Posted by admin on Apr 30, 2016 in |
Lipase is an important of extracellular thermostable enzyme that has received considerable attention for potential use in the food industry. Hence to achieve large scale production of gram positive and negative organism is commercially important lipase enzyme under the fermentation. Lipases are produced by microorganisms such as bacteria and fungi. However, we have focused on bacterial microbial lipases were economically importance of several properties. The present studies of the goals of this paper were isolation and identification of lipase producing bacteria from biogas producing power plant waste volatile substances as sources. Optimization of the experimental conditions for the maximal amount of enzyme production was...
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Posted by admin on Apr 30, 2016 in |
A simple, selective, rapid and economical High Performance Liquid Chromatography (HPLC) method was developed for estimation of Acyclovir in bulk and marketed formulation. Chromatographic separation and estimation was achieved in a run time of 6.482 minutes on Hemsil C18– 5U (250 × 4.6 mm, 5μm) using Acetonitrile and pH adjusted water (6.74) in the ratio of 10:90 v/v at flow rate of 0.5ml/min. UV detector set at 254nm was used for detection. The linearity range for Acyclovir was found to be 4-40 µg/ml with coefficient of linear regression greater than 0.9948. The method was validated as per International Conference on Harmonization (ICHQ2 (R1) 2005) guidelines for accuracy, precision, specificity, LOD & LOQ, linearity and robustness. The method was successfully applied for estimation of Acyclovir from bulk and marketed formulation for routine...
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Posted by admin on Apr 30, 2016 in |
In the present study, Gastro retentive floating drug delivery systems (GFDDS) of sitagliptin, an anti diabetic drug, have been designed to increase the therapeutic efficacy & gastric residence time and to reduce frequency of administration. Therefore a sustained release medication was advantageous so as to achieve the prolonged therapeutic effect and to reduce peak and valley effect in plasma concentration. This can be achieved by formulating modified gastro retentive sustained release dosage forms which resides in the stomach for sufficient time to release the drug in vicinity of the absorption zone. The tablets were prepared by direct compression method, by employing polymers like HPMCK100, Poly vinyl pyrrolidine and polyacrylic acid respectively in various concentrations. The prepared granules were evaluated for angle of repose, bulk density, tapped density, compressibility index and Hausner’s ratio and results obtained were satisfactory compressed formulations were further evaluated for thickness, friability, hardness, swelling index and in-vitro dissolution studies. All the formulations showed good results which were compliance with pharmacopoeial standards. In vitro dissolution study was...
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Posted by admin on Apr 30, 2016 in |
Fixed dose combination (FDC’s) is the term, which generically used to mean a particular combination of active ingredient irrespective of the formulation or brand. Now a day’s irrational FDCs are more rapidly being marketed, which results in serious ADRs and reduction of patient quality of life. The study aims at assessing the rational usage of fixed dose combinations in community pharmacies by collection and evaluation of FDCs by using seven-point assessment scale, and development of FDC education tool for practicing rational use. The six months prospective interventional study, carried at community pharmacies where different FDCs were collected. All data regarding demographics details were collected in a suitably planned data collection form, base line survey, first visit and second visit was conducted using ten point questionnaires. Based on responses awareness was provided for appropriate use through FDCs educational tool. The data obtained were entered in Microsoft excel and graph pad instat software, the score of base line was compared with second follow up using Wilcoxon matched pair test. Out of...
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Posted by admin on Apr 30, 2016 in |
Introduction: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used drugs to manage a variety of acute and chronic conditions associated with pain and/or inflammation. In the Indian market, various NSAIDs group of drugs are available in different formulations. This creates a lot of problem with physician to decide the drug of choice for individual patients. Objective: To evaluate price variation of different single NSAIDs formulations available in Indian market. Materials and Methods: Cost of a particular drug in the same strength and dosage forms being manufactured by different companies was obtained from “Current Index of Medical Specialties” (CIMS) Jan-Apr, 2015 and “Indian Drug Review” (IDR) Issue 1,2015. Difference between the maximum and minimum cost of the same drug manufactured by different pharmaceutical companies was calculated and percentage cost variation was calculated. Spearman correlation analysis and regression coefficient was done to observe the correlation between no. of manufacturing companies and their percentage price variation with the help of IBM Statistical Package for Social Sciences (SPSS v.21). Results: For Oral preparations, Diclofenc(50mg...
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