Posted by admin on Oct 31, 2016 in |
Fertility control is an issue of global challenge. Researchers are trying to develop male herbal contraceptives from natural sources. Jussiaea repens L (JR) is such an herb which is reported to have non toxic antigonadal activity in male rats. The present study has designed to evaluate whether crude queous extract of JR affects the DNA integrity of spermatozoa and also to study whether its actions are reversible or not. In this study, toluidine blue (TB), acridine orange (AO) and aniline blue (AB) staining were used to assess sperm chromatin / DNA integrity and comet assay for sperm DNA damage. Results show that the DNA integrity or denaturation by TB, AO and AB positive staining of spermatozoa of JR treated group were significantly increased when compared with control. But TB positive staining was much higher (34.51%) than AO (27.06 %) and AB (18.91%) positive staining. But no denaturation was observed in epididymal spermatozoa of rats after withdrawal of extract treatment. Results of Comet assay also support the reduced change in...
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Posted by admin on Oct 31, 2016 in |
The preliminary phytochemical evaluation and effect of Honey on Hematological parameters were investigated. Preliminary phytochemical screening was carried out to determine the presence of various bioactive constituents through different Tests. The Antimicrobial study of different Honey in combination with synergistics was carried out on selective microorganisms through well diffusion method. The effect of Honey on hemoglobin, WBC, Blood Sugar and weight loss was studied on swiss albino male mice under controlled conditions for 21 days. The Highest percentage of coumarins (+++), Resins (+++) were found in all the types of tested Honey among the different phytochemicals Screened. However the absence of carboxylic carbon and tannins were noticed during the study. The antimicrobial studies along with different synergistics shown significant results like Natural Omani Honey exerted 36mm zone of inhibition against S.aureus, while Turkish honey found to be more antimicrobial against P. aeruginosa (40mm). In the present study on Hematological and physiological parameters it was observed that increase in WBC % with greatest significance, among all the tested Honey with...
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Posted by admin on Oct 31, 2016 in |
The nutritional supplements are the natural food supplements, and other related products used to boost the nutritional content of the diet. These nutritional supplements have been of interest to research because of their antioxidant property by consumption of food and drinks. The present study deals with a comparative evaluation of antioxidant and anti-inflammatory effects of marketed nutritional supplements using cytoprotection, lipidperoxdiation and membrane stabilization activities on RBC under in-vitro condition. The marketed nutritional supplements exhibited cytoprotection or prevented hemolysis in erythrocytes and provided better protection against H2O2 induced oxidation and lipid peroxidation and exhibited good membrane stabilization when compared to standard. The Women Horlicks showed significant protection against H2O2 induced cytotoxicity in erythrocytes when compared to other supplements, the nutritional supplement Boost provided better protection against lipidperoxidation induced by H2O2 in erythrocytes. The nutritional supplement Maltova showed better membrane stabilization activity when compared to standard and other nutritional...
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Posted by admin on Oct 31, 2016 in |
The antidyslipidemic and antioxidant activities of Allium sativum (Garlic) and Aloe vera (Ghee- kuwar) extracts have been studied in two models of hyperlipidemia, triton and cholesterol rich high fat diet induced hyperlipidemia. A. sativum, A. vera and Gemfibrogil were macerated with 0.2% aqueous gum acacia and the suspension was fed orally to rats of group III, IV and V at a dose of 400mg, 1g/Kg, and 50 mg/Kg (b.w.p.o.) respectively with triton. Serum lipids were found to be lowered by Allium sativum and Aloe-vera in triton induced hyperlipidemia. On the other hand chronic feeding of these extracts to animals in cholesterol rich high fat diet induced hyperlipidemia for 30 days caused lowering in lipid and protein levels of β lipoproteins followed by an increase in α lipoproteins. The results of present study demonstrate that hypolipidemic activity of these extracts mediate through inhibition of hepatic lipid synthesis, increased faecal bile acid excretion and induced plasma LCAT activity in rats. Furthermore these extracts (100 and 200 µg/ml) inhibited the in vitro...
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Posted by admin on Oct 31, 2016 in |
The objective of the study is to measure the difference between the levels of LDL by Direct method versus Friedewald equation. The study includes 30 patients and 30 controls in the age of 25 – 75 years of both sexes. Fasting blood samples were collected and estimated Total Cholesterol (TC), Triglycerides (TG), Low Density Lipoprotein (LDL-C) cholesterol and High density lipoprotein (HDL) cholesterol, LDL cholesterol by direct method and by Friedewald’s formula (FW). There is a significant difference between LDL Direct and LDL-FW at triglyceride range of 1 – 100, (p= 0.01), 201 – 300 (p= 0.01) and no significant difference (p= 0.9) at 101 – 200, (p= 0.3) at 301 – 400 and (p= 0.2) at >400. There is a significant difference between LDL Direct and LDL-FW (p= 0.01) at total cholesterol range of 200 – 249 and no significant difference (p= 1.0) at 100 – 149, (p= 1.0) at 150 – 199 and (p= 0.9) at >250. There is significant difference between LDL values by direct and...
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