Posted by admin on Feb 28, 2018 in |
Ephedra gerardiana is an important medicinal plant used as Somalata in Indian Ayurvedic system of medicine and as traditional Chinese medicine since several thousand years. Plant is known to contain valuable secondary metabolite such as ephedrine and pseudo ephedrine. These secondary metabolites are utilized for several medicinal purposes. The present study describes the in vitro regeneration of Ephedra gerardiana using tissue culture technique. Experiments were conducted to study the effect of various combinations of auxins (IBA) and cytokinins (BAP) alone or in different combinations on in vitro culture of nodal segments. Shoot induction and elongation from cultured nodal segments were obtained onto MS medium supplemented with various concentrations of BAP and 15 µM Kinetin. MS+IBA media was utilized for in vitro root induction. Among different media combinations utilized MS+ 5 µM BAP+ 15 µM kinetin was found best for shooting and ¼ MS+ 20 µM IBA was most appropriate for...
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Posted by admin on Feb 28, 2018 in |
A spectrophotometric and reverse phase high performance liquid chromatography methods has been developed for the estimation of Ranolazine in bulk and pharmaceutical dosage form. For UV spectrophotometric method Ranolazine shows maximum absorbance at 273 nm with the concentration ranges of 20-150 µg/mL. The detection limit and quantification limit were found to be 1.23 and 5.21 µg/mL, respectively. Accuracy was in the range of 99.8-100.2%. Intraday and interday precision was in the range 0.16- 0.38% and 0.50-0.82% respectively. The RP-HPLC separation was achieved by using Phenomenex Gemini C18 (100mm X 4.6mm), 3μm column and buffer: acetonitrile (60:40, v/v), (1.0 mL of triethylamine in 1000 mL milli- Q water, pH adjusted to 6 using dilute o-phosphoric acid solution) as mobile phase. The flow rate was 1.0 mL/min and effluents were monitored at 224 nm. The retention time was 5.08 min. The linearity was in the range of 20-150 µg/mL. The detection limit and quantification limit were found to be 1 and 4 µg/mL, respectively. Accuracy was in the range of 99.9-100.1%....
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Posted by admin on Feb 28, 2018 in |
The Pharmacological effects of silver nanoparaticle of Stemodia viscosa aqueous plant extract on albino wistar rats was explored. The plant extract 40 ml was added to 10 ml of 10mM silver nitrate aqueous solution and kept at room temperature for synthesizing silver nanoparaticle. Diabetes was induced in overnight fasted mice by single intra-peritoneal injection of 55 mg/kg of Streptozotozin (STZ), freshly dissolved in 0.1M cold citrate buffer, pH 4.5. After five days of STZ administration, blood was collected and plasma glucose levels were determined. For wound healing activity, a control ointment base was formulated without any drug content. Two creams were formulated by using 5% extract were incorporated in 100gm of cream base. The observation of the percentage wound contraction were made on 4th , 8th , 12th , 16th and 22nd day of post wounding days. All the values were statistically analyzed by unpaired student test comparing with control. The administrations of plant extract to STZ induce diabetic mice, significantly lowered the plasma glucose level as compared to...
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Posted by admin on Feb 28, 2018 in |
Benzimidazole is a heterocyclic aromatic organic compound which has been an important pharmacophore and privileged structure in medicinal chemistry. Substituted benzimidazoles have considerable interest as compounds with a wide spectrum of biological activity and low toxicity. In the present study, a new series of benzimidazole thiourea derivatives are synthesize and screened for antibacterial and antifungal activity. The Methyl 4-(1H-benzo[d]imidazol-2-yl) phenyl carbamodithioate amine derived from Methyl 4-(1H-benzo[d]imidazol-2-yl) phenyl carbamodithioate (TD1-TD6) have been synthesized by reacting the thio methyl group with different amines in presence of ethanol. The synthesized compounds were characterized by FT-IR, 1H NMR, Mass spectra and elemental analysis. The synthesized compounds were screened for their in vitro growth inhibiting activity against four bacterial strains Bacillus subtilis, Staphylococcus aureus (Gram positive bacteria); Escherichia coli, Pseudomonas aureginosa (Gram negative bacteria) and two fungal strains namely Candida albicans and Asperginous niger. The synthesize compounds were compared with standard agents norfloxacin for antibacterial and fluconazole for antifungal activity using well plate method. Compounds exhibit good activity in bacteria Bacillus subtilis and fungous...
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Posted by admin on Feb 28, 2018 in |
An ethanolic extract of Kariyat (Andrographis paniculata) was prepared by using Soxhlet extractor. Kariyat ethanolic extract (KEE) was subjected for phytochemical investigations and screened for analgesic and antipyretic activities in experimental animals. High performance thin layer chromatography (HPTLC) and gas chromatography mass spectrometry (GC-MS) investigations indicated presence of steroids, phenols, terpenoids, alkaloids, saponins, flavonoids etc. The various concentrations ethanolic extract were tested for analgesic activity in mice by using acetic acid-induced writhing in mice and hot-plate method. The maximum protection of 56.23 % against writhing movement was observed at 300mg/kg when compared to standard drug which showed 79.43% protection. Analgesic activity of KEE using hot plate method indicates mean basal reaction time increased significantly in the treated group compared to control group from 30 min onwards. The various concentrations of KEE also tested for antipyretic activity in rats by using Brewer’s Yeast Induced Hyperpyrexia and results significant antipyretic activity 1 hr after drug administration. Mechanism of action was determined by studying prostaglandin inhibition studies. Results indicated that KEE produces...
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