Posted by admin on Sep 30, 2016 in |
The aim of the present study is to carry out fluorescence analysis, phytochemical extraction, preliminary phytochemical screening, estimation of total flavonoids, tannins, alkaloids, steroids saponins and in vitro antiurolithiatic studies on the leaf aqueous and ethanolic extracts of Bauhinia variegata. The results of fluorescence analysis indicate that the powder on treatment with 50% H2SO4 shows dark brown colour under UV. The results of preliminary phytochemical screening indicated the presence of saponin glycosides, tropane alkaloids and acidic compounds. The results of quantitative determination indicated that the aqueous extract contains highest amount of flavonoids expressed as 54.6 mg/gm equivalents of quercetin, maximum amount of tannins in ethanolic exract as 56.30 mg/gm equivalents of quercetin, equal amounts of alkaloids are present in both extracts as 25mg/gm equivalents of atropine sulphate, steroids and saponins in lowest amount. The in vitro antiurolithiatic activity was studied as percentage inhibition of stones by nucleation, growth and aggregation assays for aqueous and ethanolic extracts at 200-1000 µg/ml taking cystone tablets as standard. The results indicated that the...
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Posted by admin on Sep 30, 2016 in |
Background: Many factors contribute for prevalence of high blood pressure including poor treatment compliance, lack of access to health care and lack of physician adherence to therapeutics. In Ethiopia, routine health care report is incomplete and erratic and 90% individuals diagnosed with hypertension do not properly adhere to treatment. The present study was carried out to assess the antihypertensive drug utilization pattern in Butajira Zonal Hospital, Ethiopia. Methodology: A retrospective cross-sectional study was conducted on 486 patient cards of hypertensive patients from Jan, 23 2011 – Feb. 03, 2011 in Butajira Zonal Hospital, SNNPR, Ethiopia. Results: The hypertensive patients studied were female 267 (54.94%) and 271 (55.76%) were above 45 years of age. The common signs/symptoms and disease reported were headache 31(19.50%), congestive heart failure and urinary tract infection 23(14.46%). Combination therapy study on 275 patients shows 237(86.18%) and 38(13.82%) were on two and three drugs respectively. A total of 211 anti-hypertensive drugs were prescribed as mono-therapy out of them hydrochlorothiazide was mostly 123(58.29%) prescribed. A total of 198(100%)...
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Posted by admin on Sep 30, 2016 in |
Dillenia indica linn fruit, commonly known as elephant apple has indigenous use in various culinary dishes of Assam and it has a history of being used as traditional medicine. The present study was undertaken to evaluate its antioxidant and chemopreventive properties. Ethanolic extract of Dillenia indica fruit was used as experimental sample. Antioxidant activity was studied in vitro, at concentrations (20µg/ml, 40µg/ml ,60µg/ml, 80µg/ml, 100µg/ml) where it showed significant (p<0.05) inhibition of free radicals formation, hydroxyl radical and nitric oxide scavenging property and also reducing ability when compared with a standard antioxidant, ascorbic acid. Chemopreventive potential was evaluated in 8-10 weeks old swiss albino mice by giving a single tropical application of initiator- 7,12-dimethylbenza(α)anthracene (DMBA) at a concentration of 100µg/100µl acetone and promotion after 14 days by repeated application of croton oil (1% in acetone) thrice a week for 15 weeks. Oral application of D.indica at dose of 250 mg/kg body weight of mice selected after acute oral toxicity test, showed significant (p<0.05) reduction in DMBA induced mice skin...
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Posted by admin on Sep 30, 2016 in |
Bromhexine hydrochloride, mucolytic agent used in the treatment of respiratory disorders. Azithromycin is use in wide variety of bacterial infections. The aim of this present work is to formulate dual drug delivery system containing azithromycin as immediate release and bromhexine hydrochloride as sustained release action. The sustained release part as core tablet of bromhexine prepared because of the frequent daily dosing is required for therapy. The floating formulation of core tablet was prepared because it dissolved in the pH range of 1 to 4. The core tablets of bromhexine hydrochloride were prepared by wet granulation technique. The prepared core tablets were evaluated for different parameters for selection of formula for floating the tablets. The core compressed tablets were evaluated for quick release of coat layer containing disintegrants. The various formulations of core tablets were prepared using HPMC K4M and K15M in different concentration. The prepared tablets were evaluated for physical parameters, disintegration time, floating lag time and drug release study. All the batches show around 70 sec disintegration time,...
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Posted by admin on Sep 30, 2016 in |
Asthma is obAdd Newstructive disease characterized by bronchoconstriction and inflammation for which beta 2 (β2) agonists that act through the β2 adrenoceptor are the first line agents of choice. Glycine16Arginine (Gly16Arg) is a common single nucleotide polymorphism (SNP) of β2 adrenoceptor whose effects on treatment response remain inconclusive. Hence the study aims to understand and determine the effect of the Gly16Arg SNP on β2-agonist binding and treatment responsiveness. Structure guided mutagenesis was performed with discovery studio tool, energy minimization was performed using Chemistry at Harvard Macromolecular Mechanics(CHARMM) force field, protein confirmation was studied using Ramachandran plot, Molecular docking analysis was performed using Autodock 4.2 and statistical analysis was performed using Graph pad prism 6.0. Statistically significant difference was observed between the binding energies of Glycine16 (Gly16) and Arginine16 (Arg16) groups. Further, the binding energies of β2 agonists were found to be comparatively less in Arg16 group than the Gly16 group suggesting that the Arg16 variant carriers may be poor responders of β2 – sympathomimetic therapy. Presence of functional non...
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