Posted by admin on Jun 30, 2017 in |
Our previous study highlighted the antimicrobial activity evaluation of Nigella sativa seed oil extract that was obtained from supercritical fluid extraction using carbon dioxide (SCFE-CO2) on Gram positive pathogenic bacteria. The aim of this study was to formulate oil-in-water (o/w) emulsion of previous N. sativa seed oil extract and to investigate its antimicrobial activity against Streptococcus mutans and Streptococcus sanguis as the most common causative bacteria of dental caries. Oil-in-water emulsion containing 10% N. sativa seed oil extract (DLBS1355) exhibited good inhibition activity on both S. mutans and S. sanguis. Time-kill assay and biofilm inhibition assay were conducted using emulsion with 0.5% N. sativa seed oil extract content. The results showed bactericidal and biofilm inhibition activities on both tested bacteria. Antimicrobial activity of the emulsion was found higher on S. sanguis than S. mutans. Results of the present study showed that emulsification process did not significantly affect the antimicrobial activity of N. sativa seed oil extract and the formulated N. sativa oil-in-water emulsion could further be used as an...
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Posted by admin on Jun 30, 2017 in |
Plants have always been traditionally used to address various disorders. Due to their rich phytochemical profile, they have been employed in a gamut of skin care and concomitant treatment of disorders. In this Study, we screened the aqueous (CAE), hydroalcoholic (CHE) and ethanolic (CEE) extract of leaves of Cinnamomum verum for its activities The antioxidant potential of the extracts were determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Potential) assays. The extracts showed good activity with increasing concentration. Further, extracts were screened for anti-Inflammatory property by HRBC membrane stabilization assay and Inhibition of Protein Denaturation. The results of anti-inflammatory activity were compared with standard control values. CHE was found to exhibit better activities as compared to the other two extracts and was subsequently screened for Tyrosinase inhibition, Elastase inhibition and Hyaluronidase inhibition activity. CHE showed moderate anti-inflammatory and Hyaluronidase-inhibition activity. CHE can therefore be used as a potential bioactive component in skin care...
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Posted by admin on Jun 30, 2017 in |
Sterility is the complete absence of any viable microorganism in a drug product. The Specification of sterility is unchanging and is independent of the kind of manufacturing Process. Sterilisation of the final product in its container closure system or aseptic manufacturing is followed. Under Terminal sterilisation usually it involves filling and sealing product containers under high quality Environmental conditions. Products are filled and sealed in this type of environment, so as to minimise the microbiological content of the in-process product and also to help ensure that the Subsequent sterilisation process is successful. The main complicating factors are the temperature and humidity of the cleanroom and the variations between people. The length of time will also depend upon the grade of the cleanroom. Critical Consumables are designed to provide protection for the head, body, hands and feet. In the process of establishing a system for garment selection, it is important to consider the broader aspects of cleanroom use. The main purpose of this study is to check the level of...
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Posted by admin on Jun 30, 2017 in |
Dorema ammoniacum gum (DAG) has been used for different illnesses in Iranian traditional medicine. The main objective of the present study was to evaluate the analgesic and anti-inflammatory activities of aqueous solution of Dorema ammoniacum gum (DAG) in animal model. The anti-inflammatory and analgesic Effects of DAG were examined using carraginane-induced edema test, formalin test and acetic acid-induced writhing response. 42 male albino mice randomly assigned in different groups were pretreated randomly assigned 5 groups. Groups were pretreated either with normal saline, morphine or aspirin and different doses of DAG (125, 250, 500 mg/kg, i.p.) for determination of analgesic effect. The anti-inflammatory effect was evaluated by the rat paw carrageenan induced edema and was compared to positive controls. DAG exhibited the analgesic effect in chronic phase of formalin test in a dose-dependent manner (P<0.05). Also, application of different doses of DAG had significant analgesic effect in acetic acid writing test 30 min after its injection. DAG showed anti-inflammatory effect in a dose dependent manner. The anti-inflammatory effect of the...
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Posted by admin on Jun 30, 2017 in |
The roots of Landolphia heudelotti are employed in traditional medicine for the treatment of a plethora of ailments. In this study, the antioxidant activity and bioactive constituents of the methanol root extract were investigated. Fourteen known compounds including a lignan, neolignans, sesquilignans, a coumarin and an aromadendrane sesquiterpene were isolated from the root extract. The structure elucidation of compounds was performed based on mass spectral and NMR spectroscopic data and by comparison with literature. The crude extract had a high total antioxidant Capacity of 108.8 ± 14.52 mg/g of dried extract (ascorbic acid equivalent) and also demonstrated significant DPPH free radical scavenging activity (IC50 = 6.956 ± 0.8121 µg/mL). The extract also had a total phenolic content of 98.14 ± 14.70 mg/g of dried extract (tannic acid equivalent). The results of this study have given scientific credence to the use of L. heudelotti roots in traditional medicine. This is the first report of these phyto-constituents from L....
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