Posted by admin on Sep 30, 2017 in |
This investigation was aimed to study the effect of few selected antioxidants, Vitamins and minerals for prevention of gastric erosion in indomethacin induced gastric mucosal damage in rats. Male Wistar rats, weighing between 200 -250 g were divided into 9 groups of 6 animals each (n = 6). The groups were treated respectively as follows Group I normal control and Group II disease control received normal saline, Group III was treated with standard drug Omeprazole, Group IV to IX received test substances for 7 days. Ulceration was induced by a single oral administration of indomethacin (30 mg/kg). Various parameters like, the volume, pH of gastric juice, total acidity, ulcer index, percentage protection, mucin content, pepsin activity and antioxidant enzymes were estimated. Histopathology of stomach epithelium was observed. The ulcer index and total acidity were significantly reduced (p < 0.05). Increase in pH was observed in ulcer induced rats pre-treated with test substances. Mucin content was found to be restored significantly in all rats pre-treated with test substances, and pepsin...
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Posted by admin on Sep 30, 2017 in |
Obesity refers to abnormal or excessive fat accumulation in the human body which results in health risk. The aim and objective of the present study is to perform in-silico docking analysis of the phytoconstituents identified in two medicinal plants namely Cinnamomum verum (cinnamaldehyde, eugenol, cinnamyl acetate, caryophyllene and cinnamic acid) and Coriandrum sativum (nerolidol, geranyl acetate, decanol and linalool) on HMG CoA reductase an eznynme target for antihyperlipidemic activity. The phytoconstituents of the medicinal plants were retrieved from pubchem chemical database. The target for docking study is selected as 3-hydroxy-3-methylglutaryl-coenzyme A (HMG CoA reductase). The 3D protein structure of the enzyme HMG CoA Reductase is obtained from Protein Data Bank [PDB ID: 3CCT]. In-silico docking analysis was performed by using Molegro virtual docker (MVD). The parameter used for docking analysis are MolDock score, Rerank score and hydrogen bond interactions. The docking score and the binding pattern of the phytoconstituents are compared against the standard drugs. The MolDock score of standard drugs atorvastatin, pitavastatin and simvastatin was found to be...
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Posted by admin on Sep 30, 2017 in |
A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of nitrofurantoin in the Human Plasma. The Mass transition of nitrofurantoin and losartan (Internal standard) were M/z 237.1/151.9 and M/Z 421.300/ 179 in ESI Negative ionization. Linearity was observed between the nitrofurantoin concentration and the peak area ratio from 10.451 to 1033.897 ng/mL with R2 value of 0.99. Plasma samples containing nitrofurantoin were extracted with Acetonitrile: 5mM Ammonium Acetate (pH 3.8) (80:20). The observed recovery of nitrofurantoin was 90%. The intra-day and inter-day accuracy was performed. Stability parameter was performed. The method will be used in the determination of the pharmacokinetic parameters of nitrofurantoin....
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Posted by admin on Sep 30, 2017 in |
Lead induced oxidative stress implies morphological dysfunctions and physiological deformation in the liver. The aim of present study was to establish the protective effect of chloroform extract of Murraya koenigii leaves against the lethal effects of lead on the antioxidant status of mice. Murraya koenigii Chloroform Extract (MKCE) was prepared by maceration. Male Swiss albino mice were divided into three groups of six each. Group I (Control group) served as normal diet and water ad libitum, Group II (lead treated group) received an intraperitoneal injection of lead acetate (15mg/kg) daily once whereas Group III (MKCE with lead-treated group) received MKCE (50 mg/kg) orally along with a single of injection of lead acetate intraperitoneally (15mg/kg) daily once. Experimental study was continued for the consecutive seven days. On the 8th day, liver and kidneys were desiccated; homogenized in normal saline to collect supernatant into vials for estimating serum malondialdehyde, antioxidant enzymes like superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase, glutathione-s-transferase and ferric reducing ability of plasma levels. It was observed that...
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Posted by admin on Sep 30, 2017 in |
Algae are extensively used in cosmetics, supplements and pharmaceutical industries. Researches support their hypotensive, cytotoxic, anti-inflammatory and many other biologic effects. In the present study, the cytotoxic, antioxidant and acetylcholinesterase inhibitory activities of two brown algae, Padina tetrastromatica and P. australis, have been evaluated. Total extract was prepared using methanol 80%. The extracts were evaluated for antioxidant and acetylcholinesterase inhibitory activity and cytotoxic properties against A-549, HT-29, HepG-2 and MCF-7 cell lines through DPPH, Ellman and MTT assays, respectively. The results revealed that P. australis with the IC50 value of 163.9μg/mL demonstrated stronger antioxidant activity compared to P. tetrastromatica (IC50 259.1μg/mL) in DPPH assay. Neither of the total extracts showed cytotoxic activity up to the maximum amount examined (100μg/mL). The total extracts of P. australis and P. tetrastromatica in concentration of 300μg/mL presented 15.6% and 23.7% enzyme inhibition in Ellman assay, respectively. It was concluded that the two algae have shown weak antioxidant and acetylcholinesterase inhibitory activity and their further use in prevention and treatment of diseases needs more...
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