Posted by admin on Aug 31, 2016 in |
There are various studies on growth of Streptococcus mutans which was done by different scientists on different media but it has been found that there is still some deficiency in existing medium. In our study we have tried to prepare a modified, less laborious and more selective medium for Streptococcus mutans which is specific for their growth. We studied 10 plaque samples from caries active school children and 2 reference strains of Streptococcus mutans ATCC 25175 (serotype c) and OMZ 61 (serotype a) to evaluate the selectivity and sensitivity of our modified medium i.e TSBB (Thioglycolate Sucrose Blood Bacitracin) agar. To check efficiency of our modified TSBB medium, both reference strains and plaque samples were plated on TSBB and other four old existing media i.e TYCSB, TSY20B, MM10, and GSTB for their comparison and evaluation. In our study it was found that highest recovery of Streptococcus mutans was observed on TSBB medium followed by TYCSB medium. TSBB medium inhibits the all Streptococcus species other than Streptococcus mutans present in...
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Posted by admin on Aug 31, 2016 in |
To investigate the anti-arthritis effects of hydro-alcoholic stem bark extract (HSBE) of Plumeria rubra in Complete Freund’s adjuvant (CFA) induced arthritis model in wistar albino rats. Paw edema was produced by sub-plantar injection of 0.1 ml of complete Freund’s adjuvant (CFA) and paw volume was measured with plethysmometer. HSBE of the plant P. rubra (250 mg/kg & 500 mg/kg body weight) was administered orally for 14 days. Arthritic assessment was carried out based on parameters including paw edema, body weight, and spleen index. At the end of study period, animals were sacrificed and various biochemical, oxidative stress parameters, radiological and histological parameters were evaluated. Administration of HSBE significantly attenuated the behavioral, biochemical, hematological, radiological alteration induced by the CFA in dose dependent manner. From our study, we can conclude that the hydro-alcoholic extract of the plant has the potent activity to check the arthritis edema as well as inflammation & body weight. The potency of ant-oxidant activity was managed significantly. The radiological and histopathological investigations revealed that the extract...
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Posted by admin on Aug 31, 2016 in |
The antioxidant and anti-inflammatory activities of various solvent extracts of Fomitopsis dochmius, a polypore found in Western Ghats of Kerala, India were investigated. Petroleum ether (PE), ethyl acetate (EA), 70 % aqueous ethanol (ETH) and hot water (AQ) extracts of the fruiting bodies of the mushroom were examined for their antioxidant activities. PE was found to show activity against hydroxyl radicals, lipid peroxidation and ABTS.+ radicals. EA extract was able to scavenge DPPH., hydroxyl, and ABTS.+ radicals and also showed ferric ion reducing and lipid peroxidation inhibition properties. ETH and AQ extracts were active against all the above mentioned radicals besides superoxide and nitric oxide radicals. Since ETH extract showed higher antioxidant activity than other solvent extracts, its anti-inflammatory property was examined. The extract, at a dose of 500 mg/ kg body weight, was found as effective as the standard anti-inflammatory drug diclofenac (10 mg/ kg body weight). The preliminary phytochemical analysis of the four extracts indicated that the fruiting body of the mushroom contained steroids, terpenes, coumarins, phenols,...
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Posted by admin on Aug 31, 2016 in |
Very little knowledge about use of papaya leaves is available in the literature, the leaves of papaya are bitter to taste which explains its therapeutic benefits. The present study was undertaken to assess anti diabetic effects of leaves extracts of Carica Papaya and Glucophage on streptozotocin –induced diabetic mice. Results indicates that hyperglycemic level in diabetic mice was normalized after administration of various doses (50, 150 and 300 mg/kg bw) of ethanolic leaves extracts to diabetic mice. Similarly leaves extracts of C. Papaya have also shown pronounced effects on plasma insulin, triglyceride, cholesterol and HDL cholesterol levels. The levels of liver enzymes (ALT, AST and ALP), bilirubin and blood cells (Red cells, white cells and platelets) were improved on dose depended manner. Analysis of various leaves extracts for phytochemicals revealed presence of significant amount of alkaloids, steroid, tannins, total phenols and flavonoids. It is assumed that biologically active components of papaya leaf extracts are helpful in decreasing secondary complications of diabetes and improved insulin sensitivity to uptake glucose by...
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Posted by admin on Aug 31, 2016 in |
Enhanced production of anthraquinones from cell suspension cultures of Oldenlandia umbellata were established through elicitor or precursor treatment. Stock cell suspension cultures were developed in liquid MS medium supplemented with 2.5 µM NAA using friable calli. Standardization experiments of suspension cultures revealed that 10 µM NAA added liquid MS medium was optimum for the growth and AQ accumulation from O. umbellata suspension cultures. 10 µM NAA produced 9.96 mg g -1dw AQ on 30th day of incubation. Later enhanced AQ production was achieved by the addition of elicitors or precursors into the standardized media on 25th day of incubation and AQ quantification was done after 5 days of incubation. Addition of elicitors or precursors resulted in a sudden increase in AQ content. 25 mg L-1 pectinadded suspension cultures produced 35.67 mg g-1dw AQ and precursor feeding with 50 mg L-1α-keto glutaric acid resulted in the accumulation of 42.63 mg g-1dw AQ. HPLC analysis of elicitor or precursor mediated suspension cultures revealed the presence of alizarin and purpurin in samples....
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