Posted by admin on Mar 31, 2017 in |
The early identification of infection from sterile inflammation is still challenging step in nuclear medicine. Therefore, several radiopharmaceutical agents have been examined to find the solution of this dilemma in clinical practice. Recently 99mTc-Ofloxacin has been developed as an infection-seeking radiotracer. This investigation was launched to examine the sensitivity and selectivity of new developed radiotracer to distinct septic from aseptic lesions. The freeze-dried new developed kits were reconstituted by the 740 MBq (20 mCi) freshly 99mTcO4. The radiochemical purity of 99mTc-Ofloxacin was undertaken by thin layer chromatography (TLC) and Radio high performance liquid chromatography (Radio-HPLC) analysis. Twenty adult, male NMRI rats were randomly divide into two groups equally. Infection was induced by S. aureus and sterile inflammation created by Carrageenan test. Then radioisotope studies have been performed after intravenous administration of radiotracer into the contra lateral tail vein in all studies. TLC and Radio-HPLC investigations indicated that the infection-seeking radiotracer could be prepared with appropriate yield. All lesions were visualized by scintigraphy imaging. The quality of images was good...
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Posted by admin on Mar 31, 2017 in |
Probiotics have been used to protect hosts from pathogens of gastrointestinal and reproductive systems, but their ability to protect against systemic pathogens is largely unexplored. In this study we ask whether orally administered bacteria and yeasts can protect Drosophila melanogaster against the opportunistic fungal pathogen Aspergillus flavus. Flies were fed an artificial diet mixed with live microorganisms for one day prior to infection with A. flavus, and mortality was recorded every day for 8 days. Seven microorganisms were tested; of these, Bacillus cereus (ATCC 13061), Candida inconspicua, Issatchenkia hanoiensis, and Klebsiella sp. significantly decreased mortality of flies subsequently infected with A. flavus compared to controls infected with A. flavus alone. Heat-killed microorganisms did not protect flies, suggesting that the probiotic effect observed was not caused by improved nutrition. D. melanogaster is a good model organism to study microbial interactions with hosts and test the effects of potential probiotics against...
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Posted by admin on Mar 31, 2017 in |
To develop pulsatile microspheres of amlodipine and nifedipine drug release, a blend of polymer and solvent were used with pulsatile principles of drug delivery system to modified drug release pattern on and to release the drug at a particular time on the basis of lag time. In this study, we assessed the in vitro drug release pattern of the formulation prepared from different polymers ratio with the drugs of calcium channel blockers i.e. Amlodipine and Nifedipine. Different formulations of Amlodipine and Nifedipine were prepared using polymers Eudragit RS100, Eudragit S100, Carbopol 971P and polyvinyl alcohol. Optimum formulations were selected from both the drugs of pulsatile microsphere formulation such as formulation N12 from Nifedipine drug and A5 from amlodipine drug by conducting evaluation parameters for microsphere. In vitro studies were carried out for both the formulation and compared to each others. Kinetics drug released kinetics model fitted for both the drugs of optimum formulation for N12 and A5. Formulation N12 showed first order kinetics model and formulation A5 showed zero...
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Posted by admin on Mar 31, 2017 in |
Background: Woman’s risk for developing heart disease rises after menopause may possibly correlated with the striking increase in cholesterol levels. Objective: A randomized controlled cluster group single blind study was conducted by treating with dried curry leaf powder and cucumber slices in hyperlipidemic post menopausal women in rural area. Methods: Menopausal healthy women of rural communities (n =40; 45–65 years of age) with hyperlipidemia who were on with or without hypolipidemic drug were treated with dried curry leaf powder (5 g) adding in main side dish during lunch. Similarly another forty who were on with or without hypolipidemic drug were given100-125g cucumber slices before lunch for 45 consecutive days. A group of forty women with or without hypolipidemic drug was kept as the control. The demographic variables were collected using questionnaire. Fasting blood samples were collected before and after the intervention and lipid profile. Results: Among the total 120 subjects recruited, most of them (46.66%) had total cholesterol (TC) 200-239 mg/dl and 26.66% had high cholesterol. The low density...
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Posted by admin on Mar 31, 2017 in |
Objective: To rationalize the macroscopial, anatomical and physico-chemical studies on leaves of plant Dalbergia sissoo Linn. (Fabaceae). Methods: The crude ethanolic extract of leaves of Dalbergia sissoo Linn. (Fabaceae) was using physico-chemical parameters, fluorescence analysis, and preliminary phytochemical investigation (TLC, HPTLC, column chromatography). Results: An attempt has been made to highlight this folk herbal medicine through present study which will assist in the identification of fresh as well as dried crude samples of leaves anatomically and physiochemically. TLC fingerprint profiling and fluorescence analysis of powdered leaves were also carried out and the salient qualitative and quantitative parameters are reported. Conclusions: The present study will provide referential information for correct identification and help in checking adulteration in market samples used in the preparation of various herbal medicines. The present observation will also be helpful in macroscopical, microscopical and physiochemical studies on leaves of Dalbergia sissoo...
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