Posted by admin on Oct 31, 2017 in |
A thermostable bacterial 3-cyanopyridinase enzyme has been identified that catalyze 3-cyanopyridine to nicotinic acid and ammonia. Nicotinic acid is mainly used in food, pharmaceutical and biochemical industries. This study was undertaken to optimize the purification of 3-cyanopyridinase enzyme and characterization of its various kinetic properties. The partial purification of enzyme has been carried out in five steps that results in approximately 2.3 fold increased purification with 46.5 % of enzyme recovery. The partially purified enzyme was observed approximately 43 kDa of maximum activity at 50 °C temperature and pH 7.0. The partially purified enzyme showed the Km of 0.91 mM and maximum velocity (Vmax) of 0.59 U. The half-life (t1/2) of partially purified enzyme was about 60 min at optimal conditions. The enzyme has broad substrate specificity, hydrolyzing toxic compounds such as benzonitrile and propionitrile. Thus this novel enzyme has potential in bioremediation process....
Read More
Posted by admin on Oct 31, 2017 in |
To investigate the content of polar auxin transport inhibitor flavonoid quercetin (Q) and topo-isomerase I-DNA inhibitor alkaloid camptothecin (CPT) from Gracilaria corticata (GC), red seaweed from Surathkal beach, Karnataka a comparative study was carried out. Methods: The content of Q and CPT was quantified in the ethanolic extract of GC using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The HPLC analysis resulted in a sharp peak of authentic CPT and Q at the retention time of 2.445 min and 3.540 min respectively. The peaks of ethanolic extract of GC spiked with CPT was found at the retention time of 1.630 min, 1.690 min and 2.000 min and retention time in the ethanolic extract of GC spiked with Q was found to be 1.600 min and 1.713 min. The method developed for this study shall stand appropriate for the quantification of secondary metabolites and screening of flavonoid and alkaloid contents in the...
Read More
Posted by admin on Oct 31, 2017 in |
In present study antiproliferative activity of Rutin was evaluated on HeLa cell line induced cervical cancer in rats. For this study, 30 rats were divided into 5 groups and each group containing 6 rats each. Group I- normal saline treatment for 45 days, Group II- cancer cells (1×106 cells in 0.1ml/rat), Group III– 5-Fluorouracil (20mg/kg + 1×106 cells in 0.1ml/rat), Group IV- Rutin (50mg/kg + 1×106 cells in 0.1ml/rat), Group V- Rutin (70mg/kg + 1×106 cells in 0.1ml/rat). After 24 h of tumour inoculation intraperitoneally, Rutin was administered daily for 45 days. After administration of last dose followed by 18 hrs fasting, rats were sacrificed for observation of antiproliferative activity. The change in body weight, body circumference of tumour bearing hosts and simultaneous alterations in haematological profile, serum (Triglycerides, Total protein, Total cholesterol, GGT, ALP and glucose) and liver biochemical parameters (lipid peroxidation, GSH and antioxidant enzymes-CAT, GPx) were estimated. The changes in tissue enzymes-Glucose-6 phosphate dehydrogenase, Hexokinase, Succinate dehydrogenase and CytochromeP450 levels were also estimated. Rutin maintained the...
Read More
Posted by admin on Oct 31, 2017 in |
Olmesartan medoxomil, a BCS class II drug, is an angiotensin-II receptor antagonist used for the treatment of hypertension. A liquisolid tablet of Olmesartan medoxomil was developed to improve its dissolution and flow properties. The commonly used carrier Avicel was compared with Neusilin. Because of higher liquid load factor, Neusilin was selected as a carrier for further optimization study using 32 Factorial design. Two independent factors, % drug concentration in a liquid vehicle (X1) and carrier coating ratio (X2) were studied, each with three levels and the systems was assessed for two dependent variables; % drug dissolution and angle of repose. Mathematical equations and response surface plots were used to relate the dependent and independent variables. All prepared liquisolid formulations were characterized for pre-and post- compression parameters. The optimized formulation F7 (15% drug concentration and 30 carrier coating ratio) was tested for X-ray powder diffractometry and FTIR. Results showed that maximum drug dissolution was exhibited by systems with minimum drug concentration in liquid vehicle and optimum carrier to coating ratio....
Read More
Posted by admin on Oct 31, 2017 in |
The present study was initiated to quantify the Isoflavones content that accumulated in soybean transgenics with RnPPARγ gene by using high performance liquid chromatography. The effects of methanolic leaves extract of transformed soybean in 3T3-L1 adipocyte cell line. The RnPPARγ transgenic soybean variety JS335 was developed and transgenics were confirmed by PCR and Southern blot techniques. The quantification of isoflavones compounds by HPLC showed that methanolic leaves extracts of transformed soybean contained Genistein (1.67µg/g extract), Daidzein (2.76µg/g extract), Rutin (32.06µg/g extract) and Quercetin (48.40µg/g extract) and methanolic leaves extracts of non-transformed soybean contained Genistein (1.23µg/g extract), Daidzein (2.33µg/g extract), Rutin (26.16µg/g extract) and Quercetin (43.03µg/g extract) by comparing the chromatogram of the reference standards. In the present study revealed that secondary metabolite content was higher in the transformed plant than non-transformed plants. After treatment of transformed methanolic leaves, extracts on 3T3-L1 mature adipocytes significantly ameliorated lipid droplet accumulation and droplet reduction in adipocytes might be the result of lipid metabolism by...
Read More
