Posted by admin on Oct 31, 2016 in |
A novel stability-indicative of RP-HPLC method was developed and validated for the quantitative estimation of Empagliflozin in bulk drugs and pharmaceutical dosage form in the presence of degradation products. Chromatographic separation was achieved on an Intersil C18 (150mmx4.6mm, 5 μm) analytical column using mobile phase composition of methanol and acetonitrile in ratio of (50: 50 v/v) that was set at a flow rate of 20μl/min with detection of 265 nm. The retention time of Empagliflozin was found to be 2.184min. The high correlation coefficient value indicated clear correlation and their peak area within the LOQ (Limit of quantification) to 150% level. The drug was analyzed by following the guidelines of International conference on Harmonization (ICH) underneath hydrolytic, photolytic, oxidative, and thermal stress conditions. The presentation of the method was validated according to the present ICH guidelines for accuracy, precision and robustness, Linearity, limit of quantification, limit of detection...
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Posted by admin on Oct 31, 2016 in |
Obesity is very serious and concerned problem these days. From the first human civilization, research is going to find the drugs to treat obesity and its complications. Despite availability of many drugs in market to treat obesity, no single drug is ideal for treating all sorts of problems caused by obesity. So the research is going on finding perfect drug. Prior going to evaluating drugs on humans, it is necessary to go for preclinical evaluation and usually the rodents are suitable models. The ideal obesity models available for obesity are induced by using chemicals and high fat diet. Methanolic extract of aerial parts of Desmostachya bipinnata plant was studied for its Anti-obesity activity in animal experimental models. Wistar albino rats, albino mice were used to study anti-obesity activity of methanolic extract of D.bipinnata plant aerial parts at doses 200 mg/kg p.o. and 400 mg/kg p.o. against the standard orlistat 50 mg/kg p.o. in models of anti-obesity activity viz. High fat induced obesity, Progesterone induced obesity model. The induction of...
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Posted by admin on Oct 31, 2016 in |
In this present study a novel, simple, economical, accurate and precise RP-HPLC method has been developed and validated for simultaneous estimation of aspirine and clopidogrel in combined tablet dosage form. Chromatographic separation was carried out on a Kromasil HD C18 (150 x 4.6 mm, 5µm) column with a mixture of acetonitrile and 0.1% (v/v) orthophosphoric acid aqueous solution in a ratio of 40:60 (v/v) as mobile phase at a flow rate of 1.5 ml/min. Detection was accomplished at 237 nm by employing a DAD (Diode array detector). The retention times were 2.78 and 4.99 min for aspirine and clopidogrel respectively. The method was validated according to ICH guidelines for accuracy, precision, linearity, specificity and sensitivity. Calibration plots were linear over the concentration range 10 – 70 µg/ml for both aspirine and clopidogrel with r2>0.997. Recovery was found to be 99.2 – 99.8% and 99.2 – 99.9% for aspirine and clopidogrel respectively with RSD < 2 for both. There were no interference due to any components of pharmaceutical dosage indicating...
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Posted by admin on Oct 31, 2016 in |
The cytotoxic effect of safranal, a pharmacologically active component of saffron, has been established in vitro. The aim of this study was to develop safranal nanoliposomes with a higher therapeutic index for the treatment of cancer. Thus, various PEGylated safranal nanoliposomes were prepared using HSPC and cholesterol by solvent evaporation. The liposomes were characterized by their size, in vitro cytotoxicity and in vivo therapeutic efficacy against C26 tumor bearing mice. Liposome characterization illustrated the size range of 140-230 nm and PDI of 0.2-0.3. The entrapment efficiency was considerably low due to the high instability of safranal in liposomes, causing a substantial in vitro release. In vitro cytotoxicity indicated higher toxic effects of safranal liposomes compared to free form. Treatment of tumor-bearing mice with selected safranal liposomes (50 mg/kg) did not improve the tumor size and survival of animals compared to controls. These results were presumably due to the physicochemical properties and dose dependent effects of safranal molecules. In addition, the intensive hydrophobic molecular interaction between safranal and cholesterol within...
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Posted by admin on Oct 31, 2016 in |
Paracetamol (acetaminophen) (PA) is a widely used as an analgesic and antipyretic drug that is commonly available in without a prescription. Paracetamol has been used to treat many diseases such as headache, muscle aches, arthritis, backache, toothaches, colds, and fevers. It cures pain in mild arthritis but has no effect on the underlying inflammation and swelling of the joints. It has been reported as the common cause of drug toxic ingestion. These studies aim to compare the effect of some Paracetamol derivatives such Phenacetin, p-nitroacetanilide, p-bromoacetanilide and n-acetylanthranilic acid on liver structure to avoid Paracetamol cancer effect during its metabolism. From the histological point of view, it has been found that p-nitroacetanilide is the best alternative due to its metabolite...
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