Posted by admin on Oct 31, 2016 in |
Ischemic heart disease (IHD) is the major cause of morbid-mortality in most countries. The involvement of oxidative stress (OS) in the development of atherosclerosis, ischemic cardiopathy triggering phenomen, has been investigated. Serum total homocysteine (Hcy) concentration was measured by fluorescence polarization immunoessay. Plasma levels of substances reacting with thiobarbituric acid (TBARS) were carried out by fluorimetric method (Yagi). Erythrocyte activity of superoxide dismutase (SOD) and plasma total antioxidant status (TAS) were determined by a colorimetric method at 505nm and 600nm, respectively. Erythrocyte activity of catalase (CAT) was measured by colorimetric assay (Góth). Glutathione peroxidase (GPx) and the glutathione reductase (GR) erythrocyte activities were determined by a colorimetric method at 340 nm. Our study showed significant elevation of Hcy and TBARS levels in patients compared to controls. While we noted significant decrease in TAS levels, SOD, CAT, GPx and GR activities in patients compared to the control group. We also noted positive correlations between: Hcy-TBARS, SOD-TAS, CAT-TAS, GR-TAS, GPx-TAS, SOD-CAT and GPx-GR. However negative correlations between: Hcy-SOD, Hcy-CAT, Hcy-GPx, Hcy-GR,...
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Posted by admin on Oct 31, 2016 in |
Objectives: To describe the prevalence, severity, impact and treatment aspects of dysmenorrhoea in females of reproductive age group in tertiary care teaching hospital. Material and Methods: This was a questionnaire based survey type study. Detail information regarding demographic profile, menstrual history and dysmenorrhoea was analysed. Severity of dysmenorrhoea was evaluated by a Menstrual Distress questionnaire (MDS). Visual analogue scale (VASP) was used to measure pain. Analysis was done by mean, SD and Chi square. Results: Out of 350 females, 198 (56.57%) females had dysmenorrhea. Dysmenorrhoea was observed more common in unmarried females (93.43%) and in age group of 15-19 year (48.28%). Regularity of menstrual cycle had no statistically significant association with dysmenorrhea. Most common domain of MDS score was Pain (92.93%) followed by Negative affect (71.71%), Behavioural change (61.11%) and concentration (54.54%). By VAS score, severity of pain was mild to moderate in majority of females (82.33%). Only 27.78% females had taken treatment. Conclusion: Dysmenorrhea is a very common problem and affects quality of life in reproductive aged females....
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Posted by admin on Oct 31, 2016 in |
A novel stability-indicative of RP-HPLC method was developed and validated for the quantitative estimation of Empagliflozin in bulk drugs and pharmaceutical dosage form in the presence of degradation products. Chromatographic separation was achieved on an Intersil C18 (150mmx4.6mm, 5 μm) analytical column using mobile phase composition of methanol and acetonitrile in ratio of (50: 50 v/v) that was set at a flow rate of 20μl/min with detection of 265 nm. The retention time of Empagliflozin was found to be 2.184min. The high correlation coefficient value indicated clear correlation and their peak area within the LOQ (Limit of quantification) to 150% level. The drug was analyzed by following the guidelines of International conference on Harmonization (ICH) underneath hydrolytic, photolytic, oxidative, and thermal stress conditions. The presentation of the method was validated according to the present ICH guidelines for accuracy, precision and robustness, Linearity, limit of quantification, limit of detection...
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Posted by admin on Oct 31, 2016 in |
Obesity is very serious and concerned problem these days. From the first human civilization, research is going to find the drugs to treat obesity and its complications. Despite availability of many drugs in market to treat obesity, no single drug is ideal for treating all sorts of problems caused by obesity. So the research is going on finding perfect drug. Prior going to evaluating drugs on humans, it is necessary to go for preclinical evaluation and usually the rodents are suitable models. The ideal obesity models available for obesity are induced by using chemicals and high fat diet. Methanolic extract of aerial parts of Desmostachya bipinnata plant was studied for its Anti-obesity activity in animal experimental models. Wistar albino rats, albino mice were used to study anti-obesity activity of methanolic extract of D.bipinnata plant aerial parts at doses 200 mg/kg p.o. and 400 mg/kg p.o. against the standard orlistat 50 mg/kg p.o. in models of anti-obesity activity viz. High fat induced obesity, Progesterone induced obesity model. The induction of...
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Posted by admin on Oct 31, 2016 in |
In this present study a novel, simple, economical, accurate and precise RP-HPLC method has been developed and validated for simultaneous estimation of aspirine and clopidogrel in combined tablet dosage form. Chromatographic separation was carried out on a Kromasil HD C18 (150 x 4.6 mm, 5µm) column with a mixture of acetonitrile and 0.1% (v/v) orthophosphoric acid aqueous solution in a ratio of 40:60 (v/v) as mobile phase at a flow rate of 1.5 ml/min. Detection was accomplished at 237 nm by employing a DAD (Diode array detector). The retention times were 2.78 and 4.99 min for aspirine and clopidogrel respectively. The method was validated according to ICH guidelines for accuracy, precision, linearity, specificity and sensitivity. Calibration plots were linear over the concentration range 10 – 70 µg/ml for both aspirine and clopidogrel with r2>0.997. Recovery was found to be 99.2 – 99.8% and 99.2 – 99.9% for aspirine and clopidogrel respectively with RSD < 2 for both. There were no interference due to any components of pharmaceutical dosage indicating...
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