Posted by admin on Jan 31, 2018 in |
The objective of the present study is to develop and validate a novel stability indicating UV spectrophotometric method for estimation of Benzydamine Hydrochloride (BNZ) in pharmaceutical dosage form as not a single UV spectrophotometric method has been reported for the estimation of Benzydamine Hydrochloride, which makes the present work novel. In the present research work, UV spectrophotometric determination was carried out at an absorption maximum of 305.6 nm in pH 6.8 phosphate buffer. The purposed method was validated statistically for linearity, accuracy, precision, repeatability, ruggedness, sensitivity as per International Conference on Harmonization guidelines. The drug substance was exposed to acid hydrolysis, alkaline hydrolysis, oxidative degradation, photolytic degradation, and thermal degradation and the stressed samples were analyzed by the proposed method to demonstrate the specificity of the method. The application of developed method was established by performing the assay of pharmaceutical formulation. The proposed method obeyed beer lambert’s law in the concentration range of 5 – 50 µg ml-1 with correlation coefficient (r2) of 0.999. Percentage relative standard deviation associated...
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Posted by admin on Jan 31, 2018 in |
Reverse Phase – High performance liquid chromatographic (RP – HPLC) method was described for determination of Pseudoephedrine Sulphate (PSE) and Loratadine Hydrochloride (LOR). The chromatographic separation was achieved using mobile phase mixture of Acetonitrile: 0.05 M Potassium Dihydrogen Phosphate Buffer: (pH 2.8 adjusted with Ortho Phosphoric Acid Solution): Methanol in the ratio of 30:35:35 (%v/v/v) with column Zorbax Eclipse XDB C18, (150 x 4.6 mm i.d), Particle size 5 mm at 1.2 ml/min flow rate. 10 µL of standard preparation containing 150 µg/ml PSE and 6.25 µg/ml LOR was injected into the column and the component was separated by carrying out elution for a run time of 10 minutes and detected at 254 nm wavelength. The described method shows excellent linearity over a range of 120 to 180 μg/ml and 5 to 7.5 μg/ml for PSE and LOR, respectively. PSE and LOR were subjected to stress degradation conditions of hydrolysis (acid and base), oxidation, Heat and UV light degradation. Stressed samples were analysed by the developed method. The proposed...
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Posted by admin on Jan 31, 2018 in |
The decrease in susceptibility towards commonly administered antibacterial agents is due to excessive use of antibiotics every now and then, due to which the resistance toward these antibiotics is developed in pathogens. To overcome the problem of resistance in pathogenic microbes, the study of incorporation of Selenium metal to probioticts supplement was carried out. Antimicrobial activity of individual broth obtained from five different lactobacillus spp. viz. Lactobacillus helveticas, Lactobacillus fermentum, Lactobacillus acidophilus, Lactobacillus gasseri strains with varying concentration of Selenium (20mg/mL, 40mg/mL, 60mg/mL and 80mg/mL) were studied against pathogens like Staphyllococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Candida albicans. The results showed that the antimicrobial property of the probiotic broth was significantly increased and showed good bacteriostatic activity against on Staphyllococcus aureus, Escherichia coli and Candida albicans. The report and such kind of further studies may pave the way for further investigations on the use of pure metals as effective antibacterial agents to overcome the problem of resistance in...
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Posted by admin on Jan 31, 2018 in |
Khat is chewed traditionally in Ethiopia, Somalia, Yemen and Kenya. In Ethiopia, khat chewing is now becoming an everyday substance of abuse for the general population. Knowledge about the effects of khat chewing on blood glucose levels is very sparse. Therefore, the objective of this study was to investigate the effect of fresh juice of khat (Catha edulis) on blood glucose levels of normal and streptozocin-induced diabetic rats. Sixteen normal and 24 diabetic male wistar rats were studied. Diabetic rats were divided into three groups: group I were treated with 4.5 ml/kg of fresh juice of Catha edulis (N=8). Group II were treated with glibenclamide (5 mg/kg) (N=8), group III were treated with distilled water which served as controls. Normal rats were divided into two groups: group I were given 4.5 ml/kg of fresh juice of Catha edulis (N=8) and group II were given distilled water as a control. The rats in each group was orally administered with single dose of fresh juice, glibenclamide or distilled water daily and...
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Posted by admin on Jan 31, 2018 in |
The present study was aimed at induction of callus, micropropagation of Justicia wynaadensis (Nees) T. Anderson in vitro by using different explants and study of the phytocomponents present in the aqueous and methanol extracts of callus and leaf of in vitro propagated plants through GC-MS analysis. Callus was induced using MS basal medium supplemented with combinations of 2, 4-D, IAA and NAA growth hormones in various concentrations. For micropropagation hormone free MS basal medium and MS basal medium with BAP in various concentrations was used. Callus were obtained on MS medium supplemented with 2mg/L 2, 4-D. In vitro shoots were initiated on hormone free MS basal medium and well developed multiple shoots were regenerated on MS basal medium containing 1mg/L BAP. Four different extracts were prepared from callus and leaf of in vitro obtained plants and their phytochemical composition were analyzed by GC-MS. The extracts revealed the presence of various phytocomponents, with therapeutically important properties....
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