Posted by admin on Jun 30, 2016 in |
This research emphasis on microwave assisted extraction of fruits of Sapindus mukorrosai. Aqueous fruit extract is subjected for the isolation of saponins, focusing only on higher molecular weight of saponins using dialysis membrane. On treatment of aqueous solution of silver nitrate with isolated saponin, stable silver nanoparticles were rapidly formed. The green synthesized silver nanoparticles were characterized by UV-vis spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray (EDX). The SEM confirms the synthesis of square shape of nanocrystalline particles with the size range of 60-90 nm. The presence of saponins has confirmed by FTIR spectra. These nanoparticle has been subjected for hypolipidaemic activity in animal model. It showed significant reduction in total cholesterol, triglyceride level, HDL and VLDL...
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Posted by admin on Jun 30, 2016 in |
Antimicrobial peptides (AMP) showed a broad spectrum of cytotoxic activity against cancer cells, which could provide a new class of anticancer drugs. In this study, we have evaluated the cytotoxic and apoptotic activity of magainin II, an AMP compared with 5-Fluorouracil (5-FU) in colo320 DM cancer cells, and magainin II cytotoxicity was assessed on vero cell line by MTT. The cells cultured in the presence or absence of magainin II and 5-FU. The magainin II inhibited cell viability in dose dependent manner was quantified by MTT assay. The IC50 of magainin II was found to be 89.3nM and at this concentration the cytotoxicity was not observed in vero cell line. The cytotoxicity of the magainin II was further confirmed by lactate dehydrogenase (LDH) assay and neutral red uptake assay (NRU). Apoptosis induction was verified by DNA fragmentation analysis. The morphological changes in colo 320 DM cells was measured by DAPI and acridine orange/ethidium bromide staining, showed nuclear condensation, and this substantiates the apoptotic action of magainin II. The cell...
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Posted by admin on Jun 30, 2016 in |
The crude methanolic study of Vanda roxburghii root was investigated for antioxidant, analgesic and cytotoxic activity. The extract of Vanda roxburghii root showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging power assay. In DPPH and NO scavenging assay the extract shown adequate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 113.35±1.27 and 127.31±0.26ug mL-1 while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug ml-1. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1b.wt. doses displayed significant (p<0.001) reducing in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1b.wt. Which is comparable to the standard diclofenac sodium (86.52%). The extract was also investigated for cytotoxic activity using Brine Shrimp lethality bioassay. In this the extract showed...
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Posted by admin on Jun 30, 2016 in |
The best QSAR model were generated with left of adept and significant descriptors like electronic, lipophilic and topological, using multiple linear regression (MLR) and partial least square (PLS), model further explained by using forward feed neural network analysis (FFNN). QSAR is a kind of technique that directly correlates in between chemical structure to their biological activity. The best MLR statistical expressions were evaluated with good predictive and authenticated ability and the values were S =0.367, F =53.06 r =0.910, r² =0.828, r2(cv)=0.780. The r2 (training and test-set) values of MLR, PLS and FFNN are 0.82, 0.71, 0.82, 0.71 and 0. 81, 0.74 respectively, which predicts the soundness of the model. The model reveals that total dipole moment, bond lipole and kappa 3 are prerequisite descriptors for determining further promising GSK-3β antagonist with high and liable potency against target. In addition to QSAR modelling, Lipinski’s rule of five was employed on a series and we found no violation in it, which means 3-aryl- 4-(arylhydrazono) 1H pyrazol-5-ones has enough good pharmacokinetic...
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Posted by admin on Jun 30, 2016 in |
Objective: Conventionally, sustained release pellets have been prepared in the form of reservoir systems by coating non-pareil pellets which is an expensive and tedious process. The aim of present study was to develop sustained release matrix (SRM) pellets of Nateglinide (NT), an oral antidiabetic agent, commonly used in the treatment of Type 2 Diabetes mellitus. Methods: The SRM pellet formulations of NT were prepared with different polymers like HPMC K4, HPMC K15, Kollidon® SR (K-SR), Hydrogenated castor oil (K-HCO) by extrusion spheronization process. The SRM pellet formulations were characterized for micromeritics properties, aspect ratio, sphericity, friability, Kawakita analysis, Fourier transform infrared spectroscopy (FTIR), drug content and In vitro drug release studies. Results: The optimized formulation of SRM pellets (Batch F7) prepared by using K-SR (35%w/w) and K-HCO (15 %w/w) has shown desired sphericity and excellent micromeritics properties. The friability and drug content value were found in the range of 0.22- 0.81% and 96.8-99.7% respectively for all formulations. FTIR spectroscopy studies have not revealed any chemical interaction between the NT...
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