Posted by admin on Dec 31, 2016 in |
The present study was aimed to evaluate the anti-oxidative defence system of Hydnocarpus laurifolia in diabetic rats and its potential effect against the β-cell damage induced by oxidative stress. Hydnocarpus laurifolia is a plant which belongs to the family Flacourtiaceae. According to the Indian system of traditional medicine it is used in the treatment of diabetes mellitus. Also literature reveals that the plant possesses anti-inflammatory, anti-leprotic and anti-oxidant property. Type II diabetes was induced in Wistar rats (180-250 gms) by intra peritoneal injection of Streptozotocin (STZ) at 30 mg/kg body weight. Diabetic rats were orally administered with ethylacetate extract of H.laurifolia (EEHL) at two different doses of 100 and 300 mg/kg body weight. Serum glucose and insulin levels were determined. The in vivo anti-oxidant study was determined by evaluating enzymatic anti-oxidants such as Superoxide dismutase (SOD), Lipid peroxidase (LPO) and Catalase (CAT) in hepatic tissue of STZ induced diabetic rats.The MDA levels were depleted and SOD and CAT enzymes were restored in diabetic rats treated with EEHL at the...
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Posted by admin on Dec 31, 2016 in |
Bryophytes- the small, slow growing group of plants is stockroom of naturally occurring materials. They contain a high number of biologically active compounds, however their use as a natural fungicides is negligible. In the recent years bryophytes has emerged as a potential biopharming tool for production of complex biopharmaceutiticals. In order to evaluate potential use of bryophytes as an antifungal compounds their basic chemical contents and the secondary metabolite profile was determined. Among secondary metabolites flavonoids are a group of polyphenolic compounds, which are widely distributed throughout the plant kingdom. Flavonoids like Rutin and quercetin possess many biochemical effects. An HPLC method was developed for the estimation of quercetin from methanol extract of Philonotis revoluta bryophyte. The results of the phytochemical screening using different extracts revealed the presence of alkaloids, flavonoids, phenols, etc. in the moss plant and investigated against plant pathogenic fungi using different parameters. Results showed significant reduction in pathogenic...
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Posted by admin on Dec 31, 2016 in |
For the present study thirty male Swiss albino mice were selected from an inbred colony. They were divided into three groups consisting of ten mice in each group. Group I: Sham exposed, Group II: 10 GHz MW (microwave) exposed, Group III: PDE (Prunus domestica extract) +MW exposed. After 30 days of treatment the animals were sacrificed to study alterations in body weights and biochemical parameters in different organs of Swiss albino mice viz. intestine, liver, testis and spleen. MW exposure did not resulted in significant reduction in body weights compared to sham exposed group of mice. PDE supplementation prior MW exposure did not result in any significant weight gain compared to MW exposed group. Biochemical analysis showed highly significant (p<0.001) variations in Lipid Per Oxidation (LPO), Glutathione (GSH) and protein levels which could be ameliorated by supplementation of PDE prior to MW exposure. Exposure to 10 GHz leads to biochemical alterations in different organs studied in mice which can be ameliorated by PDE supplementation. It can be concluded from...
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Posted by admin on Dec 31, 2016 in |
Background: Due to Escherichia coli O157:H7 bacteria’s peculiar biochemical characteristics it is considered an emerging pathogen. The aim of this study is to design, develop and evaluate the efficacy of a single formulation combining adsorptive and anti infective properties in the treatment of diarrhoea caused by Escherichia coli O157:H7 in an animal model. Method: A bilayered tablet of metronidazole and activated charcoal (AC) formulated via direct compression was developed using hydrophilic mucoadhesive polymer xanthan gum in varying concentrations to ensure modified release of metronidazole in the first layer and activated charcoal with microcrystalline cellulose for instant disintegration in the second layer. Escherichia coli infected Sprague Dawley® rats was utilized to evaluate the efficacy of the formulation. Results: Swelling studies reflected an affinity for the polymer to swell in pH 6.8 where drug release and swelling was influenced by ionic strength of the medium owing to the conformational changes in the xanthan gum in this media with an increase in the zeta charge from -28.5 mV to -40.2 mV, drug...
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Posted by admin on Dec 31, 2016 in |
The aim of present study is to formulate and optimize the site specific controlled release formulation for release of loratadine in upper gastrointestinal tract. Superposrous hydrogel hybrids (SPHH) were prepared by using ferric chloride as crosslinking agent and sodium carboxymethyl cellulose as composite agent. The Superporous Hydrogels were evaluated for swelling ratio, mechanical strength, density, porosity, scanning electron microscopic studies. Statistical software, Design Expert was used for optimization and chosing the final formulation. Loratadine hydrochloride was loaded in optimized formulation and characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry and in vitro drug release studies. Superporous Hydrogel Hybrids were prepared with desired mechanical strength and sufficient swelling ratio. Equilibrium Swelling Ratio of hydrogels followed the pattern SPHH < SPHC < CSPH. The pattern may be attributed to the additional crosslinking and decreased poresize. Reduced swelling ratio may be because of restricted polymeric chain flexibility. H- bonds between poly (AM-co-AA) and NaCMC reduced the polymer ability to form H-bonds with water molecules, limiting water absorption. Increase in...
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