Posted by admin on Nov 30, 2016 in |
The objective of the present research work was to conduct in vivo pharmacokinetic study of microemulsion based transdermal gel of glimepiride for evaluating the enhanced bioavailability. Saturation solubility studies of the drug were conducted in various solvents and oils. Labrafil M 1944 CS, Tween 80 and Transcutol P were used as oil phase, surfactant and cosurfactants respectively for the preparation of microemulsion based on the results from solubility studies. Surfactant to cosurfactant ratio was fixed as 1:2 in all the formulations. Microemulsion based gel was prepared using carbopol 934 as gelling agent and oil to smix ratio of 1:9. In vivo pharmacokinetic studies conducted in rabbits revealed that the bioavailability of microemulsion based gel was increased 5.4 times compared to oral suspension demonstrating avoidance of first pass metabolism and oral degradation. This indicates the effective management of plasma profile of glimepiride when it is administered as microemulsion based gel through transdermal...
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Posted by admin on Nov 30, 2016 in |
A simple and precise method was developed for the assay of azelnidipine from tablet formulation. The solvent system and wavelength were optimized in order to maximize the sensitivity of the proposed method, azelnidipine shows the maximum absorbance at 257 nm. The separation was achieved on HPLC binary gradient system equipped with HPLC 3000 series. The mobile phase was prepared with Methanol: Water (80: 20%v/v) o-phosphoric acid used for the pH adjustment (pH-3). The method was validated for accuracy, precision, linearity, LOD & LOQ of sample solution. Linearity was observed in the concentration range of 20-100 µg/ml & gave mean correlation coefficient 0.998. The developed RP-HPLC method was found to be accurate, precise and was successful applied to a pharmaceutical tablet formulation for qualitative estimation of...
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Posted by admin on Nov 30, 2016 in |
Present study is a systematic approach to provide ‘proof of concept’ for effective oral delivery of Rosuvastatin lipid nanocarriers for treatment of hyperlipidemia. Rosuvastatin is a ‘Superstatin’ belonging to BCS class II having low bioavailability (20%). Development of lipid nanocarriers for oral delivery of rosuvastatin can be beneficial in enhancing bioavailability and providing sustained release. In the current work, Rosuvastatin lipid nanocarriers were developed using Precirol® ATO 5 as lipid carrier and Tween® 80as surfactant, employing solvent emulsification-evaporation method. Investigation of effect of shear rate, solution temperature and concentration of organic solvent as process parameters were studied. Three-level two-factor(32) experimental design was applied to study the effect of lipidand surfactant on percent entrapment efficiency and particle size. Complete characterization of optimized formulation such as particle size, zeta potential, entrapment efficiency, TEM and in vitro release profile was carried out. Lipid nanocarriers exhibited mean particle size 79.31±4.82 nm, entrapment efficiency 81.29±1.11% and zeta potential -6.89±3.29 mV. In vitro diffusion of ROS from lipid nanocarriers using dialysis bag diffusion method provided...
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Posted by admin on Nov 30, 2016 in |
Viral gastroenteritis is one of the major causes of childhood mortality in India. About 30 to 50 % gastroenteritis caused by unknown etiological agents. This study was aimed to identify unknown RNA viral etiology and their clinical manifestation with pediatric acute gastroenteritis, conducted at tertiary care hospital of Uttar Pradesh, North India from July 2012 to December 2013. A total of 234 patients with their clinical profiling were enrolled in this study. Identification of viruses was performed by serological (ELISA for rotavirus) and molecular methods (PCR for astrovirus, norovirus and cosavirus). Quantitative and qualitative data analysis performed by Chi-square and student t-test. Total subjects positive for infection was 35%.Co-infection present in 21% cases. Rotavirus, astrovirus, norovirus and cosavirus were detected in 25%, 10%, 7% and 3% subjects respectively. Other than diarrhea, vomiting was a significant symptom (p=0.0002). Severity of diarrhea was more than vomiting with 5.5±2.7 vs. 3.3±2.6 episodes in 24 hrs. Mean duration of symptoms 3.7±9.1. Eighty percent virus positivity were below the age of 6 years (39.2...
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Posted by admin on Nov 30, 2016 in |
The aim of the research work was to develop and optimize fast dissolving tablets of Promethazine Thecolate by direct compression using effervescent technology. For the investigation, purpose a 32 full factorial design was used to know the joint influence of two formulation variables, Crospovidone and Citric acid. The formulated tablets were evaluated for its percent friability and their disintegration time. The results of multiple linear regression analysis revealed that for obtaining a rapidly disintegrating dosage form, tablets should be prepared using an optimum concentration of sodium starch glycolate and a crospovidone. A contour plot was also presented to graphically represent the effect of the independent variables on the disintegration time 30 s and percent friability 0.5 %. A checkpoint batch was also prepared to prove the validity of the evolved mathematical model. The optimized tablet should be prepared with an optimum amount of Sodium starch Glycolate (2.75 mg), and Crospovidone (2.72 mg) which disintegrated in the 30 seconds, with friability of 0.5% and of drug release within 5 min....
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