Posted by admin on Oct 31, 2017 in |
In the present study Terminalia bellirica seeds, were evaluated for the In vitro anti-oxidant activity and phytochemical evaluation using different solvents such as methanol, ethylacetate, chloroform and aqueous. The in vitro antioxidant activity of Terminalia. bellirica is screened by standard antioxidant assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radicals and Total antioxidant activity by ABTS assay. The ethylacetate extract of T. bellirica seed extract was found to be relatively high activity (84±0.6) than other extracts. Similarly the ethyl acetate exhibits high antioxidant status in ABTS and hydroxyl scavenging assays. In comparison to other extracts the ethyl acetate and methanolic extract found significant and prominent antioxidant activity due to the presence of high phenolic and steroid content. Finally this study concludes that all the seed extracts of T. bellirica exhibiting high antioxidant potential with respect to dose depending...
Read More
Posted by admin on Oct 31, 2017 in |
Here, we evaluated the in vitro antileishmanial activities of Olea europaea linn. leave extract (OLE) on some Leishmania spp. OLE (0–160 ?g/mL) was considered against promastigote and intracellular amastigote forms of L. tropica (MHOM/IR/2002/Mash2) and L. major (MRHO/IR/75/ER) using MTT assay and in a macrophage model, respectively. The obtained findings demonstrated that OLE significantly (?<0.05) inhibited the growth rate of promastigote and amastigotes as a dose and time dependent response. To conclude, the current investigation exhibited effective antileishmanial activity of...
Read More
Posted by admin on Oct 31, 2017 in |
The present study on Embilica officinalis, a common household remedy and the present study describes the inhibition of enzyme xanthine oxidase (XO) with in vitro analyses and assenting in silico study to produce an effective phytoconstituents. The results explained the ethanolic extract of Embilica officinalis (EOEt) exhibited antioxidant and defensive oxidative stress and allied with its total phenol (51.33 ± 0.793 mg/g) and flavonoid content (33.51 ± 0.616 mg/g). Additionally, the DPPH scavenging activity shows significant correlation between antioxidant and XO inhibitory activities result in IC50 value of 40.40 ± 0.1475µg/ml. The EOEt extract probably inhibited the nitric oxide formation with increase in concentration with IC50 of 28.36 ± 0.1522µg/ml. The EOEt extract shows increase in dose dependent manner in percentage of XO inhibitory activity and exhibits potential IC50 value (352.0 ± 0.2069 µg/ml) compared to standard allopurinol (723.9 ± 0.2081µg/ml). The in silico docking studies of the major phytoconstituents (Phyllembilic acid B, Ethyl gallate, Gallic acid, 5-Hydroxymethylfurfural, Ascorbic acid, 1, 2, 3-benzenetriol, Ethyl alpha-d ̶ glucopyranoside and β...
Read More
Posted by admin on Oct 31, 2017 in |
Naproxen, a non-steroidal anti-inflammatory drug suggested for the long term treatment of disease conditions such as Rheumatoid Arthritis and Osteoarthritis poses an immediate risk of stomach ulceration. To avoid this risk, a proton pump inhibitor is often prescribed along with it. In the present study, Naproxen (500mg) is prepared in combination with Pantoprazole, a proton pump inhibitor as a multi-layer coated tablet. First, Naproxen is prepared as core tablet by wet granulation method. Then it is enteric coated to assist its delayed-release. Over this enteric coated tablet, Pantoprazole (20mg) drug layer is applied to get combination product. The prepared tablets were evaluated for physicochemical properties such as weight variation, hardness, friability, disintegration and drug content. The in-vitro drug release studies were conducted for Naproxen core tablets, Naproxen enteric coated tablets and multi-layered tablets in 0.1N HCl and 7.4 pH phosphate buffer. The analytical results obtained at several stages of preparation of the product were found to be satisfactory. The in-vitro drug release studies of Naproxen and Pantoprazole from multilayered...
Read More
Posted by admin on Oct 31, 2017 in |
Stress is one of the most significant health problem in modern society. This paves way for the researchers to focus and investigate into the biological pathways linking stress and health. Hence the goal of the present study is to discover novel drug derivatives from the active principle α asarone of the plant Acorus calamus Linn. (ACL) which was found to have antioxidant &antidepressor effect the basic physiological response to stress is through activation of Corticotrophin –releasing factor Receptor 1 (CRFR-1) which is predominantly found in pituitary gland is mainly responsible for signalling the adrenal gland receptor thereby increasing the Glucocorticoids as a response to stress. The 3D structure of CRFR 1 was derived from RCSB-PDB database and the α asarone was derive from Pubchem database. A total of 100 ligands were derived from α asarone using in silico method by ACD chemsketch software. Rapid virtual screening of these compounds were performed in the dock in tool iGEMDOCK v2.0. Based on the binding energy a total of seven ligands were...
Read More
