Posted by admin on Feb 28, 2018 in |
In this study, the stomach environment of paracetamol and metaclopramide active substances was prepared with the aid of chemometric method and UV spectrophotometer and their concentrations were measured at certain ratios. Spectra of these two active substances were observed separately in UV spectroscopy at different concentrations. Spectra of paracetamol and metaclopromide were observed by UV spectroscopy at certain ratios and concentrations. These observed spectra gave us an idea by using the chemometric method to generate numerical data indicating whether these two drugs should be used together. Absorbance and concentration values were used in Minitab and other chemometric programs to calculate estimated concentrations with PCR and PLS. In the first step, the synthetic mixtures including paracetamol and metaclopromide were prepared and absorbance values are obtained from spectrophotometry. The second step, in drug tablets (Parol and Metpamid) was calculated amounts for paracetamol and metaclopromide. Because of recovery and standard deviation were accomplished, this study encouraged us to applied for drug...
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Posted by admin on Feb 28, 2018 in |
Andrographolide (AG) is a purified isolate of chemical synthesis resulted from sambiloto herbs (Andrographis paniculata Nees.), which has various pharmacological actions such as anti-inflammatory, hepatoprotector, antidiabetic, inhibition of replication of the HIV virus, antimalarial, anti-arthritic, anti-hyperlipidemic, anticancer, antimicrobial, immunomodulator and antiparasitic. To enhance the ability of AG to penetrate the membrane on transdermal administration, it is important to conduct the formation of inclusion complexes using beta cyclodextrin (BCD) because it has benefit such as enhancing drug release and/or permeation and stabilizing drugs in the formulation or at the absorptive site. The inclusion complexes of AG with BCD was prepared by a solvent evaporation method with mole ratio of 1:1, 1:2 and 2:1. The solids of AG inclusion complex resulted in the BCD were characterized using physical characterization of X-ray diffraction, differential scanning calorimetry, scanning electron microscope, compared to the single compound of AG, BCD, and a physical mixture of AG-BCD. Based on the results of physical characterization, the ratio that indicates the formation of inclusion complexes AG-BCD is the...
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Posted by admin on Feb 28, 2018 in |
Mercury is a hazardous, environmental and industrial pollutant, and causes several health issues related to various organs such as brain, kidney, gut, liver. In 2013, Environmental Protection Agency, USA, reported a higher concentration of mercury (5.8 µg/L) in approximately 1.4 million women (2.3% of total USA population) of reproductive age. Based on this prevalence, it was estimated that more than 75,000 newborns would have increased risk of learning disabilities associated with in-utero exposure to methylmercury annually. Curcumin, an active compound of turmeric, is well known for its antioxidant property and used in various diseases including inflammation and cancer. Here, we have shown that curcumin administration decreases mercury accumulation in liver and kidneys, and which leads to suppression of mercury toxicity. For the purpose, experimental rats were administered mercury chloride with and without curcumin treatment in time dependent manner. At the end of the study, we observed that the accumulation of mercury was higher into liver than kidneys measured using ICP-AES. Curcumin treatment significantly reduces mercury accumulation up to 58...
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Posted by admin on Feb 28, 2018 in |
Seaweeds form economically important component of marine ecosystems worldwide. A wide range of beneficial and detrimental interactions exists between seaweeds and epiphytic as well as endophytic bacteria. Diversity study can be used to retrieve ecological information about community partner that relates the degree of stability of that community. The present work deals with the study the microbial diversity of Ulva lactuca. Epiphytic and endophytic bacteria were isolated from U. lactuca. Total of 4 bacterial isolates were obtained from this seaweed. Among them 2 (VP2 and VP4) were endophytic bacteria and 2 were epiphytic bacteria (VP6 and VP7). Gram staining and biochemical tests for identification of micro-organisms were performed. On the basis of colony morphology and biochemical tests, VP2, VP4 and VP6 were identified as Enterobacter aerogenes, Bacillus cereus and Staphylococcus aureus respectively. Out of 4, 1 isolate could not be identified on the basis of biochemical tests performed. Hence, molecular characterization was attempted by targeting 16S rDNA sequencing for the fourth isolate, VP7. The amplified product was identified as...
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Posted by admin on Feb 28, 2018 in |
The dramatic increase in the burden of chronic non-communicable diseases (NCDs) in emerging economies has become a huge challenge and as such led to increased reliance on folkloric medicine for NCDs management. Consequently, wild Olea europaea leaves were assessed for medicinal potentials, as they are commonly used as a traditional therapy for these diseases in Eastern Cape, South Africa. The antioxidant and antibacterial properties of the O. europaea extracts obtained using different solvents by maceration method were tested by spectrophotometric, disc diffusion and agar dilution methods respectively, while the essential oil obtained in a Clevenger modified apparatus was analyzed by GC-MS. The O. europaea extracts showed moderate to strong inhibitory potential in scavenging the three different tested radicals in a concentration dependant-manner. The ethyl acetate leaf extract (MEA) and ethanol extract (ME) were active against the growth of the six tested bacteria in particular Klebsiella pneumoniae and Serratia marcescens. Gas chromatography – mass spectroscopy studies on the essential oil revealed 61 compounds, accounting for 93.031% of the total oil....
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