Posted by admin on Jan 31, 2018 in |
The decrease in susceptibility towards commonly administered antibacterial agents is due to excessive use of antibiotics every now and then, due to which the resistance toward these antibiotics is developed in pathogens. To overcome the problem of resistance in pathogenic microbes, the study of incorporation of Selenium metal to probioticts supplement was carried out. Antimicrobial activity of individual broth obtained from five different lactobacillus spp. viz. Lactobacillus helveticas, Lactobacillus fermentum, Lactobacillus acidophilus, Lactobacillus gasseri strains with varying concentration of Selenium (20mg/mL, 40mg/mL, 60mg/mL and 80mg/mL) were studied against pathogens like Staphyllococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Candida albicans. The results showed that the antimicrobial property of the probiotic broth was significantly increased and showed good bacteriostatic activity against on Staphyllococcus aureus, Escherichia coli and Candida albicans. The report and such kind of further studies may pave the way for further investigations on the use of pure metals as effective antibacterial agents to overcome the problem of resistance in...
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Posted by admin on Jan 31, 2018 in |
Khat is chewed traditionally in Ethiopia, Somalia, Yemen and Kenya. In Ethiopia, khat chewing is now becoming an everyday substance of abuse for the general population. Knowledge about the effects of khat chewing on blood glucose levels is very sparse. Therefore, the objective of this study was to investigate the effect of fresh juice of khat (Catha edulis) on blood glucose levels of normal and streptozocin-induced diabetic rats. Sixteen normal and 24 diabetic male wistar rats were studied. Diabetic rats were divided into three groups: group I were treated with 4.5 ml/kg of fresh juice of Catha edulis (N=8). Group II were treated with glibenclamide (5 mg/kg) (N=8), group III were treated with distilled water which served as controls. Normal rats were divided into two groups: group I were given 4.5 ml/kg of fresh juice of Catha edulis (N=8) and group II were given distilled water as a control. The rats in each group was orally administered with single dose of fresh juice, glibenclamide or distilled water daily and...
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Posted by admin on Jan 31, 2018 in |
The present study was aimed at induction of callus, micropropagation of Justicia wynaadensis (Nees) T. Anderson in vitro by using different explants and study of the phytocomponents present in the aqueous and methanol extracts of callus and leaf of in vitro propagated plants through GC-MS analysis. Callus was induced using MS basal medium supplemented with combinations of 2, 4-D, IAA and NAA growth hormones in various concentrations. For micropropagation hormone free MS basal medium and MS basal medium with BAP in various concentrations was used. Callus were obtained on MS medium supplemented with 2mg/L 2, 4-D. In vitro shoots were initiated on hormone free MS basal medium and well developed multiple shoots were regenerated on MS basal medium containing 1mg/L BAP. Four different extracts were prepared from callus and leaf of in vitro obtained plants and their phytochemical composition were analyzed by GC-MS. The extracts revealed the presence of various phytocomponents, with therapeutically important properties....
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Posted by admin on Jan 31, 2018 in |
Objective: To evaluate in vitro antioxidant and anti-inflammatory activity of Cassia auriculata leaf extracts. Methods: Herbal extraction was done by Soxhlet extraction method with increasing polarity of Solvents viz., Chloroform, Ethanol, Methanol. Phytochemicals analysis was done using different biochemical tests. Antioxidant potential of plant extracts were analyzed by ferric ion reducing antioxidant power, phosphor-molybdenum and 2, 2-diphenyl-1-picrylhydrazyl, and anti inflammatory activity by using protein denaturation in vitro bioassay. Total phenolic content of each extract was also determined to assess their corresponding effect on antioxidant capacity of plant. Results: Phytochemicals analysis showed that each solvent extract contained broad spectrum of secondary metabolites, phenolic compounds, flavonoids, tannins and glycosides, where as compared to other solvent extracts, chloroform extract showed little quantity of phenolic Compounds, methanol extract exhibited the highest phenolic content and the significant Antioxidant capacity based on the test performed. Out of all extracts, methanol extract showed high anti-inflammatory activity. Conclusion: The present study revealed that different solvent extracts of Cassia auriculata leaves contain broad spectrum of bioactive compounds. Results...
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Posted by admin on Dec 31, 2017 in |
Highly substituted thiophene derivatives first synthesized by Gewald synthesis in 1965, are important heterocyclic compounds present in numerous biologically active compounds. The synthesis of title compounds was carried out by preparing derivatives of Gewald product. In the present study, a series of 2-aminothiophene derivatives were first prepared by Gewald reaction and further converted into 2-aminothiophene-1, 2, 4-triazole derivatives. Knoevenagel condensation of aryl ketones (1a-1d) with ethylcyanoacetate and subsequent treatment of the α-β-unsaturated nitriles (2) with sulfur and amine resulted in the corresponding 2-aminothiophenes (3a-3d), which were used as a starting material for the synthesis of substituted Gewald products. In the subsequent steps (3a-3d) were converted into carbohydrazides (4a-4d). Substituted carbohydrazides were then converted into potassium aryl carbonyl hydrazine carbodithionate (5a-5d) which were further converted into final compound 2-amino thiophene-1, 2, 4-triazole derivatives (6a-6d). The structures of all the synthesized compounds were confirmed by their spectral data (IR and 1H NMR...
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