Posted by admin on May 31, 2016 in |
A study on biological monitoring of simultaneous exposure to benzene and alcohol was made in petrol pump operators of Meerut city (India). In addition to alcohol, other confounding factors viz. cigarette smoke, food habit, age and work experience were also considered. Thirty two, 20-40 years old male workers were registered for this study. Respective urine samples collected after the workshift were analyzed for specific gravity, creatinine and phenol. Phenol concentration corrected to creatinine was found to be low (22.22 mg/g creatinine) in alcoholic pump operators in comparison to non-alcoholic operators (24.09 mg/g creatinine). It has been suggested that acute ethanol ingestion inhibits benzene oxidation in the liver with an ethanol inducible form of CYP450. Although urinary phenol concentration in the registered Indian petrol pump operators is less than BEI as suggested by ACGIH (50mg/g creatinine), simultaneous exposure to alcohol and benzene may potentiate the hepatotoxicity, cytotoxicity of benzene amongst petrol pump...
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Posted by admin on May 31, 2016 in |
A method using derivative UV spectrophotometry was developed and validated for the determination of methylxanthines and tannins in guarana bulk, using caffeine and catechin as markers. The spectra were obtained using first order derivatization, Δλ=20.000, and scale factor of 10. The quantification of actives was performed using wavelengths of 244 and 277 nm for catechin and caffeine, respectively. The parameters of specificity, linearity, precision, accuracy, robustness, limits of detection (LOD) and quantification (LOQ) were established to validate the method. The method was shown to be specific for quantification of methylxanthines and tannins, and linear over the concentration range 5–35 µg mL-1, with a correlation coefficient of r=1(caffeine) and r=0.9993 (catechin). The results indicated good intra-day and inter-day precision with RSDs less than 2%. The method was accurate, exhibiting recoveries between 98 and 102%. The LODs were 0.184 µg mL-1 (caffeine) and 0.368 µg mL-1 (catechin), and the LOQs were 0.552 µg mL-1 (caffeine) and 1.104 µg mL-1 (catechin). Methylxanthines and tannins were quantified, and contents of 10 µg mL-1...
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Posted by admin on May 31, 2016 in |
The availability of several brands of Simvastatin tablets in Libyan pharmacies today places health practitioners in a problem of generic substitution. The aim of the present study was the evaluation and comparison between four different Simvastatin brands, which are commercially available in the Libyan market produced by various pharmaceutical companies with different trade names. The physicochemical equivalence of four brands of Simvastatin tablets was investigated through the evaluation of both official and non-official standards according to the USP pharmacopoeia including uniformity of weight, thickness, hardness, disintegration time, drug content as well as dissolution rate. Acceptable external features as well as uniformity in diameter and thickness revealed for all the tablets. The entire brands complied with the official specifications for uniformity of weight where no tablet showed a deviation more than ±7.5%. Brand B had the highest crushing strength and highest disintegration time compared to the other brands. All the brands had values within the range specified for assay in the BP. The dissolution profiles showed that none of the...
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Posted by admin on May 31, 2016 in |
An experimental study was carried out after getting approval from institustional animal ethical committee to study the role of oral iron chelator, deferasirox in prevention of myocardial ischemic reperfusion injury in albino rabbits. Albino Rabbits of either sex were divided into two groups control (n=5) and test group (n=5), receiving deferasirox. Experiments were done using isolated heart perfusion apparatus (Langendorff apparatus). The experiment was divided into three phases, perfusion (15 min) followed by ischemia (10 min) followed by reperfusion (15 min). LDH level of perfusate and coronary flow were taken as biochemical and physiological marker of myocardial reperfusion injury respectively and were measured at 5, 10 and 15 min of post ischemic period. Control and test groups were compared. The result shows that in the group receiving oral iron chelator, deferasirox, the decrease in post ischemic LDH level was significant (p< 0.01) at 5, 10 and 15 min while there was significant increase in post ischemic coronary flow at 5 min (p< 0.01) as compared with control. Thus oral...
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Posted by admin on May 31, 2016 in |
Amino acid chelated complexes were synthesized and characterized to determine their purity. The purity of the complexes was found to be ranging from 42-75%. The HPLC method for determination of free amino acid content in the complexes was developed and validated and found to be linear, precise and accurate within concentration range of 0.157 mg/mL to 20.14 mg/mL. The method was used to estimate the purity of the amino acid complexes. The changes in the carboxylate and amine group stretches in FT-IR indicated the association of metal ion with the amino acid. The initial studies described above demonstrated that the chelated complexes can produce sufficient osmotic pressure to be used as ergogenic...
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