Posted by admin on May 31, 2016 in |
Kaempferol and quercetin are the flavonoids which are widespread in fruits and vegetables exhibiting important biological activities. A simple and precise HPTLC method was developed and validated for simultaneous determination of kaempferol and quercetin in herbal drug extracts of Podphyllum hexandrum and Cassia angustifolia. The HPTLC of flavonoids was performed with toluene: acetone: formic acid (7:3:0.25) as mobile phases on RP-18 F254 TLC plates. Densitometric determination of flavonoids was performed at λ = 254 nm in reflectance/ absorbance mode. The results obtained indicated that the developed method was precise and accurate. Good resolution of kaempferol and quercetin was obtained from other constituents present in methanolic and hydrolysed extracts of P. hexandrum and C. angustifolia using proposed HPTLC method. Kaempferol was quantified in free (8.01and 2.42 µg/mg) as well as bound (17.54 and 8.13 µg/mg) forms and quercetin in free (4.5 and 0.93 µg/mg) as well as bound form (6.17and 4.8 µg/mg) in P. Hexandrum and C. angustifolia. Kaempferol was found to be present in high concentration and quercetin was...
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Posted by admin on May 31, 2016 in |
Fingerlings of Mystus (M.) vittatus (Bl.) a freshwater siluroid when subjected to a sublethal concentration of 2.4 mg/l hexavalent chromium for 20 days elicited significantly decreased RNA and protein in the liver. However, 10 days of exposure under the same stress produced no significantly alteration neither in the protein content nor in the nucleic acids (DNA, RNA) content of the liver. Results are discussed in relation to pollutional stress of hexavalent...
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Posted by admin on May 31, 2016 in |
All the newly synthesized Coumarin derivatives have shown considerable anti-inflammatory activity. In the present research work, we synthesized a series of novel Coumarin derivatives. The Coumarin derivatives such as substituted 7-hydroxy-4-methyl-2H-chromen-2-one and substituted 6-chloro-7-hydroxy-4-methyl-2H-chromen-2-one were synthesized from substituted benzoyl chloride. The chemical structures of the synthesized compounds were confirmed by means of IR and 1H-NMR. These compounds were screened for anti-inflammatory activity by carrageenan induced paw edema method in rats at a dose of 30 mg/kg body weight. All the tested compounds of Coumarin derivatives were found to be more potent when compared to standard drug...
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Posted by admin on May 31, 2016 in |
This study aimed to develop and optimize a multi-particulate formulation for colon targeted delivery of rifaximin by application of a functional coat of Eudragit L100 and Eudragit S100 mixture on the drug layered pellets. The rifaximin-excipients compatibility was confirmed with FTIR studies. Functional coat completely restricted drug release in pH 1.2 dissolution medium. Statistical optimization of the formulation variables, i.e., ratio of Eudragit L100 and S100, and % of coating, in respect to drug release at 2nd and 8th h in pH 6.8 dissolution medium was conducted with a central composite design and response surface methodology. Compared to % of coating, the impact of polymer ratio was more pronounced for modulating the drug release. Comparatively, higher influence of % coating was observed after 2 h of dissolution. As per graphical optimization, the minimum drug release at 2nd h and maximum drug release at 8th h could be achieved in formulation with 3.3 polymer ratio and 15.4 % coating where more than 80 % of drug should be delivered in...
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Posted by admin on May 31, 2016 in |
Peptide deformylase (PDF) is a mononuclear metal ion protein that is responsible for removing the N-formyl group of nascent proteins found in bacteria and chloroplasts in order for them to become mature proteins. From literature it was found that heterocyclic derivatives containing hydroxamic acid and hydroxyl amine exhibit good antibacterial and PDF inhibitory activity. Heterocycles bearing nitrogen, sulphur, oxygen and thiazole moieties constitute the core structure of a number of biologically interesting compounds. Recently 1,3,4 oxadiazole and 1,3,4 –triazole derivatives received significant attention owing to their diverse range of biological properties particularly being antifungal, antitubercular, antibacterial, antiviral, anticancer and antioxidant. Ethyl-cyano- acetate when treated with sodium hydrate and THF give acetate derivatives, which on further treatment with hydrazine hydrate and various reagent afforded heterocyclic hydroxylamine...
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