Posted by admin on May 31, 2016 in |
Background – Drugs affecting gastrointestinal motility find place in the treatment of gastrointestinal motility disorders. Objective: To evaluate the effect of erythromycin base on the isolated tissue of rabbit jejunum, guinea pig ileum & rat colon in vitro and also to see whether it alters the contractile responses induced by acetylcholine. Materials and Methods: a) Rabbit jejunum experiment: After recording the baseline spontaneous rhythmic contractions, erythromycin was added to the organ bath and the responses were taken every 3 min for 30 min. The effect on frequency & the amplitude of spontaneous contractions were noted. (Erythromycin doses used – 1, 2, 10 & 20 µM; in six rabbits). b) Guinea pig ileum & rat colon experiment: Initially responses were recorded with acetylcholine (1-100µg). After giving wash, erythromycin was added to the organ bath and response was recorded for 30 seconds; then after a contact period of 3 min with erythromycin, responses to selected dose of acetylcholine were recorded again. (Erythromycin doses used – 100, 300 500 & 1000 µM;...
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Posted by admin on May 31, 2016 in |
In diabetes mellitus antioxidant vitamins are commonly employed to prevent or mitigate the long term complications of the disorder. Diabetic retinopathy, nephropathy, coronary and cerebral vascular insufficiency are examples of such complications. They are more common in Type 2 diabetes mellitus. The present study was conducted to observe the effect of Vit A on blood glucose level and its possible interactions with commonly prescribed oral antidiabetic agents i.e. glimepiride, gliclazide and metformin. The study was performed on healthy albino rabbits of either sex in which hyperglycemia was induced by giving oral glucose. We observed that Vit.A significantly lowered the blood glucose level but antagonized the effect of oral antidiabetic agents in co-administration therapy. It is therefore advisable that caution must be exercised when giving high doses of VitA to produce antioxidant effects in diabetic patients because it is not only unnecessary but may also have potentially deleterious effect on glycemic...
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Posted by admin on May 31, 2016 in |
A new complex has been synthesized of Zn (II) complex with 6-thioguanine and physicochemical characterized by amperometry, polarography elemental analysis, UV and IR spectroscopy. After Synthesis of metal complex, it was evaluated it for antibacterial activities against various pathogenic microorganisms such as; Streptococcus aureus, Proteus. M., klebsiella pneumonia and Asperginus Niger, Nigrosporan S.P. Sarcoma-180 tumor cell has been used for anticancer screening of metal complex for in vitro study. The result of pharmacological studies with M: L revealed that the complex is more potent as compared to the pure drug as regards to its anticancer...
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Posted by admin on May 31, 2016 in |
Effect of Vit. E on blood glucose and its possible interactions with oral antidiabetic agents (i.e. glimepiride, gliclazide and metformin) in rabbits have been reported in the present study. The study was performed on albino rabbits in which hyperglycemia was induced by giving glucose. We observed that Vit.E significantly lowered the blood glucose level but on co-administration with oral antidiabetic agents it antagonized the effect of the latter drugs. It is therefore advisable not to give inadvertent amount of Vit.E to diabetic patients just in a belief that antioxidants are helpful to diabetics and do not produce adverse...
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Posted by admin on May 31, 2016 in |
Kaempferol and quercetin are the flavonoids which are widespread in fruits and vegetables exhibiting important biological activities. A simple and precise HPTLC method was developed and validated for simultaneous determination of kaempferol and quercetin in herbal drug extracts of Podphyllum hexandrum and Cassia angustifolia. The HPTLC of flavonoids was performed with toluene: acetone: formic acid (7:3:0.25) as mobile phases on RP-18 F254 TLC plates. Densitometric determination of flavonoids was performed at λ = 254 nm in reflectance/ absorbance mode. The results obtained indicated that the developed method was precise and accurate. Good resolution of kaempferol and quercetin was obtained from other constituents present in methanolic and hydrolysed extracts of P. hexandrum and C. angustifolia using proposed HPTLC method. Kaempferol was quantified in free (8.01and 2.42 µg/mg) as well as bound (17.54 and 8.13 µg/mg) forms and quercetin in free (4.5 and 0.93 µg/mg) as well as bound form (6.17and 4.8 µg/mg) in P. Hexandrum and C. angustifolia. Kaempferol was found to be present in high concentration and quercetin was...
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