Posted by admin on Aug 31, 2017 in |
Bacterial pathogens are known to pester Mankind from times immemorial; even though significant efforts in getting rid of these harmful microbes have been made the results are very faint with only few organisms that have been eradicated, such as Small pox virus. But the efforts to eradicate bacterial diseases have shown no passable results as in case of viruses. This is due to the exceptional adaptation and transformation abilities of bacteria to varying environmental Conditions. Though a large number of antibiotics are being used from decades now, there are no affirmative solutions available due to resistance developed towards antibiotics by these bacteria. This resistance developed by the bacteria calls for dire necessity to discover new drugs which can at least reduce the hazards posed by these microbes if not eradication. Hence in this study we have focused on bio prospecting of Clerodendrum paniculatum and Saraca asoka against the highly virulent and extremely adaptable organisms E. coli and K. pneumoniae which currently pose a severe threat to humans due to...
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Posted by admin on Aug 31, 2017 in |
A series of novel 2-Benzamido-3-(4-nitrophenyl)-N’-(substituted) benzylidene-acrylohydrazide (4a-l) analogues were designed by incorporating the few pharmacophoric fragments to enhance the activity profile of molecules. Title compounds were synthesized by the microwave irradiation of α-Benzamido-(4-nitro)-cinnamahydrazide (3) reveals with aromatic/ heteroaromatic aldehydes under acidic conditions, characterized by IR, 1H & 13C NMR, Mass and evaluated for antimicrobial activity by using Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Candida albicans strains. Compounds 4e and 4k exhibited significant protection against bacterial strains (MIC: 3.12-50µg/ml; 3.12-100µg/ml respectively) and compound 4h showed better activity (MIC: 12.5 µg/ml) against fungal strain. And also molecular docking interactions with FAB protein for evaluating the antimicrobial activity was done by using XP GLIDE module of Schrodinger suite and this study highlights, all the analogues exhibited significant affinity towards the 5BNM (FAB Protein) have good docking scores (-4.51 to -8.78) than standard drug ciprofloxacin (-4.74). Of the 12 new analogues, compound 4e was identified as most active ligand with good activity profile against bacterial strains and also have higher...
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Posted by admin on Aug 31, 2017 in |
Aim and Objective: To evaluate the antioxidant property of fish oil by its free radical scavenging activity using three different antioxidant models. Materials and Methods: In vitro antioxidant activity of fish oil was evaluated by DPPH radical scavenging activity, lipid peroxidation, ABTS radical scavenging activity using olive oil as positive control. Different concentrations of the fish oil, 0.02 – 4 mg/ml were used for the free radical scavenging activity by different method and the IC50 value was calculated. Results: Different concentrations of the fish liver oil exerted a dose dependant free radical scavenging activity on the different antioxidant models used in the study. The maximum inhibition was 96.87±0.76% at 4mg/ml in case of DPPH free radical scavenging activity with IC50 value 0.148mg/ml. The maximum inhibition on LPO activity was 96.10±0.38% at 4mg/ml with IC50 value of 0.265mg/ml. The fish oil also exerted its maximum inhibition 90.21 2.10% on ABTS free radical scavenging activity at 4mg/ml with IC50 value of 0.331...
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Posted by admin on Aug 31, 2017 in |
The prescription pattern of analgesic was investigated for the months of June, July, August 2016 by studying 253 (143 male + 110 female) BHTs of in patients of the orthopaedic department. The data mainly age, sex, diagnosis and line of management available from BHTs was analysed in the form of percentage, average of variable. The generic name cost of treatment per patient and rationality of prescription were evaluated by referring NLEM, CIMS, WHO indicators of drug utilisation. A total of 599 analgesics were prescribed to the patients. The average number of analgesic per prescription was 2.33. The maximum number of patients was in the age group of 41-51 years. Diclofenac was the most commonly prescribed analgesic 152 (26.2%).Only 51 patients received single analgesic and majority of patient received parenteral (45.6% i.m) preparations. Only 9 (1.0%) analgesics from NLEM but no generic name drug was prescribed. And again gastro protective drugs 240 (95.17%) and antibiotics 290 (114%) were frequently prescribed with the analgesics. The average cost of treatment per patient...
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Posted by admin on Aug 31, 2017 in |
The aim of this research was to formulate and evaluate microspheres of Fluoxetine Hydrochloride. It is a selective inhibitor of serotonin reuptake type of drug used in the treatment of depression. It is practically soluble in water belongs to BCS class I with a bioavailability approximately 72%. Fluoxetine HCl mucoadhesive microspheres were prepared by using chitosan polymer and emulsion solvent evaporation method which enhance bioavailability of drug. Microsphere with particle size in the range 192.92µm to 251.18µm was prepared. Fluoxetine HCl microspheres were evaluated for mean particle size, the percentage yield, entrapment efficiencies, in-vitro release, in vitro mucoadhesive, FTIR, DSC, X-ray diffraction studies and stability study. Formulation F9 microspheres batch was found to be optimized and followed zero-order release kinetic. The optimized formulation was mucoadhesive in nature. Stability studies were carried out for F9 at a temperature of 40±2 °C/RH 75±5% formulation revealed that the drug behaviour was within permissible...
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