Posted by admin on Feb 28, 2018 in |
Seaweeds form economically important component of marine ecosystems worldwide. A wide range of beneficial and detrimental interactions exists between seaweeds and epiphytic as well as endophytic bacteria. Diversity study can be used to retrieve ecological information about community partner that relates the degree of stability of that community. The present work deals with the study the microbial diversity of Ulva lactuca. Epiphytic and endophytic bacteria were isolated from U. lactuca. Total of 4 bacterial isolates were obtained from this seaweed. Among them 2 (VP2 and VP4) were endophytic bacteria and 2 were epiphytic bacteria (VP6 and VP7). Gram staining and biochemical tests for identification of micro-organisms were performed. On the basis of colony morphology and biochemical tests, VP2, VP4 and VP6 were identified as Enterobacter aerogenes, Bacillus cereus and Staphylococcus aureus respectively. Out of 4, 1 isolate could not be identified on the basis of biochemical tests performed. Hence, molecular characterization was attempted by targeting 16S rDNA sequencing for the fourth isolate, VP7. The amplified product was identified as...
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Posted by admin on Feb 28, 2018 in |
The dramatic increase in the burden of chronic non-communicable diseases (NCDs) in emerging economies has become a huge challenge and as such led to increased reliance on folkloric medicine for NCDs management. Consequently, wild Olea europaea leaves were assessed for medicinal potentials, as they are commonly used as a traditional therapy for these diseases in Eastern Cape, South Africa. The antioxidant and antibacterial properties of the O. europaea extracts obtained using different solvents by maceration method were tested by spectrophotometric, disc diffusion and agar dilution methods respectively, while the essential oil obtained in a Clevenger modified apparatus was analyzed by GC-MS. The O. europaea extracts showed moderate to strong inhibitory potential in scavenging the three different tested radicals in a concentration dependant-manner. The ethyl acetate leaf extract (MEA) and ethanol extract (ME) were active against the growth of the six tested bacteria in particular Klebsiella pneumoniae and Serratia marcescens. Gas chromatography – mass spectroscopy studies on the essential oil revealed 61 compounds, accounting for 93.031% of the total oil....
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Posted by admin on Feb 28, 2018 in |
Olmesartan medoxomil (OLM) is a lipophilic (log P = 4.31) antihypertensive drug suffering from limited oral bioavailability in humans (26%) due to its low aqueous solubility, uncontrolled enzymatic conversion to the active metabolite (Olmesartan; OL) and efflux by drug resistance pumps. Surmounting such limitations via incorporation of OLM into self-nanoemulsifying drug delivery systems (SNEDDS). Based on OLM-equilibrium solubility studies in various oils, surfactants and co-surfactants, Capmul® MCM, Tween® 20, Cremophor® EL and polyethylene glycol – 400 (PEG) were combined in different ratios to plot ternary phase diagrams. OLM-loaded SENDDS were developed and evaluated forparticle size, polydispersity index (PDI), zeta potential, self-emulsification time, morphology, drug released percentages after 5-min (Q5min%), 1 hour (Q1h%) and dissolution efficiency percentages (DE1h%). The OL pharmacokinetics from SNEDDS (F6) and Benicar® tablets were evaluated (LC-MS/MS) in rabbits. Spherical OLM-loaded SNEDDS were developed. The best-achieved SNEDDS (F6) showed short emulsification time (13 s), fine droplet size (60.00nm), low PDI (0.25), negative zeta potential (-14.4mV), promising dissolution parameters; Q5min% (29.78%), Q1h% (66.69%) and DE1h% (47.96%) and enhanced...
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Posted by admin on Feb 28, 2018 in |
Tadalafil belongs to BCS class IV and recommended for treatment of erectile dysfunction. Micronization technique is used by researchers to improve its solubilization and dissolution rate. The conventional tabletting excipients pose challenge especially with micronized drug particles. The current research work aimed to study the effect of some speciality excipients on flow properties of micronized Tadalafil formulated wet granulation approach. Different grades of L-HPC and Lactose were studied. L-HPC grades included L-HPC NBD 21, L-HPC 21 and HPC – LM. The lactose grades included Milled lactose (Pharmatose 200M) and Granulated lactose (Supertab GR and Supertab SD 11). Milled lactose was used in formulation using wet granulation process whereas granulated lactose was used as directly compressible excipient. PVP k-30 added intra-granularly in concentration range of 1 – 3% w/w. All the formulation batches were evaluated for its micromeritics and dissolution. The batch with L-HPC NBD 21 showed ‘Excellent’ flow properties as compared to L-HPC LM and L-HPC 21. Both grades of granulated lactose showed ‘Excellent’ flow of granules but slightly...
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Posted by admin on Feb 28, 2018 in |
Objective: Antimicrobials have become one of the most misused therapeutic agents resulting in resistance. Present study was carried out to evaluate the antimicrobial drug utilization pattern. Method: A retrospective, observational record based study was done in hospitalized patients from medicine and surgery department of tertiary care hospital of central India over a period of one year. Result: Among 385 patients, 53.25% were females and 26.75% were in 41 – 50 years age group. Amongst 775 antimicrobials prescribed, 75% were injectables. Average number of antimicrobials per prescription was 2.01 ± 0.6. Use of antimicrobial monotherapy (39.74%) was common and 25.71% had more than two antimicrobials. About 33.16% drugs were prescribed by generic names and FDCs were prescribed in 37.14%. Culture and sensitivity tests were done in 35.6% cases. Cephalosporins (65.19%) were the most preferred antimicrobials followed by penicillins, aminoglycosides and antiamoebics. Ceftriaxone, amikacin and metronidazole were the most commonly prescribed antimicrobials. Prescribed daily dose (PDD) was greater than defined daily dose (DDD) for ceftriaxone, amikacin, amoxicillin, metronidazole and levofloxacin indicating...
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