Posted by admin on Jul 31, 2016 in |
The present research has been focused on the development of pharmacophore mapping and docking studies of indole/benzoximidazole-5-carboximidine derivatives as anti-cancer agents that can explore basic pharmacophore responsible for biological activity of structurally diverse compounds and also their binding affinity to the urokinase-type plasminogen activator (uPA). For pharmacophore mapping, a highly predictive pharmacophore based 3D-QSAR model was generated. Molecular docking experiments were carried out by means of the Glide module of the Schrodinger. A cubing receptor grid was centred around the co-crystallized ligand where the active binding site is present. The XP (extra precision) scoring function of GLIDE 6.0 was used. The scoring function of GLIDE docking program is presented in the G-score form which indicates the binding affinity of the designed compound to the receptor. A five point pharmacophore (APRRR) with one acceptor atom, one positively charged group and three aromatic rings as pharmacophore was developed. The generated best pharmacophore hypothesis yielded a statistically significant QSAR model, with a correlation coefficient of R2 = 0.8548 for training set molecules....
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Posted by admin on Jul 31, 2016 in |
Numerous carbazole derivatives were designed by the Chemsketch software followed by 3D optimization. Docking studies were performed using AUTODOCK 4.2.6 software to check their binding interactions with eukaryotic topoisomerase-I, based on the crystal structure of Human Topoisomerse-I-DNA complex (PDB ID: 1A35). Results of docking studies of designed carbazole derivatives were compared on the basis of their minimum binding energy with a well known topoisomerase-I inhibitor i.e. Adriamycin,. Above results were used to find out active compounds and two series of such active compounds i.e. 2-[(4, 5-dihydro-2-substitutedphenyl)imidazol-1-ylamino]-1-(9H-carbazol-9-yl)ethanone (3a-3e) and 2-(9H-carbazol-9-yl)-N’-[{(4-substitutedphenyl)(piperazin-1-yl)} methyl] aceto hydrazide (6a-6e) were synthesized. All the synthesized compounds were characterized by IR, 1H NMR, 13C NMR, MASS spectrometry and elemental analysis and also screened for their in vitro anticancer activity against human breast cancer cell line (MCF 7) by sulphorodamine B (SRB) assay method. GI50 was measured by using 10, 20, 40 and 80 µg/ml concentrations of tested compounds along with the standard i.e. Adriamycin. Results revealed that the tested compounds 3a, 3d, 6c, 6d and 6e were...
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Posted by admin on Jul 31, 2016 in |
A reverse-phase liquid chromatographic (RP-HPLC) method was developed for the determinations of loperamide hydrochloride and norfloxacin in their marketed formulation and bulk. The separation was carried out using mobile phase of triethylamine and acetonitrile (50:50%) with pH 4.The pH adjusted with orthophosphoric acid. The column used was Capcell pack C18 Column (250mn x 4.6mm, 5mm) and flow rate of 1 ml/min. Detection carried out at 213 nm. The retention time loperamide hydrochloride 5.6 and norfloxacin were found to be 2.1 min respectively. Developed method was validated according to ICH guideline. Linearity was observed at concentration rang of 2-6 mg/ml for loperamide hydrochloride and 200-600 mg/ml for norfloxacin. The regression equation were found to be Y=48615x-435565 and Y=72087x-14016 the correlation coefficient (r2) 0.9996 and 0.9988 norfloxacin and loperamide hydrochloride respectively. The percentage RSD for the method precision was found to be less than 2%. The accuracy is found in 98-101 %. The proposed method is precise, accurate, selective and rapid for simultaneous determination of loperamide hydrochloride and...
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Posted by admin on Jul 31, 2016 in |
Azima tetracantha is a small flowering shrub belongs to salvadoraceae family and commonly known as mulluchangu or mulsangu in Tamil. It is an best known medicinal plant ,which have a central role in the treatment of disease from pre historical period. From the plant lot of phytochemicals has been reported such as alkaloids, steroids ,tannins, terpenoids, flavonoids, glycosides, phenols etc and also possesses activities such as antioxidant, antifungal, antibacterial , hepatoprotective, antidiabetic and antihyperlipidemic etc .In the present study preliminary evaluation and isolation of an active pharmacological compound euphol was obtained from the chloroform extract of the leaves of Azima tetracantha was carried out. The compound euphol is a tetracyclic triterpene alcohol which produces antinociceptive effects in inflammatory and neuropathic pain. The compound was isolated by column chromatography and characterized by chemical test, TLC, FTIR, NMR, C 13 NMR spectrum. In future it may be a promising molecule for the management of neuropathic...
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Posted by admin on Jul 31, 2016 in |
The Wheat, Maize and Cotton are the major crops in the agriculture field specially in Maharashtra, India. So for better productivity this study aims to prepare steam soluble aroma compounds called hydrosols. Hydrosols of different plant species (Ipomea carnea Leaves and Flowers, Lantana Camera Leaves) were prepared by steam distillation method. The purpose of this study was to determine the effect on the growth of Wheat, Maize and Cotton crops by using Ipomea carnea leaves and flowers and Lantena camera leaves. In this study the wheat, maize and cotton crops were germinated in different pots and continuous dosing of hydrosol was applied with fixed quantity and result was observed after...
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