Posted by admin on Oct 31, 2016 in |
Objective: Diabetes mellitus is metabolic disorder and day by day number of people increased across the world. The aim of the present study was to evaluate the antidiabetic and heapatoprotectivity of ethanolic extracts of Coriandrum sativum in alloxan induced diabetic rat and find out the harmonious activity of the extract. Methods: The alloxan (150 mg/kg, i.p) used for induction of diabetes after making diabetic model the ethanolic extract of Coriandrum sativum leaves used at a dose of 200 and 400 mg/kg b.w. respectively for single extract treatment for four weeks including metformin (200mg/kg b.w) as a reference drug. The statistical analyses were carried out by one- way ANOVA with post-hoc by Dunnett’s Multiple Comparison Test. Results: After 28 days treatment the extracts significantly (p<0.001) reduces serum glucose level and recovered liver function tests (p<0.001) (SGOT, SGPT, ALP and bilirubin) as well glycosylated haemoglobin (p<0.001). Histopathological study showed amelioration in alloxan treated groups. Conclusions: The result suggested that anti-antidiabetic and heapatoprotectivity potential of Coriandrum...
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Posted by admin on Oct 31, 2016 in |
Alkaline protease alone account for 60% of the total world enzyme market with its improved properties such as higher optimum temperature and alkaline pH. Alkaline Lonar Lake, situated in the Buldhana District of the Maharashtra State, India, is a unique ecosystem and harbors various haloalkaliphilic bacterial species which produces biotechnologically important thermo-haloalkaliphilic enzymes. The present study deals with the production and partial characterizations of enzyme protease isolated from the extremophilic Lonar Lake bacterium. A total six bacterial strains were isolated on Horikoshi medium from the water, sediment and matt samples collected from Lonar crater. Out of these, one bacterium showing prominent proteolytic activity was characterized on the basis of morphological, cultural and biochemical parameter and identified as Bacillus halodurans (AJ302709) by16S rRNA sequencing. The alkaline protease produced by Bacillus halodurans showed optimum activities at pH 8, at temperature 700C. It proves that the enzyme alkaline protease produced by this bacillus has potential applications in food, pharmaceutical and detergent...
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Posted by admin on Oct 31, 2016 in |
The synthesis of a novel of pyrazole derivatives was achieved by condensation of acetyl furan with phenyl hydrazine to give hydrazone (1) On the other hand, cyclization of α, β-unsaturated ketone. Using Vilsmeier reagent by DMF (dimethylformamid) and POCl3 Phosphorusoxychloride) to give compound (2). The chemical structures of allnew compounds were established by IR, ¹HNMR, and mass spectra data. All the synthesized compounds were screened for in vitro antibacterial activity and most of them showed potency against both gram positive and gram negative bacteria. Compounds 4-(α-benzoyl aminoacrylic acid) – 3 – Furayl-1-phenylpyrazol, 4 – (α-benzoylaminomethylacrylate)-3-Furayl-1-phenylpyrazol, 4- (2-4-dinitrophenylhydrazone)-3-Furayl-1-phenylpyrazole showed the highest antibacterial activity against Bacillus subtilis strain with minium inhibition zone 19...
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Posted by admin on Oct 31, 2016 in |
The influence of fluconazole pre-treatment on the hypoglycaemic effect of pioglitazone (10 mg/kg, p.o) and rosiglitazone (720µg/kg, p.o) was studied. This study was conducted on diabetic rats of either sex, randomly distributed into two different groups. Diabetes was induced by administering 120mg/kg of alloxan intraperitonially. Then they were pretreated with fluconazole (36 mg/kg) in 2% w/v gum acacia suspension for seven days. On eighth day, rosiglitazone and pioglitazone were administered to respective groups one hour after fluconazole treatment. Blood samples were collected by retro orbital plexus under light ether anesthesia at different time intervals up to 24 hours. The hypoglycaemic activity of thiazolidinediones was studied and it indicated that fluconazole pretreatment has enhanced the hypoglycaemic effect of pioglitazone and rosiglitazone, at higher dose, significantly. Hence it is suggested that during the concomitant usage of fluconazole and thiazolidinediones, the therapeutic drug monitoring is essential and may also require readjusting the dose and frequency of administration of...
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Posted by admin on Oct 31, 2016 in |
The present study was carried out to evaluate oral hypoglycemic activity of rhizome of Hemidesmus indicus R.Br. in Albino wistar rats. Hemidesmus indicus (Family: Asclepiadaceae) is a commonly known Indian Medicinal Plant. It contains various phytoconstituents like hemidesminine, hemidine, hemidesmin-1 and hemidesmin-2 and other chemical constituents like glycosides, flavonoids, tannins, sterols and volatile oils. Diabetes mellitus (DM), commonly referred to as diabetes, is a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. Streptozotocin induced diabetic albino wistar rat by administrating graded oral doses (200 and 400 mg/kg b.wt.) injected to group 3 and 4 for 90 days. This study also includes histopathological studies of pancreas. Phytochemical screening of methanol rhizome extract of Hemidesmus indicus R.Br. The presence of alkaloids, saponins, cardiac glycosides, tannins and steroid. Metformin (100mg/kg) as standard oral hypoglycemic agent for 12 weeks resulting in significantly reduction in blood glucose. The present research work indicated that Hemidesmus indicus R.Br. methanol extract of rhizome (HIME) have prominent antidiabetic effect at a dose of...
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