Posted by admin on Sep 30, 2017 in |
The objective of this study was to design and develop poly herbal cream based formulation using combination of aqueous poly herbal extracts of Rosa damascena flowers (RDW), Clerodendrum paniculatum leaves (CPW), Murraya koengii leaves (MKW) and Annona squamosa leaves (ASW) which increased the collagen content in human dermal fibroblast thereby showing skin anti-ageing property. The presence of collagen –I content in human dermal fibroblast (HDF) cells was confirmed by ELISA. The three different ratios of plant extracts blends (CB 01, CB02 and CB03) were prepared. The extract blend (CB03)in particular ratio (2:2:1:1) of the above individual plant extracts respectively showed 18.65 ± 0.061ng/ml (p < 0.05) of collagen-I as compared to 16.53 ± 0.064 of standard ascorbic acid (ng/ml) at 100µg/ml. Five different carrier cream base was developed (F-1, F-2, F-3, F-4 and F-5. F2 (PHY/AAC/01) formulation was finalised with (CB03) active blend (PHY/AAC/02) which showed 17.53 ± 0.011ng/ml for collagen-I content in HDF cells. The in vitro safety of (CB 03) and (PHY/AAC/02) was measured by neutral red...
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Posted by admin on Sep 30, 2017 in |
The mycological analysis of stored seed of groundnut (Arachis hypogaea) through agar plate as well as blotter paper methods of study revealed presence of 16 fungal species viz., Alternaria alternata, Aspergillus candidus, Aspergillus flavus, A. Niger, A. ochraceous, A. phoenicis, A. tamari, A. terreus, A. sydowi, Fusarium moniliforme, F. oxysporum F. solani, P. glabrum, Rhizopus nigricans, Trichoderma viride and Trichothecium roseum. Out of 20 essential oils extracted from leaf of plants and assessed for their antifungal potential only leaf oil of Tinospora cordifolia,(Willd.) Miers ex Hook. F. & Thoms exhibited absolute toxicity (100%) against dominant fungi Aspergillus flavus, A. niger at 400 ppm concentration. This showed fungicidal activity at 500ppm.It showed MIC against 10 fungi at 400ppm, controlled 15 fungi at 600 ppm. There was no ill effect of physical factors viz., temperature treatment up to 100 °C, autoclaving at 15 lb/square inch pressure at 120 °C and storage up to 105 days on activity of leaf oil. In vivo studies depict that oil of T.cordifolia as seed dressing...
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Posted by admin on Sep 30, 2017 in |
The present study instantiates the phyto-fabrication of silver nanoparticles by utilizing Petrea volubilis L. leaf broth which were instantaneously synthesised, eco-friendly, and cost-effective. The influence of physico-chemical parameters viz. contact time, leaf broth quantity, pH, temperature, and silver nitrate concentration were investigated and optimised to engineer infinitesimal nanoparticles. The SPR bands obtained from UV-vis spectroscopy confirmed the synthesis of silver nanoparticles. The active phytochemicals in Petrea volubilis L. leaf broth namely carboxylic acids, phenolic compounds and aldehydes were found to be responsible for the bio reduction and capping of silver ions according to FT-IR spectroscopy. The capping molecules of NPs were negatively charged and were passably stable as revealed by zeta potential measurements and the XRD patterns illustrated their crystallinity. HRTEM microphotographs enumerate spherical shaped nanoparticles. The EDS analysis at 2θ confirms the synthesis of elemental silver. The nanoparticles were evaluated for their antimicrobial activity. The highest anti-bacterial activity was observed against S. aureus as compared to the bacteria S. typhi and A. flavus a fungus. The leaf extract...
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Posted by admin on Sep 30, 2017 in |
Herbal medicines are effective in the treatment of various ailments. Very often these drugs are unscientifically exploited and / or improperly used. Therefore plant drugs deserve detailed studies in the light of modern science. The detailed investigation and documentation of plants used in local health traditions and pharmacological evaluation of these plants and their taxonomical relatives can lead to the development of invaluable plant drugs for many diseases. The present study has made an attempt to demonstrate the hepatoprotective activity of aqueous and alcoholic root extracts containing and in protecting the CCl4 induced hepatocellular damage. The aqueous root extract of Aegle marmelos showed the presence of carbohydrates, phenols, flavonoids, glycosides, saponins and terpens, proteins and steroids. Significant amount of steroids were present. The alcoholic root extract of Aegle marmelos showed the presence of alkaloids, carbohydrates, phenols, flavonoids, glycosides, saponins and terpens, proteins and steroids. The aqueous and alcoholic extract of given root extracts at two dose levels 200 mg/kg and 400 mg/kg body weight administered orally for five days...
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Posted by admin on Sep 30, 2017 in |
Objective: This study aimed to investigate the in vitro effect of the extracts of five Iranian medicinal herbs on Histomonas meleagridis protozoan parasite. Methods: This study was conducted in the following three phases in Khorramabad, Iran, in 2016: 1) preparing and extracting the 5 medicinal herbs Dracocephalum kotschyi, Satureja khuzestanica, Pulicaria gnaphalodes, Olea europaea, and Echinophora platyloba DC; 2) culturing the positive cases of fecal samples in the fortified commercial medium RPMI1640 up to 1×106 parasite/ml of the parasite; and 3) investigating the effect of 1.25, 2.5, and 5 mg/ml densities of each extract and metronidazole compared with the negative control on hours 2, 4, 6, 24, and 72 with Trypan blue vital stain. Results: In this study, the D. kotschyi extract which destroyed 80% of the parasites in the first two hours was the most, and the S. khuzestanica extract was the second effective extract. However, metronidazole destroyed 60% of the parasites in the first two hours and 100% in the third day and may be excreted before...
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