Posted by admin on Nov 30, 2016 in |
The aim of the present study was to investigate the production of laccase by Marasmius sp. BBKAV79 in Solid State Fermentation (SSF) and its antiproliferative activity. Experiments were also conducted to determine the variables that could affect activity of laccase. In particular, the effects of the initial moisture content, pH, temperature, and inoculum volume, incubation period in addition to carbon and nitrogen sources were evaluated. Rice bran was found to be the best supported lignocellulosic substrate for extracellular laccase production under SSF. Highest activity of laccase was achieved at pH 6.0 and temperature at 40°C. Inoculum size and incubation period for laccase production at 2000µl culture broth and 10 days respectively. The presence of starch and ammonium sulphate in the growth medium was found to be favourable for the production of the laccase. Such a high activity was obtained without any addition of inducers. Thus, the indigenous isolate is found to be a potential producer of laccase using SSF and can be exploited for further biotechnological applications. The process...
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Posted by admin on Nov 30, 2016 in |
Introduction and Objectives: Oral submucous fibrosis (OSMF) is a chronic, progressive, and irreversible disease of the oral mucosa. Though numerous modalities have been tried but all are palliative and have no curative value. Pentoxifylline possessing vasorelaxive and antifibrotic properties believed to potentiate the anti-inflammatory, anti-oxidant and immune modulating properties of garlic. So, the present preliminary clinical trial was undertaken to evaluate the efficacy of Garlic in conjunction with pentoxifylline in the management of OSMF. Methods: The study consisted of 15 OSMF patients. Pentoxifylline (400 mg) was administered thrice daily along with garlic pearls, 2 pearls; thrice daily. Drugs were administered systemically after food for a duration of 3 months. Patients were evaluated every 15 days for the assessment of clinical parameters, i.e. burning sensation, reduction in mouth opening, tongue protrusion and cheek flexibility. Pre and post treatment SOD level were measured and compared. The improvement was assessed at the end of the study. Results: All the patients were males with the mean age of 26.04 years. Patients exhibited a...
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Posted by admin on Nov 30, 2016 in |
Aim: The present study has been undertaken to investigate the therapeutic effect of GSE (Grape seed extracts) against Tamoxifen (TAM), induced hepatotoxicity in rat. Methods: The rats were divided into four groups • Group 1: rats were injected intraperitoneally (i.p.) with saline for seven days. • Group 2: Rats were treated with TAM in a dose of 45 mg/kg b·w/day, i.p., for seven successive days. • Group 3: Rats were administrated orally GSE (100 mg/kg b·w/day) for three weeks. Group 4: rats were injected (i.p.) (45 mg/kg b·w/day) of Tamoxifen for seven days, then treated daily with a single dose of GSE (100 mg/kg b·w/day) for three weeks respectively. Results: GSE reduced necrosis in the TAM-treated rat. And significantly increased (p<0.05) the levels of MDA and PCC, while the level of GSH was significantly (P<0.05) decreased. Treatment with GSE significantly (P<0.05) reduced. Tamoxifen significantly decreased (P<0.05) the level of NO. GSE significantly increases (P<0.05, Table 2) NO level. Flowcytometric analysis in liver cells, significant increases in apoptotic cells treated...
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Posted by admin on Nov 30, 2016 in |
Aim: To study the relationship between serum Vitamin D levels and insulin resistance in obese and non obese adolescents. Introduction: Vitamin D deficiency in obese adolescent is strongly associated with increased risk for diabetes, hyper tension and metabolic syndrome. Vitamin D insufficiency is a risk factor for developing impaired glucose in childhood obesity and is associated with insulin resistance in obese adolescents. In our study, we examined the relationship between Vitamin D and insulin resistance in obese adolescents. Materials and Methods: The study group included 50 obese adolescent aged (17-19 years) and compared with 50 age and gender matched healthy controls. Anthropometric data were collected and fasting plasma glucose was estimated by (GOD-POD) method, serum Insulin was estimated by (FEIA) method and insulin resistance was calculated by using (HOMA-IR) and serum Vitamin D was measured by using ELISA method. Results: The Vitamin D levels in obese adolescents is slightly lower than the controls. The insulin levels in obese adolescents is slightly higher than controls. Insulin resistance was significantly higher...
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Posted by admin on Nov 30, 2016 in |
Rosuvastatin is a Dyslipidaemic agent, which acts by inhibiting HMG-CoA reductase enzyme and used in the treatment of hyperlipidemia. But Rosuvastatin calcium (RST) exhibit unsatisfactory dissolution profiles, problems of absorption and poor bioavailability. Thus objective of the study is to increase the solubility and dissolution rate of Rosuvastatin calcium (RST), a poorly water-soluble 3-hydroxy 3-methyl glutaryl CoA (HMG-CoA) Reductase inhibitor through inclusion Complexation with β-cyclodextrin (β-CD). Therefore the present investigation was to design a formulation of orally disintegrating tablets of Rosuvastatin. Orally disintegrating tablets of Rosuvastatin were formulated by superdisintegrants addition method by direct compression technique. Formulas prepared by direct compression showed good results, the prepared inclusion complex with β-CD by kneading method exhibited greatest enhancement in solubility and fastest dissolution (99.363 % RST release in 45 min, 102.22 % RST release in 50 min.) of RST. The inclusion complex contains RST: β-CD (1:1) was formulated into tablets using super disintegrants like sodium starch glycolate, crosspovidone and crosscarmellose. The prepared tablet were evaluated for various post compression parameters like...
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