Posted by admin on May 31, 2018 in |
Cefepime is a broad – spectrum new parenteral cephalosporin used to treat moderate to severe nosocomial pneumonia, empirical treatment of febrile neutropenia and infections of the skin and urinary tract. In this present study a simple, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for analysis of cefepime powder for injectable solutions along with its potency by using microbiological bioassay by four different microorganisms. A YMC C 18 (4.6 × 150 mm, 5.0 mm) column was used for cefepime separation, using isocratic elution with acetonitrile: water (70:30, v/v) and UV detection at 235 nm. Microbiological assay (bioassay) was performed using the agar diffusion method. The validation performed yielded good results in terms of linearity, precision, accuracy, and robustness. The retention time obtained for cefepime was 1.77 min and % potency of the marketed dosage form was found to be 147.9%, 100%, 83.17% and 125.8%...
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Posted by admin on May 31, 2018 in |
The objective of this work was improvement in the ocular bioavailability of diclofenac sodium by enhancing drug permeation across cornea and promoting drug retention time on ocular surface. Using pseudo ternary phase diagrams transparent regions were identified and nanoemulsions were prepared by self-emulsification method, which were further optimized by emulsification study and drug content. Three best NE formulations were incorporated in 20% poloxamer 407 (thermosensitive polymer solution) to formulate nanoemulsion embedded thermosensitive in-situ ocular gel and further evaluated on the basis of gelation temperature and drug entrapment. Optimized nanoemulsion formulation was evaluated by TEM, particle size analysis and zeta potential. The optimized NE gel formulation changed from sol-gel phase at physiological temperature. Comparison of dissolution profile of developed formulation was carried out with the marketed formulation. The formulated NE in-situ gel showed drug release for a longer duration of time as compared to the marketed eye drops (0.1% w/v Voltaren Ophtha). The in-vitro release data was fitted to various kinetic models. It was found that the in-vitro drug release...
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Posted by admin on May 31, 2018 in |
Anti-diabetic and protective activity of T. dioica aqueous extract in diabetic complications in streptozotocin (STZ)-induced diabetic rats was evaluated. Albino rats (n = 24) were divided into four groups, of six animals each. Group 1 (normal control) and Group 2 (diabetic control) received normal saline (10 ml/kg/day p.o.) whereas Group 3 (Standard, insulin 6 U/kg/day s.c) and Group 4 received 1000 mg/kg/day p.o. of T. dioica extract for 28 days. Extract treated animals showed significant decrease in various biochemical parameters like blood glucose, SGOT, SGPT, creatinine, urea, LDL, Cholesterol and glycated hemoglobin. Extract was also effective in diabetic complications like neuropathy and learning and memory dysfunction evaluated by behavioral models like elevated plus maze, object recognition, open field test, rotarod, hot plate, tail flick, immersion in hot and cold water etc. It can be concluded that T. dioica possess significant anti-diabetic and protective activity in diabetic complications in STZ-induced diabetic...
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Posted by admin on May 31, 2018 in |
Bacterial biofilms due to antibacterial resistance are causing serious health issues with respect to infection control, its prognosis and treatment of disease. The current scenario demands need of new and alternative antibacterial compounds for treatment of such infections. The current study highlights the use of polluted river, Waldhuni, as a source of potent microorganisms with antibiofilm properties. 186 microorganisms were isolated from Waldhuni River, and tested for quorum sensing inhibitory activity (QSI) against C. violaceum MTCC 2656. Seven isolates exhibited QSI activity, out of which 1 isolate PB2 was selected for further study based on its high QSI potential. PB2 displayed broad spectrum antibiofilm activity against eight strains of biofilm forming pathogens. The effective minimum biofilm eradication concentration (MBEC) of crude extract of PB2 secondary metabolite (SME) was found to be 500ppm. The extract reduced exo-polysaccharide (EPS) by 94.16%, viability by 90% and biofilm density by 96% in 6h treatment. It also rendered to inhibit extracellular DNA of Streptococcus mutans MTCC497, Enterococcus faecalis MTCC 439 and Pseudomonas aeruginosa MTCC...
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Posted by admin on May 31, 2018 in |
Rapid, sensitive, specific and validated colorimetric methods have been developed for the quantitative estimation of ambroxol HCl in bulk and dosage form. The current method was developed based on oxidation of ambroxol with Gibb’s reagent (method I) and p-diethyl amino benzaldehyde in toluene (method II). The formed intense colour complex was measured at 537.2 nm and 438.4 nm respectively. Under optimized experimental conditions, Beer’s law is obeyed in concentration range 5 – 30 µg/ml for both the methods with regression co-efficient 0.9994 and 0.9992. Recovery studies were conducted by standard addition method to confirm the accuracy of the method. The LOD and LOQ for the estimation of ambroxol were found as 0.0773, 0.2343 for method I and 0.0667, 0.2021 for method II respectively. The proposed method was validated as per ICH...
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