Posted by admin on Jun 30, 2017 in |
A simple, sensitive and stability indicating reverse phase high performance liquid chromatography method (RP-HPLC) was developed and validated for the estimation of Lapatinib and its related substances in bulk and finished dosage forms. The newly developed method was assessed using Zorbax Eclipse C18 (3.5 µm, 100 × 4.6 mm) HPLC column with pH- 4.5 ammonium formate buffer and acetonitrile as mobile phase in gradient elution mode with a run time of 35 minutes. UV detection was carried out at a wavelength of 261 nm with an injection volume of 5 µL. The developed method was validated as per the ICH guidelines with respect to precision, accuracy, linearity, robustness, specificity and system suitability. Lapatinib samples were exposed to thermal, photolytic, acidic, basic and oxidative stress conditions. The stressed samples were analyzed by the developed method to establish the stability indicating power of the method. The LOD values were 0.009, 0.012 and 0.011 µg/ml and the LOQ values were 0.027, 0.035 and 0.0304 µg/ml respectively for impurity-1, impurity-2 and impurity-3 of...
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Posted by admin on Jun 30, 2017 in |
Hypertension is one of the major public health challenges worldwide. Amlodipine, a calcium channel blockers (CCBs), is among the first line antihypertensive drugs used commonly. Amlodipine reduces blood pressure by its vasodilatory action and its adverse drug reactions (ADRs) such as ankle edema, headache and flushing are related with its vasodilatory action. This study is conducted to find the incidence of ankle edema in patients of mild to moderate hypertension treated with Amlodipine 10 mg. A total of 64 informed consented patients, fulfilling inclusion and exclusion criteria were involved in the study. Follow-up visits were performed after four weeks and eight weeks. At each visit, complete clinical examination was carried out, including a recording of systolic and diastolic blood pressure (BP). Safety was assessed in terms of both subjective and objective systemic adverse-effects. In the present study, significant reduction of mean systolic blood pressure (SBP) and mean diastolic blood pressure (DBP) was seen. Commonly seen adverse drug reactions (ADRs) were ankle edema, constipation, headache and fatigue. The association of...
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Posted by admin on Jun 30, 2017 in |
Enoxaparin is useful in the treatment of unstable angina, but its use is associated with many hematological side effects principally bruising appearance. A prospective comparative study was designed to evaluate the effects of certain predictors on the occurrence of bruising side effect of enoxaparin during the administration of two different subcutaneous (SC) doses (prophylactic vs. therapeutic) of enoxaparin injection among patients with unstable angina. Patients were divided into two groups, 60 patients served as A group given a prophylactic dose 40mg q12h. The other 60 patients served as the B group given 1mg/kg q12h. Area of the bruising was measured by disposable measuring tape as mm2. A significant higher occurrence in the mean of bruising area was found in males (P=0.014), geriatrics (P=0.001), patients with body weight more than 76 kg (P=0.007), therapy of 5-day duration (P=0.01), hypertensive patients and those treated with captopril (P=0.001) while receiving the therapeutic dose (B group) than those receiving the prophylactic dose (A group). In conclusion, certain predictors significantly affect the occurrence of...
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Posted by admin on Jun 30, 2017 in |
The objective of present work is to study the wound healing effect of topical lawsone gel. Topical lawsone gel was prepared by using natural and synthetic polymers in different ratios. The gel was prepared with Tara gum and HPMC K15M using dispersion method. Optimized gels were characterized for physicochemical evaluations. Optimised formulations (LT3, LT4) were evaluated for spreadability (10.6±0.02, 13.8±0.28), pH (6±0.58, 5±0.59), viscosity (18000±62.5, 18023±71.3) and drug content (94.51±0.63, 87.08±0.40). The gel was found to be homogeneous and uniform without any formation of lumps. In vitro release of optimized gel (LT3, LT4) showed control release of 81.68% and 87.47% within 7 hours. The formulation was found to follow zero order model dependent kinetics with fickian diffusion possessing a release rate of 198.3 and 202.4 µg /cm2/hr-1/2. Animal studies performed with optimized formulations (LT3, LT4) showed wound contraction of (85.30±1.56%, 99.21±1.32%) when compared to the control gel (76.61±2.15%). The period of epithelisation of LT3, LT4 formulation showed complete epithelisation on 13.4±0.45 and 14.2±0.61 days respectively, when compared to control...
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Posted by admin on Jun 30, 2017 in |
The present study was designed to evaluate the protective effect of omega-3 against β-cyfluthrin (β-cyf)-induced neurotransmitters’ impairment, oxidative damage, testicular toxicity and genotoxicity. Adult male Wistar rats were divided equally into four groups: Group (I): Control, Group (II): β-cyf (5 mg/kg body weight/day), Group (III): omega-3 (400 mg/kg body weight/da y) + β-cyf (5 mg/kg body weight/day), Group (IV): omega-3 (400 mg/kg body weight/day). β-cyf’s treatment for 8 weeks inhibited the activities of AChE, DA, LDH, total ATPase and Na+/K+ATPase. Also, β-cyf resulted in an increase in oxidative stress as indicated by elevations in the levels of MDA and NO while, the levels of TAC, thiol content and TP were decreased in brain and testis. Sperm count, sperm motility and testosterone levels were reduced while, sperm abnormalities, FSH and LH levels were increased in β-cyf-treated group. Also, β-cyf administration led to a significant increase in TNFα, IL-6 and APP mRNA expressions. In addition, β-cyf caused histopathological alterations in brain and testis. The present results showed that omega-3 could improve...
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