Posted by admin on Jun 30, 2016 in |
Biofilm formation is prevailing in almost all bacteria. They are responsible to cause infections like Peritonitis, Cystic fibrosis, Endocarditis, Otitis media, UTIs. The infections caused by the biofilms forming bacteria that colonize the indwelling medical devices are a major source of patient morbidity and implant failure. The study on biofilm formation on indwelling medical devices is important from a public health perspective. In the present study we have isolated various organisms on indwelling medical devices, and tested them for biofilm formation and also antibiotic susceptibility. In the present study a total of 100 isolates are identified by standard microbiological procedures, evaluated for biofilm production from various indwelling medical devices by two different methods, Tube method and by using Congo red agar. The Results 48% of isolates showed biofilms production, Klebsiella being the predominant isolate 20 (42%). Gram negative isolates were sensitive to Amikacin and Nitrofurantoin 22 (46%). Gram positive isolates were sensitive to Vancomycin and tetracycline 11 (23%). Congo red agar method was considered to be superior to test...
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Posted by admin on Jun 30, 2016 in |
Introduction: Utilization of skilled health care is effective in reduction of maternal and newborn morbidity and mortality. Sufficiently trained health workers can competently handle and manage obstetric complications. Health workers, despite having undergone a formal training, require regular refreshers to keep up to date with new, evidence-based information. Methods: This is an evaluation report following Emergency Obstetrics and Neonatal Care skills – based training by Maternal and Infant Survival and Healthcare Advancement (MAISHA), a project by Dedan Kimathi University of Technology and College of The Rockies, Canada in collaboration with Liverpool School of Tropical Medicine and the Ministry of Health among health care workers in Migori and Nyeri Counties’ health facilities. Knowledge and skills pre-test was done before the training and a similar post-test was administered after the training to assess the level of knowledge and skills of each participant. Follow up was done to the trained participants four months after the training to assess the level of retention of the skills. Findings: The pre-test findings indicated that the...
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Posted by admin on Jun 30, 2016 in |
Colorants are mainly used to impart a distinctive appearance to the pharmaceutical dosage forms. The present study was carried out to develop aluminium lake color of a dye obtained from black carrot using different adsorbents (Aluminium oxide or aluminium hydroxide) in different ratio by simply mixing them to get dye adsorbed onto the surface of adsorbent. Adsorption experiment was carried out for 60 minutes. Aluminium oxide at a concentration level of 30% w/v found to be the choice of adsorbent after optimization because maximum adsorption of dye from reaction mixture after 60 minutes and maximum % yield. After optimization of adsorbent the effect of pH and temperature also studied by formulating lake by varying these two parameters. It was observed that a higher temperature (50oC) and a lower pH (3) favor the adsorption. The lake then characterized for various physicochemical properties like angle of repose, Carr’s index, hausner’s ratio, loss on drying, particle size and limit test for heavy metals. The lake was found to follow pseudo second order...
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Posted by admin on Jun 30, 2016 in |
This paper describes simple, sensitive, accurate, rapid, precise and economical Chemometrics assisted and RP-HPLC methods for simultaneous estimation of Linezolid(LNZ) and Cefuroxime axetil(CEF) in bulk and tablet. Chemometrics offers a useful approach for an estimation of combined dosage form. The measurement carried out at wavelength 240 to 290nm with interval of 4nm for Linezolid and Cefuroxime axetil respectively. Chemometrics method were found to be linear (R2=0.999) for all four methods CLS, ILS, PLS and PCR for LNZ and CEF respectively. HPLC method by using mobile phase Acetonitrile: water 65:35 with Thermo Synchronies C-18 (250mm x 4.6mm, 5μm) flow rate 0.8ml/min and detection wavelength 267nm. Retention time was found to be 3.573 min for LNZ and 4.273 min for CEF. HPLC method was found to be linear (R2=0.999) for 6-30µg/ml for LNZ and 5-25µg/ml for CEF respectively. The applied methods Chemometrics and RP-HPLC were found to be successful for simultaneous estimation of LNZ and CEF in marketed dosage...
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Posted by admin on Jun 30, 2016 in |
Objective: Present study was aimed to evaluate the total phenolic, flavonoid content and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of whole plant of Catharanthus pusillus. Methods: Antioxidant activity have been tested using various antioxidant model systems viz., DPPH, hydroxyl, superoxide, ABTS and reducing power. Results: Methanol extract of Catharanthus pusillus is found to possess higher DPPH, hydroxyl, superoxide radical scavenging activity, while ethyl acetate extract is found to possess higher ABTS radical cation scavenging activity. Methanol extract of whole plant of Catharanthus pusillus shows the highest reducing ability. Conclusion: The study indicates the significant free radical scavenging potential of Catharanthus pusillus whole plant which can be experimented for the treatment of various free radicals mediated...
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