Posted by admin on Jun 30, 2017 in |
A sensitive method was developed to determine Ethinyl Estradiol in human plasma by liquid chromatography-tandem mass spectrometric (LC–MS/MS). Separation of analyte and the Internal Standard (IS) Ethinyl Estradiol-d4 using SPE followed by LLE in plasma with TBME, was performed on a LC/MS/MS-API-5500 instrument with SB C18 HT (50mm×3.0mm) 1.8 µm columns at a flow rate of 0.300 ml/min with mobile phase consisting of Acetonitrile: 2 mM Ammonium Formate Buffer (80:20 v/v). The interface used with the API 5500 LC/MS/MS was used a turbo ion spray. The positive ions were measured in MRM mode for the analyte and IS. The method was linear over range of 5.000 – 308.560 pg/mL. The correlation coefficient is ≥ 0.9942 for Ethinyl Estradiol. The mean % recovery of extraction across QC of Ethinyl Estradiol in plasma was 68.48%. The reproducibility of Ethinyl Estradiol QC sample at room temperature and at 5 ºC was between 91.80 to 101.20% and 90.63 to 101.44% respectively. The % CV of intra and inter day precision were less than...
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Posted by admin on Jun 30, 2017 in |
Zanthoxylum armatum is administered as a hepatoprotective agent during indigestion and hepatic related diseases by traditional healers. So, the aim of this study was to investigate the hepatoprotective activity of ethanol extract of Z. armatum (ZAE) in Paracetamol induced hepatotoxicity in albino rats in comparison with the activities of the known and potent hepatoprotective standard drug Silymarin. Treatment with ZAE showed a significant (p< 0.001 – 0.05) decrease in SGOT, SGPT, direct bilirubin and total bilirubin when compared to paracetamol induced control group while the serum level of protein particularly albumin is elevated with the treatment of ZAE in comparison to normal rats and Silymarin treated paracetamol induced group. This means that treatment with ZAE significantly reversed the hepatic changes produced by paracetamol treatment in rats. The possible mechanism of action may be explored by studying the antioxidant effect of the ZAE. The present study demonstrates that the ethanol extract of Z. armatum significantly protects the liver against paracetamol induced hepatic toxicity in healthy albino...
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Posted by admin on Jun 30, 2017 in |
Background: A novel reconstitutable suspension of Paracetamol loaded microspheres was prepared using ethyl cellulose as a release controlling polymer and by solvent evaporation technique for the relief of osteo-arthritic pain. Objective: The formulation was developed to enhance patient compliance of population suffering from dysphagia or have difficulty in swallowing oral solid dosage forms like tablets and capsules. Method: Microspheres were evaluated for percent yield, drug loading, and entrapment efficiency, in vitro drug release profile and particle size. The microspheres were formulated as a dry oral suspension which has to be reconstituted at the time of use. Result: The particle size of microspheres was in the range of 550 -700 μm. The entrapment efficiency was in the range of 82.95- 95.56% and the yield obtained was 91.13-98.76%. The dissolution studies of selected trial showed a percent cumulative drug release of not less than 85% at the end of 3 hours. Formulation trial P5containing 4% xanthan gum, 5% purified talc and 6 mg sucralose gave a suspension having good flow property,...
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Posted by admin on Jun 30, 2017 in |
Our previous study highlighted the antimicrobial activity evaluation of Nigella sativa seed oil extract that was obtained from supercritical fluid extraction using carbon dioxide (SCFE-CO2) on Gram positive pathogenic bacteria. The aim of this study was to formulate oil-in-water (o/w) emulsion of previous N. sativa seed oil extract and to investigate its antimicrobial activity against Streptococcus mutans and Streptococcus sanguis as the most common causative bacteria of dental caries. Oil-in-water emulsion containing 10% N. sativa seed oil extract (DLBS1355) exhibited good inhibition activity on both S. mutans and S. sanguis. Time-kill assay and biofilm inhibition assay were conducted using emulsion with 0.5% N. sativa seed oil extract content. The results showed bactericidal and biofilm inhibition activities on both tested bacteria. Antimicrobial activity of the emulsion was found higher on S. sanguis than S. mutans. Results of the present study showed that emulsification process did not significantly affect the antimicrobial activity of N. sativa seed oil extract and the formulated N. sativa oil-in-water emulsion could further be used as an...
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Posted by admin on Jun 30, 2017 in |
Plants have always been traditionally used to address various disorders. Due to their rich phytochemical profile, they have been employed in a gamut of skin care and concomitant treatment of disorders. In this Study, we screened the aqueous (CAE), hydroalcoholic (CHE) and ethanolic (CEE) extract of leaves of Cinnamomum verum for its activities The antioxidant potential of the extracts were determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Potential) assays. The extracts showed good activity with increasing concentration. Further, extracts were screened for anti-Inflammatory property by HRBC membrane stabilization assay and Inhibition of Protein Denaturation. The results of anti-inflammatory activity were compared with standard control values. CHE was found to exhibit better activities as compared to the other two extracts and was subsequently screened for Tyrosinase inhibition, Elastase inhibition and Hyaluronidase inhibition activity. CHE showed moderate anti-inflammatory and Hyaluronidase-inhibition activity. CHE can therefore be used as a potential bioactive component in skin care...
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