Posted by admin on Jun 30, 2018 in |
Cancer, the uncontrolled growth of cells, has been estimated as a major cause of death among human population. Although there are many potential drugs, a much effective bioactive drug with less side effects are still in its pioneer stage of research. The aim of the study was to isolate, purify and characterize the anticancer muscle protein from edible marine catfish, Tachysaurus dussumieri, so as to project it as an alternative medicine for cancer. The anticancer muscle protein was purified to its homogeneity from the edible marine catfish Tachysurus dussumieri through ammonium sulphate fractionation and ion exchange chromatography. Both partially purified crude fractions and isolated purified muscle protein were screened for its anticancer activity by MTT assay. The fraction III revealed prominent anti-cancer activity which was subjected to MALDI-TOF MS analysis for its molecular weight confirmation. Further, Peptide Mass Finger printing analysis (PMF) was executed to reveal its nature. To reconfirm its anticancer action, DAPI, PI staining and Comet assay were executed. The purified dimer having 13.285 kDa weight was...
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Posted by admin on Jun 30, 2018 in |
A rapid, sensitive and reproducible HPLC method was developed and validated for the quantification of Ceritinib in rabbit plasma using PDA detector at wave length 264 nm. The method was developed using Dasatinib as internal standard (IS). Ceritinib is a selective and potent inhibitor of anaplastic lymphoma kinase (ALK) indicated in the treatment of non-small cell lung cancer (NSCLC). The Ceritinib and Dasatinib were separated as symmetrical peaks on an analytical column ODS (250 × 4.6 mm, 5 µm) column using a mixture of 75% phosphate buffer (pH 3.6) and 25% acetonitrile as mobile phase with a flow rate of 1.0 ml/min. The total chromatographic run time is 10.0 min with retention times for Ceritinib and Dasatinib at 7.630 min and 2.771 min respectively, no interferences from the endogenous plasma peaks is observed. The method is validated and linear calibration curves were obtained across a range of 0.002 – 0.2 µg/ml for Ceritinib with a correlation coefficient of 0.999. The coefficients of variation for intra-day and inter-day assays were...
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Posted by admin on Jun 30, 2018 in |
Now a day’s establishing and sustaining competitive advantage depends on the effective development of Knowledge Absorptive Capacity (KAC) and innovations. This study evaluated the role of prior technical experiences in developing firm’s absorptive capacity and the contribution of this type of capacity to the firm’s innovation performance. In this study we designed a questionnaire to assess firms’ KAC and innovation performance. First, the validity and reliability of the questionnaire were assessed. The data were collected from owners/managing directors or research and development managers of 20 Iranian pharmaceutical and biopharmaceutical companies. The following variables were measured: industrial and technical experiences of owners/managing directors and the members of research and development teams, KAC, innovation performance indices and control variables. The questionnaire had appropriate validity and reliability. The findings indicated that the breadth and the depth of the owner’s/managing director’s industrial experiences properly reflected KAC of a pharmaceutical company. In turn, the present research showed that the absorptive capacity of a firm could affect its innovation performance. Organizational strategic regeneration and innovation...
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Posted by admin on Jun 30, 2018 in |
The present work was planned to study the effect of methyl parathion an organophosphate compound (OP) on biochemical contents of fresh water cat fish Heteropneustis fossilis. The exposure of fish to the sub-lethal concentrations of the toxicant methyl parathion was investigated and the variations were observed in biochemical contents in different tissues of the fish i.e. gill, liver, intestine, muscle and brain. The quantitative variations were observed in proteins, carbohydrates and ninhydrine positive substances at different time intervals i.e. 24, 48, 72 and 96 h. The results revealed that the components of proteins, carbohydrates and ninhydrine positive substances were found to be decreased significantly at different time intervals of methyl parathion exposure to different tissues of fish compared to control. The maximum decrease in proteins followed by ninhydrine positive substances (free amino acids) and carbohydrates was observed at 72 h and 96 h compared to 24 h and 48 h in different tissues of the fish H. fossils. Thus, our present investigation reports that the changes observed were depending...
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Posted by admin on Jun 30, 2018 in |
A High-Performance Liquid Chromatography (HPLC) technique has been developed for the determining of foreign impurities in the parent substance of carboren. We have proposed the most sensitive, selective and also the universal HPLC method to be used, which would allow to conduct the qualitative and quantitative analysis under the same conditions so that to determine foreign impurities in the substance. Reliable separation of carboren and impurities was performed on a liquid chromatography equipped with a spectrophotometric detector and an analytical stainless steel column (250 × 4, 6 mm) of size, filled with C18 sorbent (5m km), in gradient elution mode. The detection of limits for carboren and identified of potential organic impurities were determined. It was found that the content of any other individual impurity did not exceed 0.1%, the total content of impurities did not exceed 0.5%. In the samples, impurities A, B and C were detected with a relative retention time of 0.35; 0.42 and 0.30. The results were repeatedly reproduced in model mixtures, parent substance...
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