Posted by admin on Apr 30, 2018 in |
Commercially available two polyherbal formulations (Chandra Kalka and Sharkaradi Kalka) were collected from local market. The objective of this study was to determine the Total Phenolic Content (TPC) , Total Flavonoid Content (TFC) of these two ‘Kalkas’ and to investigate the existence of antioxidant, anti-inflammatory, antibacterial and anti-tyrosinase activities. Total phenolic content and total flavonoid content was determined by Folin Ciocalteu and Aluminium chloride method respectively. Antioxidant activities was evaluated using the DPPH radical scavenging assay and human red blood cell membrane stabilization assay was used to determine the anti-inflammatory activity. Agar well diffusion method and tyrosinase enzyme inhibitory assay was facilitated to investigate the antibacterial and tyrosinase enzyme inhibition properties of the selected polyherbal formulations, accordingly. The results obtained from the study showed that Chandra Kalka has the highest TPC (53.90 ± 948 mg GAE /g extract) and has the highest TFC (119.25 ± 10.11 mg QE/g extract). Sharkaradi Kalka showed higher antioxidant activity for the DPPH radical scavenging, anti-inflammatory activity, and anti-tyrosinase activity for the assays conducted,...
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Posted by admin on Apr 30, 2018 in |
A new, simple, sensitive, precise, accurate and stability indicating RP-HPLC method has been developed for the simultaneous estimation of salbutamol and beclomethasone in combined tablet dosage form. The analysis was carried out at 230 nm and this chromatographic separation was achieved with Kromasil 250 C18 [250 x 4.6 mm, 5µ] column under 25 oC temperature and using mobile phase water and acetonitrile in a ratio of 55:45 v/v adjusted pH 2 with ortho phosphoric acid. The retention times of salbutamol and beclomethasone were found to be 2.030 ± 0.01 min, 3.271 ± 0.1min respectively. The proposed method was validated according to ICH guidelines. The linearity study of salbutamol and beclomethasone was found in concentration range of 25 – 150 µg/ml and 50 – 300 µg/ml and correlation coefficient (r2) was found to be 0.999 and 0.999. The percentage recovery was obtained as 98.61 % and 100.75 % w/v for beclomethasone and salbutamol. The LOD, LOQ values were obtained from regression equations of beclomethasone and salbutamol were 1.13 µg/ml, 2.06...
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Posted by admin on Apr 30, 2018 in |
The present study was undertaken to investigate some organic phytochemicals, fatty acids, toxic metals and evaluate lipidemic and antidiabetic activity of a herbal medicine and its one constituent Syzygium cumini seed. Antidiabetic and lipidemic activity of this medicine and seed extract was studied on streptozotocin induced type 2 diabetic model rats. From chemical investigation carbohydrates and steroids were present in both samples. Alkaloid was present in herbal medicine sample only. Arsenic was found to be 0.05 ppm which was less than the tolerance level. Other toxic metals Lead, Copper, Cadmium and Manganese were absent in herbal medicine (HM-01). Myristic, palmotelic, palmitic, linoleic, oleic, stearic and arachidic acids were found in both samples. The rats were divided into 4 groups (n = 6 in each group): a) Water control b) Glibenclamide c) PESE d) HM-01. The parameters measured were: serum glucose and serum lipid profile. Oral consumption of HM-01 and PESE for 22 days caused significant (plasma glucose in mmol/L, Mean ± SD; 8.57± 1.09 vs 5.67 ± 0.45; and...
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Posted by admin on Apr 30, 2018 in |
Hydrocotyle sibthorpiodes is a known medicinal plant and is used for treatment against many bacterial and fungal pathogens in a conventional application form. The present study was intended to enhance the activity of H. sibthorpiodes plant extract against four bacterial species in combination with clove oil and silver nanoparticles by preparing a chitosan based nanocapsule that cause chronicity in wounds. The size of the synthesized nanocapsules was studied by Dynamic Light Scattering study (DLS) and SEM and was found to be 138.2 d.nm. However, the size of the loaded nanocapsule without AgNPs was obtained with a reduced size of 124.8 d.nm. The analysis of the result for the antibacterial study of the plant extract loaded nanocapsule with AgNPs was also found to be more than the extract alone and nanocapsules without AgNPs with an inhibition zone of 3.9 cm and 3.2 cm against both S. aureus as well as K. pneumonia respectively which was considered to be more effective than the conventional application of the plant extract for inhibiting...
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Posted by admin on Apr 30, 2018 in |
This work aimed to evaluate the antidiarrheal effect of aqueous extract of steam barks of C. pentandra. Diarrhoea was induced by intragastric administration of castor oil. Intestinal transit was evaluated by using the charcoal method. The effect of the aqueous extract on muscarinics and α-adrenergic receptors was evaluated in atropine and yohimbine presence by using charcoal method. The results obtained show that the aqueous extract (400 and 800 mg/kg) inhibits the appearance of the signs of diarrhoea and increase the onset of gastric evacuation compared to the group control. In addition, yohimbine partially raises antidiarrheal effect observed with aqueous extract at dose of 800 mg/kg, whereas atropine potentiates it. These results are in favor of an antidiarrheal...
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