Posted by admin on Nov 30, 2016 in |
A simple and precise method was developed for the assay of azelnidipine from tablet formulation. The solvent system and wavelength were optimized in order to maximize the sensitivity of the proposed method, azelnidipine shows the maximum absorbance at 257 nm. The separation was achieved on HPLC binary gradient system equipped with HPLC 3000 series. The mobile phase was prepared with Methanol: Water (80: 20%v/v) o-phosphoric acid used for the pH adjustment (pH-3). The method was validated for accuracy, precision, linearity, LOD & LOQ of sample solution. Linearity was observed in the concentration range of 20-100 µg/ml & gave mean correlation coefficient 0.998. The developed RP-HPLC method was found to be accurate, precise and was successful applied to a pharmaceutical tablet formulation for qualitative estimation of...
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Posted by admin on Nov 30, 2016 in |
Present study is a systematic approach to provide ‘proof of concept’ for effective oral delivery of Rosuvastatin lipid nanocarriers for treatment of hyperlipidemia. Rosuvastatin is a ‘Superstatin’ belonging to BCS class II having low bioavailability (20%). Development of lipid nanocarriers for oral delivery of rosuvastatin can be beneficial in enhancing bioavailability and providing sustained release. In the current work, Rosuvastatin lipid nanocarriers were developed using Precirol® ATO 5 as lipid carrier and Tween® 80as surfactant, employing solvent emulsification-evaporation method. Investigation of effect of shear rate, solution temperature and concentration of organic solvent as process parameters were studied. Three-level two-factor(32) experimental design was applied to study the effect of lipidand surfactant on percent entrapment efficiency and particle size. Complete characterization of optimized formulation such as particle size, zeta potential, entrapment efficiency, TEM and in vitro release profile was carried out. Lipid nanocarriers exhibited mean particle size 79.31±4.82 nm, entrapment efficiency 81.29±1.11% and zeta potential -6.89±3.29 mV. In vitro diffusion of ROS from lipid nanocarriers using dialysis bag diffusion method provided...
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Posted by admin on Nov 30, 2016 in |
Viral gastroenteritis is one of the major causes of childhood mortality in India. About 30 to 50 % gastroenteritis caused by unknown etiological agents. This study was aimed to identify unknown RNA viral etiology and their clinical manifestation with pediatric acute gastroenteritis, conducted at tertiary care hospital of Uttar Pradesh, North India from July 2012 to December 2013. A total of 234 patients with their clinical profiling were enrolled in this study. Identification of viruses was performed by serological (ELISA for rotavirus) and molecular methods (PCR for astrovirus, norovirus and cosavirus). Quantitative and qualitative data analysis performed by Chi-square and student t-test. Total subjects positive for infection was 35%.Co-infection present in 21% cases. Rotavirus, astrovirus, norovirus and cosavirus were detected in 25%, 10%, 7% and 3% subjects respectively. Other than diarrhea, vomiting was a significant symptom (p=0.0002). Severity of diarrhea was more than vomiting with 5.5±2.7 vs. 3.3±2.6 episodes in 24 hrs. Mean duration of symptoms 3.7±9.1. Eighty percent virus positivity were below the age of 6 years (39.2...
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Posted by admin on Nov 30, 2016 in |
The aim of the research work was to develop and optimize fast dissolving tablets of Promethazine Thecolate by direct compression using effervescent technology. For the investigation, purpose a 32 full factorial design was used to know the joint influence of two formulation variables, Crospovidone and Citric acid. The formulated tablets were evaluated for its percent friability and their disintegration time. The results of multiple linear regression analysis revealed that for obtaining a rapidly disintegrating dosage form, tablets should be prepared using an optimum concentration of sodium starch glycolate and a crospovidone. A contour plot was also presented to graphically represent the effect of the independent variables on the disintegration time 30 s and percent friability 0.5 %. A checkpoint batch was also prepared to prove the validity of the evolved mathematical model. The optimized tablet should be prepared with an optimum amount of Sodium starch Glycolate (2.75 mg), and Crospovidone (2.72 mg) which disintegrated in the 30 seconds, with friability of 0.5% and of drug release within 5 min....
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Posted by admin on Nov 30, 2016 in |
Vitex negundo is an Indian medicinal plant used to cure several diseases such as asthama, fever, headache, cancer and many other diseases. This study aims to determine the antioxidant potential and anti-proliferative activity of Vitex negundo methanolic leaf extract. Isolation of bioactive compounds involved liquid-liquid extraction of methanolic leaf extract by petroleum ether and chloroform. The total antioxidant potential of the extracts was determined using phosphomolybdenum, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric oxide reducing power (FRAP) and Nitric Oxide methods. It was observed that the crude and chloroform extracts had good antioxidant activity. The antileukemic activity of the chloroform extract was tested on HEL92.1.7 and Jurkat cell lines (Acute lymphoblastic leukemia derived) by cytotoxicity assay (MTT). The plant extracts exhibited poor cytotoxicity against the erythroid cell lines but showed good cytoxicity on Acute lymphoblastic leukemia cells. This study shows that the chloroform fraction of V negundo leaf has both antioxidant and antileukemic...
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