Posted by admin on Nov 30, 2016 in |
A new RP-HPLC method was developed for Mephenesin (MEP) and Ibuprofen (IBU) and validated as per ICH guidelines. Good chromatographic separation of Mephenesin and Ibuprofen was achieved by using Agilent C18 column (150mm×4.6mm, 3.2µ p.s). The system was operated at ambient temperature using a mobile phase consisting of Acetonitrile, 0.01M potassium dihydrogen phosphate pH 3.0 (60:40 v/v) isocratically at a flow rate of 0.5 ml/min. Detection was carried out at 212 nm and retention time was 3.4 min and 9.3mins respectively. Linearity was achieved from 10-40 µg/ml for Mephenesin and 2-8µg/ml for Ibuprofen with r2>0.99. The analytical method validation studies were performed as per International Conference on Harmonization-Quality (ICH-Q2 (R1)) guidelines. The method was efficiently validated with acceptable accuracy, specificity and precision for the estimation of Mephenesin and ibuprofen. It also aimed to apply the developed and validated method, for the analysis of drug release studies in marketed formulation (gel X) containing Mephenesin and...
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Posted by admin on Nov 30, 2016 in |
The present study is an attempt to formulate and evaluate cocrystals of poorly water soluble drug Darunavir by cooling crystallization method using succinic acid as coformer. Cooling crystallization method used to prepare the cocrystals of darunavir with succinic acid. The prepared cocrystals were characterized by Microscopic characters, Product yield, Surface morphology. (SEM), Differential scanning calorimetry, X-ray diffraction, Fourier Transform Infrared Spectroscopy, Micromeretic properties, Dissolution study of cocrystals, stability studies, Thus, cocrystals using water soluble polymer like Succinic acid can be prepared using cooling crystallization method for a poorly water soluble drug such as...
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Posted by admin on Nov 30, 2016 in |
The antimicrobial effects of I. balsamina seed hexane extract against Staphylococcus aureus, Klebsiella pneumonia, Proteus vulgaris, and Serratia marcescens were determined using the standard technique. As well as the phytochemical properties of the extract on the test isolates were also examined using the standard methods. The phytochemical components of the hexane extract of the I.balsamina seed include falvonoids, alkaloids, phenol, tannin, alkaloids, steroid, saponin, phenol, flavonoids, triterpenes glycosides and carbohydrate. All the test organisms were susceptible to 25mg/ml of the extract. The MIC and MBC of the hexane extract of the I.balsamina against S. aureus and E. coli was 50mg/ml, while that of K. pneumonia and S. marcescens were 75mg/ml and 100mg/ml of the extract respectively. The result of this study suggests that the hexane extracts of I. balsamina could be suitable for the treatment of diseases infections caused by S. aureus, K.pneumonia, P. vulgaris and S....
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Posted by admin on Nov 30, 2016 in |
Metal nanoparticles are usually synthesized using various chemical methods such as chemical reduction, Solvo-thermal reduction, electrochemical techniques and photochemical reaction in reverse micelles. All these methods are divided into two categories chemical and green chemical. Each having certain advantages and disadvantages. In this study we have tried to give a few examples of these methods for the synthesis of Titanium dioxide nanoparticles and further discussed to compare the...
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Posted by admin on Nov 30, 2016 in |
Aim of the current study was to evaluate the efficacy of non polar extract (NPE) of Aloe vera gel (AVG) for 60 days (~25μg/daily/orally) in rodent model of polycystic ovarian syndrome (PCOS). After treatment, steroid hormones and insulin levels were estimated by ELISA, serum lipid profile along with ovarian steroidal enzyme activity. Also, transcript as well as protein level of key receptors and steroidogenic enzymes were evaluated. Parallely, pairs of ovaries were excised and stained with Hematoxylin and Eosin (HE) to monitor the structural changes from group of animals. During course of study, stability of non polar extract (NPE) of AVG was evaluated by High Performance Thin Layer Chromatography (HPTLC) during study (15, 30, 45, 60 days). PCOS rats after treatment with NPE demonstrated a decrease in serum testosterone and insulin level with improved estradiol and progesterone levels. Also, decrease in transcripts level of steroid receptors while increased aromatase expression were observed after NPE treatment. These genes expression study was correlated with relative changes were observed with protein expressions....
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