Posted by admin on Jul 31, 2017 in |
This study reports the phytochemistry of the oil obtained from the air-dried non-utilized fruits of H. madagascariensis a plant widely used in folklore medicine. The H. madagascariensis fruits oil (HMO) was obtained by de-fatting the chloroform extract of the air-dried fruits with n-hexane. The n-hexane extract further separated on a normal phase silica column to afford the HMO. The acute toxicity test was done on mice using the Lorke’s method. Selected physico-chemical properties (specific gravity, refractive index, pH, viscosity and saponification value) were evaluated using standard procedures. Characterization of the fatty acids constituents was done using Gas Chromatography-Mass (GC-MS) and Fourier Transform Infra Red (FTIR) spectroscopic techniques. The HMO (reddish oil) had LD50 > 5000 mg/kg; specific gravity = 0.918; refractive index = 1.472; pH: 6.4, Viscosity: 2.252, Saponification value (mg KOH/g): 140.5. GC-MS characterization of the HMO afforded eight fatty acids derivatives (47.93% unsaturated fatty acid and 14.82% saturated fatty acids of the total peak area): palmitic acid methyl ester (7.00%), palmitic acid (2.61%), linoleyl alcohol (1.22%), linoleic...
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Posted by admin on Jul 31, 2017 in |
Naproxen and Ibuprofen, two non-steroidal anti-inflammatory drugs, are frequently prescribed in modern medicine. In this study, nanoencapsulation by solvent evaporation technique is used to formulate Naproxen and Ibuprofen loaded poly(DL-lactide-co-ε-caprolactone) nanospheres by means of Tween 80 and Span 80 as surfactant and co-surfactant respectively. Different formulations are prepared with different drug masses and same quantities of polymer and surfactants. This was done in order to evaluate the effect of the mass variation of the two drugs on the characteristics of the prepared nanospheres. The prepared nanospheres are studied for drug entrapment and drug loading efficiencies, particle size, morphology, FT-IR analysis and in vitro release. As a result, all nanospheres presented a spherical profile and homogeneous size distribution. Naproxen revealed the highest drug entrapment efficiency. FT-IR analysis showed no interaction for both drugs with the polymer. In vitro release of drugs from encapsulated PLC nanospheres showed that Ibuprofen release was faster than Naproxen release. Also the in vitro release was faster for formulations having the lowest amount of drug...
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Posted by admin on Jul 31, 2017 in |
In the present competitive world, stress is a major cause for several health disorders– Hypertension, Cardiovascular disorders, Supressed Immunity and other conditions. Proper stress management takes on great importance given the wide range of bodily systems impacted by stress hormones. Cortisol – the “stress hormone” affects the liver by increasing several biochemical pathways. One of the prominent stress marker is increase in gluconeogenesis. Due to the growing importance of herbal medicines, Clerodendrum colebrookianum was chosen for the study, commonly called as Phuihnam by the Mizo tribe of north eastern India. Previously carried out qualitative studies indicated the presence of alkaloids, flavonoids, steroids, saponins, phenolscardiac-glycosides and anthraquinones in Phuihnam. Ex-vivo studies were carried out using the hepatocytes from Sus scrofa domesticus. Our study indicated a high glucose release having an absorbance about 0.335 ± 0.001 at 520 nm after one hour of incubation when cortisol was induced but on addition of the boiled extract the absorbance drastically reduced to 0.124 ± 0.010 at 520 nm. Similarly on addition of sodium...
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Posted by admin on Jul 31, 2017 in |
Peptides are recognized for being highly selective efficacious and at the same time, relatively safe and well tolerated. Consequently, there is an increased interest in peptides, in pharmaceutical research and development (R&D) and approximately 140 peptide therapeutics are currently being evaluated in clinical trials. Ultrasonic is an area of intense scientific and technological research, science and technology of ultrasonics are widely used in the recent years for industrial and medical applications. Ultrasonic investigation in non- aqueous solutions of electrolytes and non- electrolytes with amino acids provide useful information in understanding the behaviour of liquid/solution system. In the present work, the ultrasonic velocity of a peptide with drug in formamide have been measured at various concentrations and at different temperatures. Using the measured value the thermodynamic parameters and acoustical parameters are calculated. These parameters have been utilized to study the interionic and intermolecular interactions existing in the system. The results arrived from ultrasonic methods have been corroborated with antimicrobial...
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Posted by admin on Jul 31, 2017 in |
In the present study, the hypolipidemic activity of Piper longum (Family: Piperaceae) fruit extract was studied in two experimental models of hyperlipidemia. In an acute model of hyperlipidemia, induced by injecting a single dose of triton WR-1339 (400 mg/kg, b.w.) intraperitonially in rats, administration of Piper longum extract at the dose of 500 mg/ kg, b.w. resulted in significant lipid lowering effect of total cholesterol (TC), phospholipids (PL), triglyceride (TG) and reactivation of post heparin lipolytic activity (PHLA). We observed significant decrease in TC, PL and TG levels by 32%, 19%, 39%, respectively, and increase in PHLA activity by 29% as compared to hyperlipidemic animals. In the chronic model, hyperlipidemia induced by cholesterol rich-high fat diet, we recorded significant decrease in TC, PL and TG levels by 27%, 15%, 34%, respectively, and increase in PHLA activity by 20 % as compared to hyperlipidemic animals. The treatment with fruit extract of Piper longum (500 mg/ kg b.w) simultaneously for 15 days also caused lowering of lipid levels in liver homogenate,...
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