Posted by admin on May 31, 2017 in |
Brassica campestris Linn. (yellow mustard), a common Indian condiment, possesses melatonin as an important antioxidant. Optimization of supercritical carbon dioxide extraction (SC-CO2) process parameters was conducted to obtain an extract having the best combination of yield of melatonin and maximum in vitro hypoglycaemic and hypocholesterolemic activities, along with a desirable balance of ω6 to ω3 fatty acids. A full factorial design and response surface methodology were employed for process optimization. Melatonin content of the extracts was obtained by high performance liquid chromatography and by liquid chromatography-mass spectrometry. The optimized extraction conditions were obtained at 300 bar, 50 °C for 120 min. This extract exhibited highest content of melatonin (660.72±41.05 ng/g of dry mustard powder), maximum in vitro hypocholesterolemic (60.11±3.89%) and hypoglycaemic (59.70±2.67µg/ml) activities and possessed minimum ω6 to ω3 (1.29) ratio with minimum erucic acid content (2.00±0.20 g/100g of fatty acid). The electron paramagnetic resonance spectroscopy of the extract (0.10±0.001 g/ml) showed 72.10±4.01% scavenging activity of DPPH radicals, establishing its strong antioxidant potency. A new Chrastil equation was developed...
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Posted by admin on May 31, 2017 in |
The extraction of poly-β-hydroxybutyrate (PHB) and C-phycocyanin (C-PC) from Nostoc muscorum revealed that PHB yield after C-PC extraction was not found differ significantly as without C-PC extraction. Under photoautotrophic growth condition, the test cyanobacterium accumulates PHB and C-PC with a maximum yield of 40 and 57 mg l-1 respectively at the stationary phase, i.e. on day 21 of incubation. The most significant enhancement in C-PC yield up to 146 mg l-1 was recorded in 0.4 % fructose supplementation. High C-PC and PHB yield was observed in 0.4% fructose supplementation followed by 0.4% glucose supplementation. Here, in this study we show, that PHB yield after C-PC extraction was not affected significantly and C-PC yield was found enhanced under 0.4 % carbon source (fructose and glucose) supplementation. Significant Biomass and PHB yield depicted under supplementation of glucose, fructose, maltose and sucrose was due to boost in growth. Thus, present study demonstrates the extraction of both the products, i.e. PHB and C-PC is...
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Posted by admin on May 31, 2017 in |
Aims and Objectives: The present study was conducted to find out morphometry of thyroid gland in different age groups. Material and Methods: The study was conducted on 60 human thyroid glands in the Department of Anatomy, Pt. B. D. Sharma, PGIMS, Rohtak in North Indian population. Results: In present study, mean length of right and left lobe of thyroid gland was found to be 29.88±7.03 mm and 29.45±5.44 mm in Group A, 41.33±6.95 mm and 38.87±5.04 mm in Group B, 35.02±3.98 mm and 31.77±3.27 mm in Group C respectively. The mean width was observed as 18.76±4.23 mm and 20.77±6.01 mm in Group A, 24.13±4.77 mm and 22.47±2.72 mm in Group B, 21.54±2.05 mm and 20.72±2.53 mm in Group C respectively in right and left lobes. The mean thickness was 13.70±4.69 mm and 13.60±4.51 mm in Group A, 15.19±1.99 mm and 15.01±2.29 mm in Group B, 14.73±1.99 mm and 14.09±2.33 mm in Group C respectively in right and left lobes. The mean length and height of the isthmus of thyroid...
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Posted by admin on May 31, 2017 in |
Mental disorders are commonly occurring and often seriously impairing in many countries throughout the world. The total number of people suffering from anxiety or depression amount to 615 million, Close to 10% of the world’s population is affected. Chrysanthemum indicum is an ancient herbal medicine used as an anti-arthritic, anti-inflammatory and anti-anxiety in many Asian countries. The elevated plus-maze (EPM) and open field test has been most familiar tool in the investigation of the neurochemical transmission basis of anxiety, for testing anxiolytic drugs. The anxiolytic activities of Ethanolic and Chloroform extracts of Chrysanthemum indicum Linn flowers were evaluated using the elevated plus-maze (EPM) and Open field. The ethanolic extract results are suggestive of anxiolytic activity in dosages around 250 and 500 mg/kg, as expressed by elevation of the time spent on the open arms in the plus-maze; a decrease of freezing and increase of deambulation in the open field...
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Posted by admin on May 31, 2017 in |
The aim of the present studies is to evaluate the cytotoxic effects of newly formulated polymeric nanoparticles (ETP NP’s) of Escitalopram oxalate (ETP) on NB41A3 cell lines. They were made by ionic gelation method with particle size range between 60 – 115nm, showing spherical morphology. Moreover, zeta potential of the same was recorded as -1.89 ± 0.052 mV and its permeability release showed 98.45 ± 1.82 % in 24 hour. The cytotoxicity evaluation of the optimized formulation (A3) included qualitative method of cell morphology (microscopic and DAPI staining evaluation) and quantitative colorimetric assays like – neutral red (NRU), MTT and LDH. The results suggested that, in presence of ETP, there was a remarkable decrease in cell count whereas; ETP NP’s showed lesser cytotoxicity. performed. Further, in NRU membrane integrity was assessed and it showed 95.2 ± 0.83 % and 72.2 ± 0.56% of cell viability in ETP NP’s and ETP pure respectively. However, MTT assay summed up the cell viability up to 93.6 ± 0.68 % in ETP NP’s...
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