Posted by admin on Mar 31, 2017 in |
The present study was designed to evaluate the effect of Helix aspersa crude extract on colonic injury resulting from hyperhomocysteinemia induced by feeding rats high methionine diet. Wistar albino rats (200-250g) were divided into four groups. Hyperhomocysteinemia (Hhcy) was induced by methionine treatment (1g/kg/day) for 15 days. M group received only Methionine, MH and MP received Methionine and Helix aspersa Crude Extract (8g/kg/day) or Prednisolone (4 mg/kg/day) respectively at the 8th day until the end of experimentation. Prednisolone was used as a standard drug. At the end plasma homocysteine and C-reactive protein were evaluated, followed by colon histopathological investigations. Methionine treatment induced significant increase (P <0.001) in homocysteine and CRP levels in plasma as compared to control rats. Helix aspersa crude extract induces significant decreased of homocysteine (P <0.001) and CRP (P <0.01) levels as compared with experimental control group. The results were comparable to those obtained with prednisolone, a standard anti-inflammatory drug. Histological assessment showed colonic tissue damages in methionine treated group compared to control (P <0.001) as...
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Posted by admin on Mar 31, 2017 in |
In recent years, the significance of polyunsaturated fatty acids analysis has gained much attention because of their various biological activities in health and disease, especially the n-3 and n-6 fatty acids. The current proportional cram endeavors on providing qualitatively the fatty acid composition of methanol extracted seed oils of five Artocarpus species specifically Artocarpus heterophyllus, Artocarpus integrifolia, Artocarpus inscicus, Artocarpus hirsitus and Artocarpus integer. All the extracted oils were thick yellowish in colour having pungent odour with a yield of 40%. Fallouts evidently indicate that the intricate fatty acid profile was experiential for A. heterophyllus through fifteen peaks, next to that A.hirsitus by nine peaks, A.integrifolia by seven peaks and, A.inscicus seed oil had merely five peaks lastly A. integer with four peaks. Essential fatty acid 9-Octadecadienoic acid (C18H34O2) and 9, 12 – Octadecadienoic acid, methyl ester (C19H34O2) were found in all tested methanol seed oil free fatty acid methyl ester fractions except A. inscicus in that order. These findings demonstrated that essential oils of five Artocarpus species seeds...
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Posted by admin on Mar 31, 2017 in |
A major part of the total population in developing countries still uses traditional folk medicine obtained from plant resources. Chemical substances used for prevention of dental caries are known to have many side-effects. Thus, natural products should be explored for their anticaries action. Present study evaluate the antimicrobial effect of alcoholic and aqueous extracts of Magnifera indica leaves against oral dental pathogens Streptococcus mutans, Staphylococcus aureus. Ethanolic extract was found to be more effective and dose dependent against both pathogens. Phytochemical and antioxidative study was also performed using various methods of testing on the aqueous and ethanolic extracts for the presence of phenols, alkaloids, saponins, terpenoids, flavonoids, tannins and reducing sugar. Both leaf extracts showed higher anti oxidative capacity and phytochemicals content like alkaloids and steroids which are active antimicrobial components in plants. Present investigation provides preliminary information for using mango leaf extracts in the prevention of oral diseases such as dental...
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Posted by admin on Mar 31, 2017 in |
A novel method of estimation and validation of Atorvastatin by Reverse Phase-High Performance Liquid Chromatography coupled with Ultra-violet detection was developed which had high potential in determining drug concentration in rat plasma samples during preclinical studies. Atorvastatin being >99% protein bound, exhibits great challenge in getting extracted from plasma samples. Hence, by treating them with strong protein precipitating agents, that is, initially with 10% w/v perchloric acid and further treating its supernatant with mixture of 2M potassium carbonate and 3M of potassium hydroxide, drug extraction was facilitated. Diclofanac sodium was the selected internal standard. Process of elution was conducted using Phenomenex C18 column and mobile phase comprising of a mixture of methanol: water in 70:30 ratio adjusted to pH 5.5. This precise method was linear between a range of 10 to 1000 ng/ml with limit of detection and quantification as 10ng/ml and 15ng/ml respectively. 2-factor 3-level face centred Central Composite Design was employed using Design Expert Software ver. 8.0.0 to examine effect of independent chromatographic factors like pH of...
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Posted by admin on Mar 31, 2017 in |
Delivery of the desired drug as bioadhesive drug delivery systems has been subject of interest since 1980s. The various advantages associated with these systems made the buccal drug delivery as a novel route of drug administration. Buccal region offers an attractive route for the administration of systemic drug delivery. The objective of the study was to develop bucoadhesive buccal tablets of Chlorpheniramine Maleate and Phenylephrine Hydrochloride in combined dosage form. Buccal tablets were prepared by Wet granulation method using the Carbopol 934P, Hydroxy propyl methyl cellulose (HPMC), and sodium CMC as bioadhesive polymer. The tablets were evaluated for the precompression parameters and post compression parameter like bioadhesive strength, In vitro retention time, and In vitro drug release study and microbial analysis. The thickness and weight of the tablets, respectively, ranges from 3.82 ± 0.01 and 3.92 ± 0.02 and the weight of tablets ranges from 201-202mg.The Formulation containing sodium CMC and HPMC shows acceptable bioadhesive strength but erode respectively, with in 6 to 8 hours. The tablet formulation containing...
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