Posted by admin on Sep 30, 2017 in |
Nanotechnology is an innovative and escalating field that has made its contribution to all spheres of human life. Conventional synthesis of nanoparticles suffers from serious problems like high temperature, poor yield, longer reaction time, and use of toxic and highly sensitive compounds. Green synthesis of nanoparticles is an emerging field which is known to eliminate / minimize the aforementioned drawbacks. In the present investigation, stable silver nanoparticles were synthesized in good yield by chemical reduction of silver nitrate in ionic liquids such as [BMIM] BF4 (IL-1), [BMIM] PF6 (IL-2), [BEMIM] BF4 (IL-3) and [BEMIM] PF6 (IL-4) at room temperature. X-ray diffraction (XRD) and scanning electron microscopy (SEM) have been employed for the characterization of structure and morphology of the synthesized silver nanoparticles. The as synthesized nanoparticles and pure ionic liquids were screened for their anti-microbial activities and it was found that both the pure ILs and silver nanoparticles possess significant antimicrobial...
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Posted by admin on Sep 30, 2017 in |
The aim of the present study was to formulate topical microsponge-based delivery system containing ketoconazole for controlled release of the drug for proficient treatment of fungal infections. Ketoconazole loaded microsponges were prepared by quasi emulsion solvent diffusion method using ethyl cellulose N22 polymer with varied drug polymer ratios. The prepared microsponges were characterized by SEM, FTIR, and evaluated for surface morphology, % drug loading, particle size, % entrapment efficiency and in vitro drug release. The effect of formulation variables such as drug to polymer ratio, stirring speed on the physical characteristics of microsponges was examined. Compatibility studies using UV and FTIR indicated that there is no chemical interaction between drug and polymers. SEM studies revealed that the prepared micro sponges were spherical and porous with a mean particle size of 82.25μm. The formulations were subjected to in vitro release studies for 8 hr which showed sustained release. Microsponges were further incorporated in to carbopol gel formulation for topical delivery. Prepared gel formulations were evaluated for physical parameters like pH,...
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Posted by admin on Sep 30, 2017 in |
Association of memory impairment (MI) with epilepsy, majorly in temporal lobe epilepsy (TLE) is considerably a tricky condition where existing treatment options involving the administration of anti-epileptic drugs (AED’s) tend to worsen the stated incidence. This study evaluates the aggravating effect of valproate (VAL) at normal and reduced doses on MI associated with TLE and additionally assesses the protective effect of levetiracetam (LEV) on this undesirable link. Albino mice of either sex (n = 36) were employed for the study period of 64 days where seizures were induced by intraperitoneal administration of pilocarpine followed by evaluation of anti-epileptic activity by technique of Racine scores. Assessment of errors, a factor for degree of MI was executed by employment of radial arm maze. Finally brain biochemical measures of Acetyl cholinesterase and glutamate along with cresyl violet staining and estimation of total neuronal number of the hippocampus were performed. Both at normal and reduced doses of VAL exhibited augmentation of MI on temporal lobe epileptic mice. Although reduction in VAL dose brought...
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Posted by admin on Sep 30, 2017 in |
Clerodendrum phlomidis is a shrub common in India and Sri Lanka. It is one of the highly traded medicinal plants from tropical forests, as the leaves and roots are used in folk-lore, Ayurveda, Siddha and Unnani medicines. In this study preliminary phytochemical screening, cytotoxic, antioxidant and GC – MS study of leaf of C. phlomidis was evaluated. Successive extraction of the leaf of C. phlomidis was carried out with 5 solvents petroleum ether, chloroform, ethyl acetate, methanol and water. Antioxidant activity was evaluated using DPPH free radical scavenging assay at four different doses of 31.25, 125, 500 and 1000 µg/mL, cytotoxic potential was studied by brine shrimp lethality assay at four different doses of 1, 10, 100 and 1000 µg/mL. GC-MS analysis of methanol leaf extract was carried out on Thermo GC-TRACE ultra version: 5.0, Thermo MS DSQ II. Phytochemical screening analysis of the extracts indicated the presence of secondary metabolites such as steroids, steroidal glycosides, phenols, saponins, coumarins, alkaloids and flavanoids. Methanol leaf extract showed significant cytotoxic potential...
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Posted by admin on Sep 30, 2017 in |
Medicinal properties in Lawsonia inermis are due to the presence of bioactive compounds and can also show adverse effects at high concentrations. In the present study we have determined the nontoxic concentration of hexane, ethyl acetate and methanol fractions of plant leaves. 50 wistar albino rats of both sexes were used as experimental animal and were randomly divided into 4 groups viz. Group 1 (control), Group 2 (Hexane fraction), Group 3 (Ethyl acetate Fraction) and Group 4 (Methanol Fraction). Groups 2, 3 and 4 were further divided into following sub groups, Group 2A (H) (100mg/kg), Group 2B (H) (250mg/kg), Group 2C (H) (500mg/kg), Group 3A (H) (100mg/kg), Group 3B (H) (250mg/kg), Group 3C (H) (500mg/kg), Group 4A (H) (100mg/kg), Group 4B (H) (250mg/kg) and Group 4C (H) (500mg/kg). Rats in Group 1 (control) received 3% DMSO as solvent for dissolving fractions. All the doses were administered orally with the help of polythene cannula and were given once in a day, followed up to 21 days. After 21 days, blood...
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