Posted by admin on Apr 30, 2018 in |
Inflammatory bowel disease (IBD) is a multifactorial process that causes ulcerative colitis and Crohn’s disease due to unknown mechanisms associated with release of proinflammatory mediators. Aim of the present study is to evaluate inhibitory activity by bark extract Tectona grandis Linn. (TGE) against experimental induced IBD. Two different models were used to induce IBD viz., Indomethacin-induced enterocolitis and acetic acid induced ulcerative colitis in rats. Male wistar rats were pretreated with TGE the dose of 10 and 25 mg/kg p.o. daily for a period of 7 days. On 8th and 9th day indomethacin was administered (7.5 mg/kg, s.c.) for to induce enterocolitis. In case of acetic acid induced ulcerative colitis model, two ml (3%, v/v) acetic acid in saline was instilled into the rectum of a rat. Quantification of inflammation was done using myeloperoxidase assay (MPO), lactate dehydrogenase (LDH), lipid peroxidase (LPO). All parameters were altered in ulcerated rats, and improved in animals receiving TGE an effect that was comparable to that of the standard sulfasalazine, especially at the...
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Posted by admin on Apr 30, 2018 in |
The aim of the present work is to develop and evaluate colon targeted drug delivery of satranidazole for an effective and safe therapy of amoebiasis. Amoebiasis is a parasitic disease caused by E. histolytica and it causes about 34 – 50 million symptomatic infections each year. The treatment of amoebiasis is effective with satranidazole. Therefore it was planned to develop a suitable dosage form, which can deliver maximum amount of the drug in the colonic environment such that there would be effective action on amoebiasis. This investigation was planned to characterize the satranidazole for their physico-chemical properties and to evaluate rheological and compressional characteristics of the tablets. It was planned to optimize the amount of PH dependent polymer eudragit L100 and eudragit S100 carriers for colonic delivery. The coating formulation consisted of Eudragit L100: Eudragit S100 in ratio of 6:4 at 10% coating level has potential for the required lag time of 5 hours. In-vitro release study was shown after effective lag...
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Posted by admin on Apr 30, 2018 in |
Bioactive compounds are the frontline potent agents in both nutraceutical and pharmaceutical industries. Recently, the bioactive compounds are gaining much importance for their ability in enhancing resistance to various diseases and to improve the health of people both by traditional and modern ways of administrations. Therefore, in the present investigation was undertaken to identify the compounds present in the leaf of Waltheria indica Linn. by Gas Chromatography-Mass Spectrometry (GC-MS) analysis by ethanol extract. The GC-MS analysis showed 27 bioactive compounds. The major compounds identified are 2,3-Dihydro-3,5-Dihydroxy-6-methyl-4h-pyran-4-one, tetradecane, nonadecane, tetracosane, phytol and squalene. In a mass spectrum, each compounds were identified based on their retention time and peak area. Pharmacological activities of these compounds indicated that the compounds present in the leaf of said plant can be used as a crude drug and can also be used to develop a novel...
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Posted by admin on Apr 30, 2018 in |
Commercially available two polyherbal formulations (Chandra Kalka and Sharkaradi Kalka) were collected from local market. The objective of this study was to determine the Total Phenolic Content (TPC) , Total Flavonoid Content (TFC) of these two ‘Kalkas’ and to investigate the existence of antioxidant, anti-inflammatory, antibacterial and anti-tyrosinase activities. Total phenolic content and total flavonoid content was determined by Folin Ciocalteu and Aluminium chloride method respectively. Antioxidant activities was evaluated using the DPPH radical scavenging assay and human red blood cell membrane stabilization assay was used to determine the anti-inflammatory activity. Agar well diffusion method and tyrosinase enzyme inhibitory assay was facilitated to investigate the antibacterial and tyrosinase enzyme inhibition properties of the selected polyherbal formulations, accordingly. The results obtained from the study showed that Chandra Kalka has the highest TPC (53.90 ± 948 mg GAE /g extract) and has the highest TFC (119.25 ± 10.11 mg QE/g extract). Sharkaradi Kalka showed higher antioxidant activity for the DPPH radical scavenging, anti-inflammatory activity, and anti-tyrosinase activity for the assays conducted,...
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Posted by admin on Apr 30, 2018 in |
A new, simple, sensitive, precise, accurate and stability indicating RP-HPLC method has been developed for the simultaneous estimation of salbutamol and beclomethasone in combined tablet dosage form. The analysis was carried out at 230 nm and this chromatographic separation was achieved with Kromasil 250 C18 [250 x 4.6 mm, 5µ] column under 25 oC temperature and using mobile phase water and acetonitrile in a ratio of 55:45 v/v adjusted pH 2 with ortho phosphoric acid. The retention times of salbutamol and beclomethasone were found to be 2.030 ± 0.01 min, 3.271 ± 0.1min respectively. The proposed method was validated according to ICH guidelines. The linearity study of salbutamol and beclomethasone was found in concentration range of 25 – 150 µg/ml and 50 – 300 µg/ml and correlation coefficient (r2) was found to be 0.999 and 0.999. The percentage recovery was obtained as 98.61 % and 100.75 % w/v for beclomethasone and salbutamol. The LOD, LOQ values were obtained from regression equations of beclomethasone and salbutamol were 1.13 µg/ml, 2.06...
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