Posted by admin on Mar 31, 2018 in |
The analgesic activity was assessed using hot plate method, formalin induced paw licking test, acetic acid induced writhing test. Preliminary investigations were carried out with water, DMSO and chloroform for solubility. For analgesic activity 1, 2, 3- triazine-4-ones series of compounds CP1, CP2, CP3 showed significant increase in jumping time of mice in hot plate method at only higher dose level (50 mg/kg). In acetic acid induced writhing test in mice at higher dose levels CP1, CP2, CP3, (50 mg/kg) significantly inhibited the number of writhing in mice. 1, 2, 3-triazine-4-ones series of compounds CP1, CP2, CP3 at (25 and 50 mg/kg) significantly inhibited formalin induced paw licking at both early and late phases. These results indicate that compound possessed both central and peripheral analgesic actions. It was found that the CP1, CP2, CP3 compounds shown good analgesic activity in formalin induced paw licking test at both dose levels (25 and 50 mg/kg) but in other two model hot plate method and acetic acid induced writhing model; shown good...
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Posted by admin on Mar 31, 2018 in |
The MAP kinase phosphatases regulate the magnitude and kinetics of MAP kinase signalling within the cell and as a consequence cell cycle progression and division. Therefore, these enzymes have been implicated in the regulation of number of disease conditions. One member of this family, MKP-2, has been linked to cancers of the breast and prostate. However, in human cells correlating changes in MKP-2 expression and effects upon ERK signalling, the main substrate for MKP-2 has been difficult, due to lack of convincing cellular analysis. Therefore, we utilized a novel lentivirus shRNA MKP-2 construct (L.001) to examine the consequences of knockdown on the regulation of ERK MAP kinase signalling. Infection of LNCaP AI cells with lentivirus shRNA MKP-2 reduced endogenous MKP-2 mRNA by approximately 80% in LNCaP cells. Immunofluorescent GFP labelling revealed strong cellular expression of MKP-2, approximately 70% of the cells were infected. MKP-2 knockdown reduced adenoviral induced hMKP-2 expression by approximately 50%. Both EGF and FCS induced strong but transient activation of ERK phosphorylation in LNCaP (AI), EGF...
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Posted by admin on Mar 31, 2018 in |
Medicinal applications of metals and its compound were reported centuries ago but it gained huge appreciation after the discovery of anticancer activity of metal based drug cisplatin. After cisplatin discovery many medicinal synthetic chemist moved into the field of organometallics for combating diseases and investigating the excellent pharmaceutical potential of metal complexes, giving birth to modern medicinal organometallic chemistry. Derivatization of the already existing drugs is the emerging field of pharmaceutical and synthetic medicinal chemistry. Working on metal complexes of already approved drugs can substantially reduce the time and money spent in clinical development. Organotin(IV), Cu(II), Zn(II), Fe(III) and Sb(III) complexes of Ribavirin were synthesized keeping the same in mind. These complexes were characterized by elemental analysis, IR, 1H, and 13C NMR spectroscopic studies. The synthesized complexes and the parent drug were screened for in-vitro biological activities. Interestingly some of the synthesized metal complexes of ribavirin have shown impressive antibacterial and antifungal on the other hand ribavirin was found to be...
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Posted by admin on Mar 20, 2018 in |
The present study was designed to compare the chemical and phytochemical compositions of Momordica charantia, Azadira chtaindica, Trigonella foenum-graecum and Phyllanthus emblica and subsequently, antidiabetic activities of T. foenum-graecum and a combination of polyherbal extract of T. foenum-graecum and M. charantia were evaluated on alloxan induced diabetic mice. The proximate and phytochemical compositions of the plants were quantitatively determined using standard methods. In order to evaluate antidiabetic activity, normal and alloxan induced diabetic mice were orally administered 0.5 mL aqueous extract of T. foenum-graecumand combination of T. foenum-graecum – M. charantia once per day for 7 days. These two plants were selected for antidiabetic activity based on their high phytochemical contents. A near normal blood glucose value of 98.5 ± 0.2 mg / 100mL and 97.02 ± 0.55 mg / 100 mL was observed in the 7th and 5th day experimental animals after administration of T. foenum-graecum and T. foenum-graecum – M. charantia, respectively and remained at the same level even after further oral administration. Consequently, it is evident...
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Posted by admin on Mar 8, 2018 in |
Osteoporosis is defined as low bone density with micro architectural deterioration of bone tissue leading to enhanced bone fragility and increased fracture risk. Knowing the treatment pattern of osteoporosis is imperative in achieving better health outcomes. Thus the purpose of our study was to assess the treatment pattern of osteoporosis in 8 different medical colleges and hospitals in Bangladesh situated in 2 different districts, Dhaka and Comilla between the periods of September 2015 to April 2016. A pre-designed questionnaire was given to 107 doctors in the hospitals and data was collected on sociodemographic and professional characteristics of the participating physicians along with their learning opportunities and different parameters of treatment and diagnosis. Majority of doctors, 89.7% had internet access at work place for getting updated on osteoporosis, 84.1% attended specialized osteoporosis programs but only 63.6% had subscription in medical journal or website, Around 58.9% practitioners used guidelines for osteoporosis and a greater percentage of doctors, 15.1% preferred bisphosphonates for treatment of osteoporosis. Preferred diagnostic tool was dual-energy X-ray absorptiometry...
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