Posted by admin on Feb 28, 2017 in |
Mononuclear ruthenium(II) complexes of the type [Ru(bpy)2(L)] (PF6)2, where L=4,5-bis(4-dimethyaminophenyl)-1H, 1 ‘H-2,2′-biimidazole (1) and 4,5-bis (4-fluorophenyl) – 1H, 1′ H-2,2′-biimidazole (2) have been synthesized and characterized by elemental analyses and spectroscopic techniques such as UV-visible, IR and ESI-MS. The redox behavior of the complexes has been studied by cyclic and differential pulse voltammetry. Both the complexes show reversible electrochemical wave attributable to Ru(II)/Ru(III) couple. The complexes exhibit half-wave potential (E1/2) values of +0.8698 V for the complex 1 and +0.8395 V for the complex 2 respectively. Electrophoretic studies showed that the complexes are able to cleave supercoiled plasmid DNA more effectively in the presence of light at the wavelength of 480 nm. The mode of action of DNA cleavage activity was studied using DMSO and histidine. DPPH method has been adopted to find the radical scavenging activity of the newly synthesized ligands and complexes. The cytotoxic activity of these complexes was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against MCF7 breast cancer cell...
Read More
Posted by admin on Feb 28, 2017 in |
Ruellia species belong to Acanthaceae family, and are perennial creepers with widespread medicinal uses including analgesic and anti-inflammatory activity. The objective of this study was to determine the qualitative phytochemical constituents and anti-oxidant activity of crude extracts from whole plant parts of Kenyan Ruellia species viz. Ruellia prostrata. Whole parts of the plants were collected, air-dried under shade and organic extraction was done by cold maceration using ethyl acetate and methanol. Aqueous extraction was done by boiling. Anti-oxidant activity was performed based on the ability of the aqueous, methanolic and ethyl acetate extracts to scavenge free radicals produced by 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Ascorbic acid was used as the standard. Anti-oxidant tests were done at eight concentrations (3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 and 500 µg/ml). The anti-oxidant activity of both plant extracts and ascorbic acid increased with increase in concentration. Methanolic and ethyl acetate extracts exhibited a higher anti-oxidant activity with IC50 values of 20.58 and 22.26 µg/ml respectively, relative to aqueous extract (IC50 of 51.92µg/ml). Ascorbic acid standard...
Read More
Posted by admin on Feb 28, 2017 in |
Skin aging is a complex process induced by constant exposure to ultraviolet (UV) irradiation and damages human skin. Rambutan (Nephelium lappaceum), a delicious tropical fruit that grow in Malaysia and spread widely in most Southeast Asian countries, have strong antioxidants properties due to the presence of ellagic acid, corilagin, geraniin, β-carotene and vitamin C. These compounds scavenge the free radical and thereby protect the skin against oxidative damage. The aim of the present study is to formulate and evaluate an antiaging cream containing Rambutan fruits extract. The SMEP, SMEF, CMEP and CMEF produced significant antioxidant activities and tyrosinase inhibition with low IC50 values. All the four extracts were formulated into an antiaging cream and evaluated. The results showed that the formulated antiaging creams and its ingredients were consistent in quality and can be easily used. From the above results, it is concluded that the formulation containing SMEF and CMEF are safe and usable for the skin. The present results demonstrate that the Rambutan fruits extracts has a good potential...
Read More
Posted by admin on Feb 28, 2017 in |
Herbal medicine is the oldest form of health care known to humanity and has been used in all cultures throughout history. The validation of these novel products needs powerful analytical devices tailored for the study of herbal extracts in order to assess composition and face their natural complexity as a resource. The last item is important and crucial for the capacity and utility of the analytical results that means that each product should be analyzed with the right approach. Having in mind these arguments, the present study was selected HPTLC as useful tool for the analysis. The present study is mainly focused to establish the fingerprint profile of Tragia plukenetii R. Smith using high performance thin layer chromatography (HPTLC) and Preliminary phytochemical screening. Preliminary phytochemical screening of the extract showed the presence of alkaloids, triterpenes, phenolic compounds and steroids. HPTLC finger printing of chloroform extract of leaf revealed 15 peaks with Rf values in the range of 0.11 to 1.0. It can be concluded that HPTLC fingerprint analysis of...
Read More
Posted by admin on Feb 28, 2017 in |
Objectives: The study aimed at evaluating the broncho-relaxant effect of the test drug Flupirtine in experimentally induced Asthma in Guinea pigs & compares its efficacy with the Standard. Materials and Methods: Guinea pigs of both sexes weighing around 350-400 gm were randomly divided into 3 groups of 6 animals each. Groups were categorised into Control, Standard and test groups. Animals serving as controls received 10 ml/kg body weight of Normal saline; Standard group received 4.5 mg/kg body weight of salbutamol & the test group received the test drug flupirtine in doses of 46.5 mg/kg of body weight. All the compounds were administered once a day, per orally, through a gavage tube for 5 days. On day 5 experimental bronchial asthma was induced by exposing animals to 0.25% histamine acid phosphate under constant pressure (40mm/Hg) in an aerosol chamber. Animals exposed to histamine aerosol developed progressive dyspnoea. Pre-convulsive time (PCT) was determined from the time of exposure to onset of dyspnoea, immediately before the appearance of convulsions. Greater the PCT...
Read More
