Posted by admin on May 31, 2018 in |
Dithiolphenole (DP) has been synthesized and characterized with IR and NMR spectroscopic methods. A simple and spectrophotometric method has been developed for the determination of trace amounts of vanadium (IV, V). The reagents forms blue coloured insoluble in water complex with vanadium (IV, V) in a weakly acidic and slightly alkaline medium (pH 2.5 – 8.5). In the presence of hydrophobic amines (Am) form ternary complexes. As hydrophobic amine-phenantroline (Phen), batophenantroline (BPhen) and dipiridile (Dip) were used. The molar absorptivity of coloured species are (2.95 – 3.85) × 104 L.mol-1 cm-1. Beer’s law is obeyed in the range 0.2 – 18 μg/ml of vanadium (IV, V) at λmax 610 – 630 nm. Vanadium (IV, V) forms 1: 1: 1 complex with DP and Am, stability constant of the complex was found to be 9.58 – 10.95 (pHopt = 6.4 – 7.9). The proposed method was successfully applied to the analysis of vanadium in soil, natural water samples and plant material. The results obtainned were agreed with the reported methods...
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Posted by admin on May 31, 2018 in |
Artocarpus heterophyllus belongs to the family Moraceae. In Sri Lankan traditional medicine the water extract of A. heterophyllus senescent leaves is used to control diabetes mellitus. The hypoglycemic activity of ethyl acetate fraction of water extract of senescent leaves has been reported. Ethyl acetate fraction (EA/W) was fractionated using sephadex LH-20 column chromatography using the solvent systems: dichloromethane/hexane (4:1), dichloromethane/acetone (3:2), dichloromethane/acetone (1:4), dichloromethane/ methanol (1:1) and methanol to produce five fractions. EA/W and fractions 1-5 were subjected to in-vitro antioxidant, α- glucosidase inhibitory, antiglycation and anti-inflammatory activity studies. Fraction 4 exhibited the highest DPPH radical scavenging activity (IC50 = 21.69 ± 0.31 μg/mL) and α- glucosidase inhibitory activity (IC50 = 0.4± 0.01 μg/ mL) which were greater than the standards gallic acid and acarbose respectively and EA/W. Fractions 3 and 4 showed higher antiglycation activity (IC50 = 0.44 ± 0.04 mg/mL and 0.30 ± 0.01 μg/mL respectively) and higher anti-inflammatory activity (IC50 = 24.4 ± 3.8 and 16.9 ± 0.1 μg/mL respectively) than any other fraction or EA/W. However, these...
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Posted by admin on May 31, 2018 in |
Pogostemon quadrifolius (Benth.) is a medicinal aromatic shrub in family Lamiaceae which is used as folk medicine in India. This present study deals with the preliminary screening of Pogostemon quadrifolius antimicrobial activity and TLC profiling of the crude plant extract. Cold extraction technique was carried out to prepare the crude plant extract, which was further screened for the presence of phytochemicals. The extracts were assessed for antibacterial and antifungal studies by disc diffusion method. Thin layer chromatography was utilized for solvent standardization. Phytochemical analysis revealed the presence of diverse classes of bioactive components. The quantitative analysis of flavanoids, alkaloids and total phenolic content in different extracts varied from each other. Evaluation of antibacterial studies showed that methanolic extract and ethyl acetate extract showed activity against standard and MDR strains respectively. The ethanolic extract also showed fungicidal activity. And finally the solvent standardization was performed for the separation of phyto-constituents by TLC, which showed a very good abundance of active principles (based on their Rf values). All these preliminary reports...
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Posted by admin on May 31, 2018 in |
Screening of medicinal plants for their antimicrobial activity leads to development of less expensive formulations with better safety and efficacy. A. indica and L. camara shows antibacterial activity. A. indica is multipurpose tree with wide range of uses. Also the L. camara has steroidal content showing cardiotonic, antispasmodic activity along with antibacterial activity. In the present study we have compared the individual alcoholic leaf extracts of both plants with their combination. Antibacterial activity in mixture of leaf extracts of Neem (Azadirachta indica) and tantani (Lantana camara) was tested against human pathogenic bacteria E. coli, Staphylococcus aureus, P. aureginosa and Bacillus subtilis. Ethanolic extracts of Neem and Tantani leaves were used. Varying concentrations of individual extracts and their combination of 10 mg/ml, 50 mg/ml, and 100 mg/ml were prepared. Antimicrobial assay using cup plate method was used. When compared with individual leaf extract of Neem and tantani, combination shows maximum inhibition on E. coli, Staphylococcus aureus, Pseudomonas aureginosa, Bacillus subtilis. These good experimental results can further be explored. The mixture...
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Posted by admin on May 31, 2018 in |
Introduction: There are numerous animal models available for polycystic ovary syndrome (PCOS), a fully convincing model for PCOS reflecting all pathologic condition of PCOS seen in humans is not available due to difficulties in inducing all pathologies in rat models. If so, it will help to test drug to treat PCOS. The main goal of this study is to create a new model to reflect all pathologic conditions of PCOS seen in humans using non-steroidal aromatase inhibitor letrozole and fructose in rats. Methods: Twelve rats were divided into two groups containing 6 rats each. Vehicle control group rats received 1% aqueous solution of carboxmethlycellulose (CMC) once daily orally for 28 days and PCOS group was administered letrozole at a concentration of 1 mg/kg dissolved in 1% CMC p.o. once daily for 28 days. Along with these rats were allowed free access of 10% fructose solution daily. During experimental period, vaginal smears were collected daily for estrus cycle determination. Rats were sacrificed and blood sample were collected for hormonal and...
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