Posted by admin on Jan 31, 2017 in |
Paper presents a novel high performance liquid chromatography method development, optimization, validation and application of determining blonanserin in bulk drug, its formulation, human serum and in the presence of its degradation products. Selection of important factors which may influence separation were concluded: percentage of acetonitrile in the mobile phase, the percentage of orthophosphoric acid in aqueous phase and flow rate. Factors selected were altered in accordance to Box-Behnken design plan and investigation in factors having significant influence on separation were executed. Finally, the choicest values from solution offered of investigated factors were 43 % of acetonitrile in the mobile phase, 0.03% orthophosphoric acid in water and flowrate of 0.75 ml/min. The method validation was performed according to International Conference on Harmonisation guideline acceptance criteria for robustness, linearity, selectivity, precision and accuracy. Quantitative and qualitative system responses have not significantly affected by little variations in chromatographic parameters, which demonstrates robustness of the method. Limits of quantification and detection for blonanserin was calculated. Method shows excellent linearity, which was confirmed by...
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Posted by admin on Jan 31, 2017 in |
The present investigation was under taken to evaluate the anti-asthmatic activity of ethanolic extract of Citrullus colocynthis Fruits in experimental animals. Citrullus colocynthis Fruits was evaluated for anti-histaminic activity using isolated goat tracheal chain preparation and histamine induced Bronchoconstriction in Guinea pig. Citrullus colocynthis Fruits significantly inhibited dose dependent contraction of goat tracheal chain produced by histamine and also showed significant protection by prolonging Preconvulsion dyspnea time (PCD) in guinea pigs. Thus, Citrullus colocynthis Fruits showed anti-allergic activity against histamine and hence possesses potential role in the treatment of...
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Posted by admin on Jan 31, 2017 in |
Gas Chromatography-Mass Spectrometry (GC-MS) studies play an important role in isolation and identification of phytocompounds. Since, the chemical composition of the plants usually determines its biological activity, phytochemicals of possible pharmaceutical importance can be subjected to a detailed analysis. The present study is concerned with the GC-MS analysis of chloroform extract of the bark of Terminalia travancorensis, an evergreen tree, endemic to the Western Ghats. Reports on the benefits and or medicinal properties of T. travancorensis are rare or even absent. In this context, the present study was carried out to determine the chemical constituents of the bark by GC-MS analysis. Twenty two components were identified by GC-MS analysis in the chloroform extract. The major components detected were Friedelan-3-one (15.93%), gamma-sitosterol (10.48%), alpha -octadecene (8.54%), n-Tetracosanol-1 (8.21%), Phenol, 2,4-bis(1,1-dimethylethyl) (7.77%), 1-Nonadecene (7.49%), Heptacosanol (6.16%), 1-Heptadecene (5.25%) and stigmast-4-en-3-one (5.46 %). Friedelan-3-one, the major compound is a triterpenoid possessing antifeedant, anti-inflammatory, anticancer, hepatoprotective, antimicrobial and anticandidal activities. The second major compound, gamma-sitosterol plays a vital role in the structure and...
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Posted by admin on Jan 31, 2017 in |
Objective: This study aims to formulate novel dorzolamide hydrochloride and timolol maleate ocuserts to enhance patient compliance through providing controlled drugs release from polymeric matrix. Methods: Ocuserts were prepared by solvent-casting method using different polymers Ethyl Cellulose, Eudragit S100 and Hydroxy propyl methyl cellulose in different ratios. The prepared ocusters were physcochemichally evaluated for their weight, thickness, drug content uniformity, surface pH, Swelling Index (SI) and folding endurance. In-vitro drug release was studied from the prepared formulas and the results were analyzed by drug release kinetic models. The ocuserts stability after three month’s storage at 40 ±0.5oC and 75 ±5% RH was estimated. In-vivo tests were done to study the release profile and estimate the safety of the incorporated drugs in rabbits’ eyes. Results: The prepared ocuserts show uniform weight, thickness and drug content. Their surface pH was in the physiological range and showed acceptable folding endurance. HPMC Formulas had higher SI values. Results of in-vivo testing for one of the prepared ocuserts shows slow release of both drugs...
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Posted by admin on Jan 31, 2017 in |
Eriosema psoraleoides leaves have been reported to be used in traditional African medicine to treat cuts and wounds, hence the present investigation of its antibacterial activity using the agar pour plate method. The leaves contain tannins and flavonoids. A methanol extract was obtained from the leaves by maceration, and then the n-hexane and ethyl acetate fractions were obtained from this. Statistical analysis of data was by ANOVA, with p<0.05 considered significant. The methanol extract, n-hexane fraction and ethyl acetate fraction were found active against Staphylococcus aureus ATCC 25923, and Staphylococcus aureus Clinical isolate; in addition, the methanol extract was active against Pseudomonas aeruginosa. However, Staphylococcus aureus Clinical isolate was found resistant to Gentamicin. The Minimum Inhibitory Concentrations (MICs) of the extract and fractions against Staphylococcus aureus ATCC and against Staphylococcus aureus Clinical isolate, showed that ethyl acetate fraction was more active than the other two fractions. Although Staphylococcus aureus was found to be resistant to Gentamicin, it was susceptible to the antibacterial activity of the methanol extract, n-hexane fraction,...
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