Posted by admin on Jun 30, 2016 in |
A simple, precise, accurate, reproducible and economical reverse phase liquid chromatography method was developed and validated for the quantitative simultaneous estimation of Bisoprolol Fumarate and Hydrochlorothiazide in bulk and marketed formulations. Estimation of drugs in this combination was done with a C18 column Kromasil 100-5C18 column [250mm x 4.6mm].using mobile phase of composition Acetonitrile and phosphate buffer (40:60 v/v, pH 3).The flow rate was 1 ml/min and the effluents were monitored at 228 nm. The retention time of Bisoprolol Fumarate and Hydrochlorothiazide were 3.3 min and 6.25 min respectively. The method was found to be linear over a concentration range of 20-100 mg/ml for both Bisoprolol Fumarate and Hydrochlorothiazide. The established method proved as reproducible one with a %RSD value of less than 2 and having the robustness and accuracy within the specified limits. Assay of marketed formulation was determined and find with 98.1% and 97.6% for Bisoprolol Fumarate and Hydrochlorothiazide respectively. The method was validated according to the guidelines of International Conference on Harmonization (ICH) and was successfully...
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Posted by admin on Jun 30, 2016 in |
The objective of the present investigation was to evaluate the antibacterial and antioxidant potential of n-hexane and methanolic extracts of Sterculia foeida L. bark. The antibacterial potential of Sterculia foetida L. bark was tested against human pathogens causing diarrhoea and dysentery such as Shigella flexneri (MTCC-9543), Salmonella enterica ser typhi (MTCC-733), Bacillus subtilis (MTCC-1305), Streptococcus mitis (MTCC-2897), Klebsiella pneumoniae (MTCC-109) and Staphylococcus aureus (MTCC-1430) by using agar well diffusion method. The result of the study revealed that n-hexane extract was highly effective against Shigella flexneri, whereas Klebsiella pneumoniae showed no response and Salmonella enterica ser typhi, Bacillus subtilis, Streptococcus mitis, Staphylococcus aureus responded moderately. The methnolic extract had high inhibition against Salmonella enterica ser typhi (15.16 ± 0.20 mm) whereas Shigella flexneri showed no response and moderate effect against other test bacteria. The antioxidant activity by DPPH scavenging method resulted in significant antioxidant potential of n-hexane and methanolic extracts with IC50 value of 51.26 and 66.84 respectively. Ciprofloxacin was taken as the reference...
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Posted by admin on Jun 30, 2016 in |
Medicinal plants are of important therapeutic aid for various treatments. Leucas cephalotes Spreng. up in cultivated fields as a weed, especially after a period of rain. In the present study the dried plant stems powder of Leucas cephalotes Spreng. was taken and subjected to extraction with ethanol and aqueous extraction was done on the marc left after ethanolic extraction. The antimicrobial activity in terms of zone of inhibition and minimum inhibitory concentration of different extracts of Leucas cephalotes Spreng.. was tested against three bacterial strains viz. Staphylococcus aureus, Escherichia coli, Vibrio cholera and two fungal strains viz. Candida albicans, Aspergillus niger. The zone of inhibition was determined for these pathogenic microorganism by using specific standards and respective controls for determining Minimum inhibitory Concentration (MIC). The ethanolic extract showed more activity against S. aureus and others bacterial/fungals strains than the aqueous extract. It was concluded that the ethanolic extract of the plant Leucas cephalotes Spreng. was more active against all the test strains than the aqueous...
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Posted by admin on Jun 30, 2016 in |
Combination drug formulations are better in terms of effectiveness and hence are used many times for treatment of diseases. The physico-chemical properties of the different API used in a formulation have a significant impact in the development of a single method for the analysis of such drugs. In the current research a rapid analytical method employing GC with Mass Spectroscopy detection has been developed and validated for simultaneous quantification of the active ingredients Ornidazole and Miconazole from the cream formulation. The analytes were extracted from cream base and filtered. A 95% dimethyl polysiloxane column with 5% phenyl polysiloxaneis used for the separation of the analytes. The method involves simple temperature gradient and MS detection. Validation of the method showed response was a linear function of concentration in the range 50-150 µg/mL for both Ornidazole and Miconazole. The method was suitably validated and was found to be precise and robust, with recoveries for both the analytes being consistent and complete. The method has been successfully applied for the analysis of samples...
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Posted by admin on Jun 30, 2016 in |
The present study describes the biochemical, antioxidant and anticancer properties of the crude and melanin free ink of Sepia prabahari which is rich in both organic and inorganic components. The quantitative analysis define the presence of protein (1.16 – 1.49 mg/ml), lipid (0.25 – 1.42 mg/ml), carbohydrates (0.01 – 1.14 mg/ml), ash (0.4 & 0.1%) and moisture (93.33%) content. The native PAGE profile showed distinct bands of protein with molecular weight ranging from 3.5 to 205 kDa. The ink is composed of different amino acids, lipids and sugars which was presented by TLC. The ink revealed good antioxidant activity, thereby lowering or retarding the initiation of lipid oxidation process. The ink was screened for its anticancer activity against human breast adenocarcinoma cell line (MCF 7) and it exhibited a strong cytotoxicity by inhibiting the cell growth. The results of comet assay revealed the DNA damage in the cells treated with ink which is a hallmark of apoptosis. Thus the ink, especially the melanin free ink is known to possess...
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