and Cetrimide was found to be 99.92% and 100.45% respectively. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1). The linearity study of Chlorhexidine gluconate and Cetrimide was found in concentration range of 3 µg – 18 µg and 30 µg – 180 µg. DOE was applied in validation for checking robustness of the developed method using Box-Behnken design and also normal distribution of...
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Posted by admin on Jun 30, 2018 in |
The present study aimed to investigate the anticancer effect of rosmarinic acid (RA), both alone and in combination with cisplatin (cis). Also, the potential nephroprotective effects of RA against cis-induced nephrotoxicity in mice and the possible mechanisms underlying this protection were explored. Treatment of HepG2 cells with RA produced a significant decline in cellular proliferation. RA induced cell cycle arrest at G0 / G1 phase and S phase, significantly inhibited vascular endothelial growth factor (VEGF) concentration and induced caspase-3 level in cell lysate. While pre-treatment with IC50 RA before cis addition significantly enhanced cis cytotoxic activity in HepG2 cells. Moreover, Pre-treatment with RA significantly mitigated cis-induced elevation of blood urea nitrogen (BUN) and serum creatinine levels. The elevated levels of malondialdehyde (MDA), tumor necrosis factor- alpha (TNF-α), Bax and caspase-9 in kidney tissues were significantly reversed. Additionally, RA significantly abrogated cis-induced reduction in reduced glutathione level (GSH). The histopathological examination emphasized the obtained results. Conclusion: RA possesses cytotoxic activity against HepG2 cell line through cell cycle arrest, induction of...
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Posted by admin on Jun 30, 2018 in |
Polyherbal preparations (PHP) are known to express high effectiveness in a vast number of diseases. In view of this, present investigation was carried out to identify phytochemical constituents of PHP, prepared from the aqueous extract of test plant powders of Mimusops elengi Linn., Strobilanthes barbatus Nees, Indigofera zollingeriana Miquel and Dillenia indica Linn. Phytochemical screening of APHP revealed the presence of carbohydrates, Proteins and amino acids, alkaloids, glycosides, phenols, flavonoids, and fixed oils/fats. Acute toxicity studies were conducted on female wister rats. Study design is according to OECD Guidelines no. 423. APHP was administered at dose of 5 mg/kg, 50 mg/kg, 250 mg/kg, 500 mg/kg, 1000 mg/kg and 2000 mg/kg body weight as suspension (1% Na CMC) along with blank. No-Observed-Adverse-Effect- Level (NOAEL) of APHP was found to be 2000 mg/kg. In connection to that, sub chronic toxicity studies have been carried out to predict the dose to be selected for in-vitro and in-vivo experiments. APHP at the doses of 250 mg/kg, 500mg/kg and 1000mg/kg were administered and examined...
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Posted by admin on Jun 30, 2018 in |
Diethylstilbestrol (DES) is an endocrine disturbing compound it can mimic and perform like a natural hormone, which mimics estradiol by binding to the estrogen receptor. This compound mainly reproductive target, so in this study used to induce endometriosis. Endometriosis is an estrone dependent constant gynaecological disorder. Here it, intends to induce endometriosis and treated by the MMP inhibitor to check whether it could attain the implantation or not. Hence the present study induces endometriosis by three different concentrations such as 10, 15 and 20 µg/kg body weight for 20 days at alternatively while to check the estrous cycle, hormonal regulation at 7th, 15th and 20th day also to record the body weight and reproductive organ weight from initial to final day of DES induction. Endometrial lesion developments were confirmed by anatomical observation. The above result reveals 20 µg DES induced group leads to endometrial cancer, so other 10 & 15 µg DES induced group carry out to further analysis. The endometrial lesions were treated by 10 µM concentrations of...
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