Posted by admin on May 31, 2016 in |
High performance thin layer chromatography (HPTLC) offers many advantages over HPLC. It reduces the cost of analysis as compared to HPLC. The mobile phase consumption per sample is extremely low in HPTLC, hence reducing the acquisition and disposal cost. Considering the cost and suitability of analysis for estimation of cinnarizine in bulk and its marketed formulation, HPTLC method was developed and validated. The Camag HPTLC system, employed with software win CATS (ver.1.4.1.8) was used for proposed analytical work. Planar chromatographic development was carried out with the help of Silica Gel 60 F254precoated TLC plates. Sample application was facilitated by Linomat 5 applicator. After sample application plates were subjected for ascending development in twin trough chamber of 10×10 cm dimension, using 10 ml of solvent system. The optimized mobile phase was composed of toluene: ethanol (7.5:2.5 v/v). In post development, the plates were air dried and then scanned densitometrically using a UV detector at 254 nm in absorbance mode. In HPTLC densitogram well defined peak was obtained for cinnarizine with...
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Posted by admin on May 31, 2016 in |
The present study explores the nutritional properties of R. mucronata leaves, an edible red mangrove which has been proven as an antidiabetic and as an alternative source of tea leaves and animal feed. Proximate composition, amino acid profiles and physico-chemical properties were analyzed to evaluate the nutritional property. Nutritional evaluation of R.mucronata illustrates it as a rich source of essential aminoacids and unsaturated fatty acid (alpha linoleic and linoleic acid). Total dietary fiber content, protein, ash, carbohydrate and lipid content was found to be 11.9 ± 0.2, 2.445 ± 0.179, 13.5 ± 0.0021, 79.277± 0.079 and 0.749 ± 0.049 % DW respectively. Mineral and vitamin analysis per 100 g (DW) showed the presence of sodium (80.804 ± 4.0 g), calcium (22.32± 1.1g), potassium (7.96 ± 3.9 g), iron (53.6 ± 0.5mg), magnesium (38.15 ± 0.38 mg) and Vitamin A (2.2 ± 0.02 mg), Vitamin C (3.23 ± 2.3g), Vitamin E (1.180± 1.8 g), vitamin B1 and B2 (18.04 ±0.1 and 0.89 ± 0.08 mg). Swelling, water-holding and oil-holding capacity...
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Posted by admin on May 31, 2016 in |
A series of 2,4-diaminotriazines were synthesized as Mycobacterium tuberculosis(Mtb) dihydrofolatereductase (DHFR) inhibitors. These derivatives were evaluated in whole cells by employing resazurinmicrotitre plate assay (REMA) against MtbH37Rv. Further, these were tested against other gram positive and gram negative bacterial strains to check specificity for Mtb. Cytotoxicity assessment was performed using HepG2 cell line and the compounds were found to be non-cytotoxic. The results indicated that some of the derivatives exhibited promising activity. The most active compound in the REMA assay was selected for DHFR enzyme assay against both the Mtb and human enzymes. The enzyme assay results indicated that this derivative exhibited selectivity towards the pathogenic enzyme. The most active compound in the whole cell assay against MtbH37Rv showed low cytotoxicity, was specific towards Mtb and displayed selectivity in the DHFR enzyme assay. Thus this study provides promising insight for design of potent and selective Mtb DHFR...
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Posted by admin on May 31, 2016 in |
Bacteriological qualities of three different brands of chloroquine syrup (EZR, DMR and MBR) commonly sold in Calabar municipality, Nigeria were evaluated using standard microbiological techniques. Each brand was evaluated in duplicates and examined visually for possible abnormalities such as colour, turbidity and un-usual odour. This was followed by determination of viable cell count and identification of bacterial isolates. Chloroquine syrups sample EZR and MBR had mean bacterial count of 1.0x103cfu/ml respectively and DMR had 3.0x103cfu/ml. The isolates were staphylococcus aureus (47%), Escherichia coli (38%) and Pseudomonas aeruginosa (15%). All strain of S. aureus was sensitive to gentamycin (CN), ciprofloxacin (CPX), septin (SXT), Erythromycin (E) and resistance to Taravid (OFX), Amoxacillin (AM) and Ampicillin (AP). Escherichia coli strains isolated showed sensitivity to ciprofloxacin (CPX), Taravid (OFX) and septin (SXT) and was resistance to gentamycin (CN), Amoxacillin (AM) and Ampicillin (AP). P. aeroginosa strains were sensitive to Ciprofloxacin (CPX) and Taravid (OFX), and was resistant to Gentamycin (CN). The colony forming unit (cfu) for the sample DMR found in the study...
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Posted by admin on May 31, 2016 in |
This study compares the performance of Mycobacterium Growth Indicator Tube (MGIT) culture against conventional Lowenstein Jensen (LJ) media and direct AFB smear examination in pulmonary and extra pulmonary tuberculosis specimens for isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-320) media at a tertiary care centre in North India A total of 132 consecutive clinical samples were obtained from suspected cases of TB for smear-microscopy and culture on LJ and BACTEC-MGIT-320. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol. A total of 132 specimens were cultured on LJ and the BACTEC-MGIT-320. The mean (range) time to detection was 14.2 (3–40) and 23.2 (8–42) days for MGIT-320 and LJ, respectively 69 (52.3%) were positive with either MGIT or culture of which 45 (65.2%) were smear positive and 24(34.8%) were smear negative. On comparing MGIT and LJ culture, 65 (94.2%) were positive with MGIT-320 whereas 62(89.8%) LJ cultures were positive DST was conducted on both MGIT-320 and LJ media in the 58 concordant culture-positive isolates. Thirty...
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