Posted by admin on Feb 28, 2017 in |
Introduction: Wrightia arborea (Apocynaceae) has been used in traditional medicine to treat ailments such as menstrual and renal complaints, snake-bites, tooth-ache and diarrhea. Objective: This study aimed to evaluate the phytochemical constituents, antioxidant, antimicrobial and antiproliferative activities of W. arborea leaf extracts. Materials and Methods: Total phenolic and flavonoid contents of the leaf extracts were determined. The antioxidant activity of the extracts were investigated using DPPH, ABTS, nitric oxide scavenging assays, phosphomolybdenum assay, reducing power and metal chelating assay. Antimicrobial activity was tested against MTCC strains and MDR clinical bacteria. The antiproliferative activity was determined using MTT assay against three cancer cell lines. Results: Extracts showed high total phenolic content ranging from 85.7± 1.15 to 40.3± 0.6 gallic acid equivalents and flavonoid content ranging from 750±5.5 to 140±3.6 quercetin equivalents per gram of dry extract. Ethyl acetate extract showed highest DPPH scavenging activity with an EC50 value of 330μg/ml. The antimicrobial effects of all the extracts were found to be genus specific – only against Klebsiella spp. The chloroform...
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Posted by admin on Feb 28, 2017 in |
Aim: The study was done to evaluate the antibacterial and antifungal activity of the leaves of Colocasia esculenta Linn. Materials and Methods: Fresh tender leaves of C.esculenta collected, air-dried at room temperature, grounded to a fine powder and extracted with ethanol. The antibacterial and antifungal activity of C.esculenta compared to standard antibiotics was assessed by disc diffusion method by measuring the zone of inhibition. The organisms used for the test were Staphylococcus aureus, Klebsiella, Escherichia coli and Pseudomonas aeruginosa and Candida albicans. The agar disc diffusion method described by Kirby-Bauer was used for testing the antibacterial and antifungal activity. Results: Zone of inhibitions produced by sensitive organisms were demarcated by a circular area of clearing around plant extract impregnated discs and measured in millimeter (mm). Mean ± SEM was calculated and the data was statistically analysed by one way ANOVA followed by Dunnets multiple comparison test. Conclusion: In our study, ethanolic extract of Colocasia esculenta showed antibacterial activity against S. aureus, P. aeruginosa, E.coli, Kleibsiella and antifungal activity against...
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Posted by admin on Feb 28, 2017 in |
The studies on tree biomass for assessing carbon density in tropical forest ecosystem of central India was undertaken during 2009-11 in forest area of eight districts [Jabalpur, Seoni, Balaghat, Narsinghpur, Mandla, Dindori, Shahdol and Umaria] at 30 adopted representative sites at varying latitudes and longitudes. The annual rainfall of their study area was 1000-1600 mm. The soils of the study sites were alluvial, light to medium black as well as red and yellow with pH value ranging from 5.5 to 80. The study was confined in three vegetation type viz., teak mixed, salmixed and mixed forest. In the study new approach was adopted by using ground data as well remote sensing data to get an accurate estimate of vegetation carbon pool in the region. Among the 30 selected site ID, Tectona grandis was dominated at 10 side. The basal area and volume of tree species in forest study sites ranged between 0.8 to 169.68 m2/ha and 0.11 to 2, 31299.82 m3/ha. The maximum tree biomass/ha recorded for Shorea roubsta...
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Posted by admin on Feb 28, 2017 in |
The emergence of novel antibiotics against the upsurge of “Superbugs” is a breakthrough health intervention. Daptomycin has gained prominence as a novel antibiotic produced by Streptomyces roseosporus which has demonstrated a broad spectrum of activity in vitro against a wide range of aerobic and anaerobic gram-positive bacteria, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci. The economic viability and large scale applicability of this high-value secondary metabolite is hampered by its poor production and tedious growth of S.roseosporus. The present study aims at strategic improvement of Daptomycin yield through reusability of the producer strain by its immobilization onto various non-conventional support matrices. Sufficient void volume, permeability, and cost effectiveness prompted the use of ultra porous refractory brick and ceramic foam for the immobilization of S.roseosporus. The surface area and porosity of the materials were characterized by BET surface analysis. Morphological transformations of the microbial cells were observed microscopically over the time. The cell mass adhered to the carrier was determined using SEM. The repeated use of immobilized cells led to...
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Posted by admin on Feb 28, 2017 in |
The objective of the study to formulate and evaluate of pulsatile drug delivery containing combination of domperidone and paracetamol for the treatment of migraine which is used to deliver the drug at specific time as per pathophysiological need of the disease and improvement of therapeutic efficacy and patient compliance. Domperidone and paracetamol in the core tablet was formulated with different concentration of superdisintegrants and microcrystalline cellulose, an outer shell tablet which is formulated with different weight ratios of ethyl cellulose (EC) and hydroxyl propyl methylcellulose (HPMC). The effects of the formulation of core tablet and outer shell of press coated tablets; on drug release and the lag time were investigated. The formulation was optimized based on acceptable tablet properties and in vitro drug release. The release profile of press coated tablet exhibited a lag time dependent upon the amount of HPMC K15 and EC in compression coating, followed by burst release. The optimized batch PRT3 gave a lag time of 6.1 h and drug release of 98.67% and 92.56%...
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