Posted by admin on May 31, 2016 in |
This study compares the performance of Mycobacterium Growth Indicator Tube (MGIT) culture against conventional Lowenstein Jensen (LJ) media and direct AFB smear examination in pulmonary and extra pulmonary tuberculosis specimens for isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-320) media at a tertiary care centre in North India A total of 132 consecutive clinical samples were obtained from suspected cases of TB for smear-microscopy and culture on LJ and BACTEC-MGIT-320. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol. A total of 132 specimens were cultured on LJ and the BACTEC-MGIT-320. The mean (range) time to detection was 14.2 (3–40) and 23.2 (8–42) days for MGIT-320 and LJ, respectively 69 (52.3%) were positive with either MGIT or culture of which 45 (65.2%) were smear positive and 24(34.8%) were smear negative. On comparing MGIT and LJ culture, 65 (94.2%) were positive with MGIT-320 whereas 62(89.8%) LJ cultures were positive DST was conducted on both MGIT-320 and LJ media in the 58 concordant culture-positive isolates. Thirty...
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Posted by admin on May 31, 2016 in |
The electrochemical behavior of Manidipine is studied by employing cyclic voltammetry (CV) and Differential Pulse Polaro graphy (DPP) in universal buffers of PH ranging from 2.0 to 12.0. The kinetic parameters, such as transfer co-efficient, diffusion co-efficient, and heterogeneous forward rate constant values are evaluated by employing these techniques. Differential Pulse Polarography is employed for the estimation of Manidipine in selected pharmaceutical...
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Posted by admin on May 31, 2016 in |
Introduction: Proteolytic enzymes like serratiopeptidase, trypsin and chymotrypsin are used for prolonged period for edema resolution in post-operative patients, arthritis, tooth extraction etc. either alone or with other anti-inflammatory agent. Various trials have supported and refused the role of enzymes as anti-inflammatory agents. No study was found for analgesic property of proteolytic enzymes. Aims: This study was designed to investigate the anti-inflammatory and analgesic activity of these three proteolytic enzymes alone and in combination with diclofenac at therapeutic doses. Methods and Material: Carrageenan induced paw edema was used for acute inflammation. Edema was measured with the help of plethysmometer at 0 hour and 3 hour. Modified formalin test was used to evaluate the analgesic property. 1% formalin was injected into the forepaw and scoring was done by appropriate and standard method. Statistical Analysis: Edema changes of hind paw and licking duration in seconds of a fore paw with different treatments were compared with one-way ANOVA followed by Tukey-Kramer multiple comparison test with the help of graph pad instat software....
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Posted by admin on May 31, 2016 in |
The extracellular alkaline phosphatase from halotolerant facultative alkaliphile Bacillus flexus FPB17 was purified through precipitation / dialysis / chromatography and resulted in 28 fold of purification. The molecular weight of this enzyme through SDS-PAGE gel is 63 ± 2 kDa. Optimum conditions for purified ALP activity were pH 9.0 and 35°C. The enzyme proved to be thermolabile and gets completely denatured at 60°C in 10 min. p-NPP was found to be a preferred substrate and Km (0.33 mM) and Vmax (0.2 mM mg-1) determined using p-NPP as a substrate. Inorganic phosphate proved to be a competitive inhibitor of ALP. Mg+2 and Ca+2 increased the ALP activity by 47% and 20% respectively whereas Sodium fluoride, Sodium arsenate and EDTA decreased the activity by 47%, 65% and 77% respectively. The superiority of dephosphorylation of λ phage DNA by this newly discovered alkaline phosphatase over that of calf intestinal alkaline phosphatase of M/s. HiMedia Laboratories, Mumbai opens up possibility of its future use in molecular biology as a replacement of alkaline phosphatases...
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Posted by admin on May 31, 2016 in |
We planned to clarify the association between the Vitamin D supplementation and predisposition of metabolic syndrome including obesity, diabetes mellitus, hypertension and dyslipidemia in arthritic patient with pre diabetic condition and to assess prediabetes interfere in the disease progression. Retrospective observational study was conducted on 236 patients. Of these, Arthritis patients with no co-morbid condition and not supplemented with Vitamin D (47), Arthritis patients supplementing with Vitamin D (63), Arthritis patients with prediabetic condition (68), Arthritic prediabetic patients supplementing with Vitamin D (58) were recruited based on inclusion and exclusion criteria. All anthropometric parameters and clinical findings were recorded the predominance of women with RA was observed in this study and the body weight was increased substantially in entire study group irrespective of gender. A significant increase in metabolic parameters was observed in patients with arthritis who were off of Vitamin D supplementation (p<0.05). On the other hand no such significant increase was observed in vitamin D supplemented group (p>0.05. In the group of arthritic patients with prediabetes condition...
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