Posted by admin on Nov 30, 2017 in |
Atenolol and esmolol drugs belonging to a β–blocker group are frequently used for the treatment of cardiovascular diseases. These drugs are also charged as water pollutants. A new simple and rapid spectro-photometric method has been developed for their determination in pharmaceutical formulations and in spiked water samples. The proposed method is based on the derivatization of the amino function present in these drugs to the corresponding yellow copper (I) drug dithiocarbamate derivative through reaction with carbon disulphide, pyridine and copper (I) perchlorate in aqueous acetonitrile. The analysis is accomplished for their determination in pharmaceutical formulations by measuring absorbance at 425 nm for atenolol and 420 nm for esmolol. For the determination of these drugs in spiked water samples, the coloured complex is extracted into chloroform and the absorbance of the chloroform extracts is measured at 415 nm and 410 nm for atenolol and esmolol respectively. Linear correlation is obtained in the range 1.00 – 22.00 and 1.50 – 25.00 µg mL-1 with detection limits of 0.10 and 0.15µg mL-1...
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Posted by admin on Nov 30, 2017 in |
The aim of the present study is to investigate and evaluate analgesic and anti-inflammatory potential of aqueous and methanolic extracts of Drosera spatulata using different models in rats. The aqueous and methanolic extracts of D. spatulata (at the doses of 3, 4, 5, 8 and 10 mg/Kg body weight) investigated for anti-inflammatory and analgesic activities using various experimental models. Analgesic activity was evaluated using formalin induced paw licking models in rats. Anti-inflammatory activity was evaluated using measurement of paw edema volume model in rats. Our results showed that both extracts caused a significant (P < 0.05) dose-dependent reduction of inflammation and pains induced by agent used. These results provide support for the use of aqueous and methanolic extracts of Drosera spatulata in relieving inflammatory, pain and insight into the development of new agents for treating inflammatory and pain diseases but further studies are needed to elucidate the mechanism (s) of action and phytochemical constituents of the...
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Posted by admin on Nov 30, 2017 in |
Bioavailability of orally administered cinnarizine is typically low and variable due to high incidence of degradation. Many methods are available in public domain for the determination of Cinnarizine in plasma by RP-LC method but none published a LC-MS/MS method. Our objective is to develop a simple, rapid, sensitive bioanalytical method for the determination of Cinnarizine in human plasma by LC-MS/MS. Method has been developed for the Calibration curve range 0.4 to 100 ng/mL in plasma using LC-ESI-MRM detection on API 4000 coupled with SIL-HTC. Chromatography separation was achieved on Hypurity C18 column using Methanol: 5 mM Ammonium Acetate (pH 4.5) buffer in the ratio 95:5. Sample extraction was performed with methyl tertiary butyl ether and the detection was performed for MRM 369.250/ 167.150 along with labeled internal standard Cinnarizine D8. Method validation was performed for selectivity, matrix effect, recovery, precision, accuracy, ruggedness, haemolysis and lipemic effect and stabilities in aqueous as well as in plasma, all validation parameters found well within acceptance limits. Then the method is successfully applied...
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Posted by admin on Nov 30, 2017 in |
Background: Hypercholesterolemia is rapidly becoming a worldwide epidemic that affects children and adults. Some attempt had made to generalize a relationship between hypercholesterolemia and infertility, but until now it remains scanty. Thus, the aim of the present study was initiated. Methods: Male Wistar rats were divided into four groups of six each (n=6). The first group was control and second, third and fourth groups were fed with pure cholesterol and cholic acid orally once a day for 30 days. Body weights were noted weekly. At the end of treatment duration all animals were sacrificed by cervical dislocation. Epididymis and testis were carefully removed and the organ weights were evaluated. Left epididymis was used for sperm count and sperm morphology. Blood sample were separated and used for determination of MDA level. Serum was used to determine the Total cholesterol (TC), Triglyceride (TG), HDL levels. LDL, VLDL levels were calculated. Results: Cholesterol-fed animals showed significant increases in obesity index and blood TABARS levels. TC and TG and LDL levels were also...
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Posted by admin on Nov 30, 2017 in |
Drug interaction generally refers to a modification of the expected drug response in the patient as a result of exposure of the patient to another drug or substance. Some unintentional drug interactions produce adverse reactions in the patient, whereas some drug interactions may be intentional, to provide an improved therapeutic response or to decrease adverse drug effects. They are divided into three groups depending on the underlying mechanism of interaction: pharmaceutical, pharmacokinetic or pharmacodynamic...
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