Posted by admin on Jun 30, 2016 in |
This paper describes simple, sensitive, accurate, rapid, precise and economical Chemometrics assisted and RP-HPLC methods for simultaneous estimation of Linezolid(LNZ) and Cefuroxime axetil(CEF) in bulk and tablet. Chemometrics offers a useful approach for an estimation of combined dosage form. The measurement carried out at wavelength 240 to 290nm with interval of 4nm for Linezolid and Cefuroxime axetil respectively. Chemometrics method were found to be linear (R2=0.999) for all four methods CLS, ILS, PLS and PCR for LNZ and CEF respectively. HPLC method by using mobile phase Acetonitrile: water 65:35 with Thermo Synchronies C-18 (250mm x 4.6mm, 5μm) flow rate 0.8ml/min and detection wavelength 267nm. Retention time was found to be 3.573 min for LNZ and 4.273 min for CEF. HPLC method was found to be linear (R2=0.999) for 6-30µg/ml for LNZ and 5-25µg/ml for CEF respectively. The applied methods Chemometrics and RP-HPLC were found to be successful for simultaneous estimation of LNZ and CEF in marketed dosage...
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Posted by admin on Jun 30, 2016 in |
Objective: Present study was aimed to evaluate the total phenolic, flavonoid content and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of whole plant of Catharanthus pusillus. Methods: Antioxidant activity have been tested using various antioxidant model systems viz., DPPH, hydroxyl, superoxide, ABTS and reducing power. Results: Methanol extract of Catharanthus pusillus is found to possess higher DPPH, hydroxyl, superoxide radical scavenging activity, while ethyl acetate extract is found to possess higher ABTS radical cation scavenging activity. Methanol extract of whole plant of Catharanthus pusillus shows the highest reducing ability. Conclusion: The study indicates the significant free radical scavenging potential of Catharanthus pusillus whole plant which can be experimented for the treatment of various free radicals mediated...
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Posted by admin on Jun 30, 2016 in |
Ayurveda is the primeval complete serving system in medical field. However, one of the barriers in the acceptance of the Ayurvedic formulation is the paucity of standard quality control outline. World health organization (WHO) in 1999 has given a detail procedure for the standardization of herbal drugs comprising of a single content but not for standardization of polyherbal formulations. Mahashankhvati is official in Ayurvedic Formulary of India and is prescribed for treatment of haemorrhoids, malabsorption syndrome, dyspepsia and indigestion. In the proposed work, attempt has been made for standardization of Mahashankh Vati by developing chromatographic method. Piperine from Piper longum and Piper nigrum, Umbelliferone from Ferula asafoetida and Gallic acid from Terminaliachebula present in formulation were selected as marker compounds. A new, rapid, simple, precise, selective HPTLC method was developed for marketed preparation of Mahashankhvati. The separation was performed on TLC aluminium plates precoated with silica gel 60 F254, using toluene: ethyl acetate: methanol: formic acid (7:2:2.5:0.5 v/v/v/v) as mobile phase. The densitometric analysis was carried out at the...
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Posted by admin on Jun 30, 2016 in |
Protein kinases are key players in cellular signal transduction and perturbations in its activity leads to cancer. Targeting these seems to be sufficient to provoke apoptotic response, thus limiting the process of tumorogenesis. Among kinase inhibitors, imatinib has demonstrated magnificent clinical efficacy. But patients with advanced disease harbor faces resistance. Currently there is requisite of making strategies to overcome resistance and adverse effects in order to provide maximum benefit to the patients. Present work was to develop a new analog of Imatinib with the objective to provide a molecule with best combination of properties, safe and affordable drug. For this purpose Imatinib was taken as a lead molecule which has shown a successful positive bioactivity score of 0.59 for kinase receptor predicted using Molinspiration software. Imatinib was optimized using the concept of Bioisosteric replacement, in order to achieving better affinity towards protein kinase receptor. Newly designed analogs were found in compliance with Lipinski rule of five recommendations for new chemical entity to have good oral bioavailability with no violations....
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Posted by admin on Jun 30, 2016 in |
Oral squamous cell carcinoma is one of the leading causes of mortality in India. The various haematological and biochemical changes including liver enzymes imbalance occur in cancer treatment. The careful monitoring of serum biochemical and haematological changes had very important role in prognosis of disease. Therefore, the present study aimed to study haematological and biochemical changes in pre and post treatment of buccal mucosa carcinoma patients. In study, totally 198 buccal mucosa carcinoma subjected were included from both genders. We observed the Haematological parameters (level of completer haemogram, differential leucocytes count), biochemical and liver function parameters in pre and post treatment of subjects. The serum biochemical and haematological levels were lower in post treatment groups than before treatment of subjects, whereas glucose level did not show significant changes. Hence, the subjects did not affected by diabetics. Most of subjects had anaemic, treatment severely affected bone marrow. However, these changes were exists statistically significant by paired samples t-test at p˂0.001. The biochemical and haematological changes were additional risk for subjects....
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